##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511831_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1879506 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.23705643929841 31.0 30.0 34.0 23.0 34.0 2 26.520448458265097 31.0 26.0 34.0 10.0 34.0 3 28.25259669296081 31.0 28.0 34.0 16.0 34.0 4 32.604633877199646 35.0 32.0 37.0 26.0 37.0 5 32.96727970009141 35.0 35.0 37.0 26.0 37.0 6 33.16437510707601 37.0 35.0 37.0 27.0 37.0 7 33.13808575232002 37.0 35.0 37.0 26.0 37.0 8 33.14866991645677 37.0 35.0 37.0 26.0 37.0 9 34.67975893665676 39.0 35.0 39.0 27.0 39.0 10 34.539519958967944 39.0 35.0 39.0 27.0 39.0 11 34.539895589585775 39.0 35.0 39.0 27.0 39.0 12 34.42212155747308 39.0 35.0 39.0 27.0 39.0 13 34.413031934987174 39.0 35.0 39.0 27.0 39.0 14 35.48262947817139 40.0 36.0 41.0 26.0 41.0 15 35.43024975711703 40.0 36.0 41.0 26.0 41.0 16 35.38548480292162 40.0 36.0 41.0 26.0 41.0 17 35.332830009587624 40.0 36.0 41.0 25.0 41.0 18 35.298515141744694 40.0 36.0 41.0 25.0 41.0 19 35.31964941851742 40.0 36.0 41.0 25.0 41.0 20 35.249044695787084 40.0 36.0 41.0 25.0 41.0 21 35.13967819203557 40.0 36.0 41.0 24.0 41.0 22 35.04045371496553 40.0 35.0 41.0 24.0 41.0 23 34.92095582562652 40.0 35.0 41.0 23.0 41.0 24 34.803922945710205 39.0 35.0 41.0 22.0 41.0 25 34.699933386751624 39.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.008793570874829 1101 2 3.5193876255422225 1101 3 2.1566404058311726 1101 4 1.2798845111036101 1101 5 0.7936116544130698 1101 6 0.6975283804400334 1101 7 0.5532319341859093 1101 8 0.453435647177173 1101 9 0.6342520490896391 1101 10 0.7699721952832448 1101 11 0.7811724785204177 1101 12 0.6958361144374834 1101 13 0.606802688137428 1101 14 0.7376916388399124 1101 15 0.9314692851513726 1101 16 0.857679253657146 1101 17 0.849807555084638 1101 18 1.0607281941263125 1101 19 1.049891300819226 1101 20 1.0093032606626267 1101 21 1.0764667924959355 1101 22 1.0414640648918692 1101 23 0.8431311836492625 1101 24 0.984506762633508 1101 25 1.1402600488704877 1102 1 1.3342831051274011 1102 2 4.036472481186824 1102 3 2.433673689142992 1102 4 1.4237655420029114 1102 5 1.0298523171905245 1102 6 1.0421233962294991 1102 7 0.9368300470520836 1102 8 0.7409308768884344 1102 9 0.904607688369147 1102 10 0.9683538700765055 1102 11 0.9776878491129324 1102 12 1.0151048880893683 1102 13 1.176280898370706 1102 14 1.2193775311005695 1102 15 1.1411772598578196 1102 16 1.1836287121628573 1102 17 1.2031608970235013 1102 18 1.1610137272659458 1102 19 1.2724839964618795 1102 20 1.23362770415428 1102 21 1.3367170216978579 1102 22 1.3226784777776501 1102 23 1.4622652489083308 1102 24 1.080161124615941 1102 25 1.2750801918803276 1103 1 1.2371704593310753 1103 2 3.7742975004934394 1103 3 2.3887752869776975 1103 4 1.4141602238608968 1103 5 1.011957336319945 1103 6 1.070198833046291 1103 7 1.0648665014862573 1103 8 0.7246262000361412 1103 9 0.8674991180037281 1103 10 1.018718665441149 1103 11 1.0772132681366315 1103 12 0.7587329666270577 1103 13 0.8419944162501807 1103 14 1.1958714497012153 1103 15 1.0004434755691989 1103 16 1.0365285436821807 1103 17 1.1411131997157895 1103 18 1.2248252297267115 1103 19 1.1769107117974613 1103 20 1.3055273127061895 1103 21 1.3507772583386384 1103 22 1.5099681537956613 1103 23 1.5269678827293873 1103 24 1.729527144086454 1103 25 1.5432044157396092 1104 1 1.3111535572431379 1104 2 3.9346415446531573 1104 3 2.2457814071827897 1104 4 1.5161105834439823 1104 5 1.2838254293515519 1104 6 0.9794559938545362 1104 7 0.9313474396559158 1104 8 0.9984065065512695 1104 9 1.2177974148925799 1104 10 1.2341550736492906 1104 11 1.1171972523845355 1104 12 1.2040367890906225 1104 13 1.3544171423499662 1104 14 1.4797183996961678 1104 15 1.5791478545170392 1104 16 1.5391496855968896 1104 17 1.4217948649052303 1104 18 1.2621039262520526 1104 19 1.45482397990844 1104 20 1.3853725685655434 1104 21 1.4863445693752055 1104 22 1.3735271287875293 1104 23 1.3643729382095202 1104 24 1.3912091065910843 1104 25 1.5138326202924617 1105 1 1.2533738950200721 1105 2 3.891630728200763 1105 3 2.2243010370408385 1105 4 1.5455596533450446 1105 5 1.1178314964747003 1105 6 0.9784281146066292 1105 7 0.8073374323289855 1105 8 0.856595739120813 1105 9 0.9687650050692156 1105 10 0.8289212574762672 