FastQCFastQC Report
Thu 26 May 2016
SRR1511831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879506
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45250.24075475151449371No Hit
GTCCTACAGTGGACATTTCTAAATT40360.21473727670994397No Hit
GTCCTAAAGTGTGTATTTCTCATTT38260.2035641280208736No Hit
CTGTAGGACGTGGAATATGGCAAGA36210.19265700668154292No Hit
CTTTAGGACGTGAAATATGGCGAGG32960.1753652289484577No Hit
GGTATCAACGCAGAGTACTTTTTTT30500.16227668334126094No Hit
TATCAACGCAGAGTACTTTTTTTTT28920.15387021908948414No Hit
GTCCTACAGTGTGCATTTCTCATTT19560.10406989921819883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.013.5065351
TAGGACC7300.013.3271424
CGGTAGG904.253354E-612.2113881
GCCCTAA957.7724235E-611.5686841
GTGGTAT3550.011.2576181
GGCGAGG10450.010.84702619
GGACCTG14550.010.7762526
CGTTAGG650.007324657410.7596851
TAATACT2200.010.7334664
AGGACCT14800.010.7218615
TGTAGGA22000.010.6269362
CCAACGA803.9755733E-410.62669719
AGATCGT803.9775277E-410.626136
GTCCTAG2950.010.4991591
CGTTTCC901.0091266E-410.49466514
GTCCTAA11550.010.4669041
GGACGTG22050.010.4092716
TGGTATC3650.010.4017312
ACCTTTT6650.010.36841315
TGGCGAG23450.010.31199818
Produced by FastQC (version 0.11.5)