1105 11 0.7557049693856612 1105 12 0.5490727912201763 1105 13 0.8386550094214584 1105 14 0.8285895115452462 1105 15 1.0246518052631757 1105 16 0.9353753920662058 1105 17 1.0949177333735847 1105 18 1.1057058391616863 1105 19 1.187090756742677 1105 20 1.1306011662746869 1105 21 1.1786146538721454 1105 22 1.1227858697145123 1105 23 1.0319607428175814 1105 24 1.3135231790486657 1105 25 1.3321467270126846 1106 1 1.0619456104298344 1106 2 3.6942949184964853 1106 3 2.0608431252378487 1106 4 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1107 17 0.4196269040481013 1107 18 0.4625778020199931 1107 19 0.48438542582100297 1107 20 0.4937773253549267 1107 21 0.5488173939331062 1107 22 0.5375791982134288 1107 23 0.4017522792466153 1107 24 0.4694277020107762 1107 25 0.6168246684383902 1108 1 1.0591531351272252 1108 2 3.6930202986459797 1108 3 2.1422738337815233 1108 4 1.3534716061000083 1108 5 0.9450916042902122 1108 6 0.8474003634207179 1108 7 0.7600396579120599 1108 8 0.6461517040570897 1108 9 0.7124694357663373 1108 10 0.7398854205742609 1108 11 0.7906051225011979 1108 12 0.7839259281222724 1108 13 0.8200344436716094 1108 14 0.9212525688683755 1108 15 0.9119183666349713 1108 16 0.8707075401619164 1108 17 0.8212118635870453 1108 18 0.8328938137371793 1108 19 0.8720204514143859 1108 20 0.7976468229508527 1108 21 0.8686544567615897 1108 22 0.6785496910064239 1108 23 0.6637728424311788 1108 24 0.902814208516034 1108 25 0.9064926787468721 1109 1 0.8579087546492019 1109 2 2.932190657756344 1109 3 1.7376348307952263 1109 4 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-0.5073680770996987 2314 8 -0.5189513343367764 2314 9 -0.6359255076604882 2314 10 -0.548702618317833 2314 11 -0.6800715579385042 2314 12 -0.5575961489985204 2314 13 -0.6686072404331611 2314 14 -1.5614164783899156 2314 15 -1.1164658204489513 2314 16 -0.9199020905870299 2314 17 -0.9966080386503577 2314 18 -0.8502725096274304 2314 19 -0.7996592443503729 2314 20 -0.8773737546975156 2314 21 -0.8129885063651727 2314 22 -0.6874730966063112 2314 23 -0.72549279146277 2314 24 -0.7855228035411699 2314 25 -0.7132521720634699 2315 1 -4.591046951631622 2315 2 -13.575492099024142 2315 3 -7.16925281671422 2315 4 -3.3855114794893595 2315 5 -2.223212986554035 2315 6 -1.566360472244785 2315 7 -1.4687477033690293 2315 8 -1.4212040426471795 2315 9 -1.4801249590416816 2315 10 -1.5134441786083244 2315 11 -1.5310324763864003 2315 12 -1.4247531650672869 2315 13 -1.4572213476597682 2315 14 -1.6303171446736897 2315 15 -1.7171583690488674 2315 16 -1.6433569069130698 2315 17 -1.6269396983730076 2315 18 -1.596990042143318 2315 19 -1.6740859500473704 2315 20 -1.5228607274529367 2315 21 -1.7728238480676524 2315 22 -1.5912995148069982 2315 23 -1.6351412460338821 2315 24 -1.749672042969216 2315 25 -1.8291717806267442 2316 1 -1.868347851240447 2316 2 -1.5832611765993825 2316 3 -1.3255828959291023 2316 4 -1.491075309153306 2316 5 -1.6708025286585162 2316 6 -1.893426417220347 2316 7 -1.831617321018598 2316 8 -1.965885808264261 2316 9 -2.022438039207195 2316 10 -2.161034001654002 2316 11 -2.1708128365600317 2316 12 -2.0128064568025934 2316 13 -2.025497959551096 2316 14 -2.4281353553521967 2316 15 -2.960476446104721 2316 16 -2.6280017147159285 2316 17 -2.638057419406131 2316 18 -2.532819210230791 2316 19 -2.513097514640492 2316 20 -2.6269784123148767 2316 21 -2.688998018966288 2316 22 -2.6267481480456425 2316 23 -2.461708520388065 2316 24 -2.5370039479506943 2316 25 -2.7426208219324764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80005.0 3 2.0 4 721.0 5 3472.0 6 4250.0 7 3802.0 8 3514.0 9 3410.0 10 3243.0 11 3175.0 12 2966.0 13 2930.0 14 3061.0 15 3204.0 16 3304.0 17 3598.0 18 4030.0 19 5137.0 20 6390.0 21 8163.0 22 10155.0 23 12271.0 24 13936.0 25 15773.0 26 17724.0 27 20202.0 28 23210.0 29 27507.0 30 33861.0 31 42667.0 32 54428.0 33 70788.0 34 95302.0 35 135882.0 36 208950.0 37 280183.0 38 668290.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57714527115103 19.511669555723685 12.83119074905853 25.079994424066747 2 15.784689865000622 21.502873386938372 40.54877722766984 22.16365952039117 3 18.316355467872942 25.458498137276496 30.215439588913256 26.009706805937306 4 11.955801152004836 17.368180787930445 39.536239841745655 31.13977821831907 5 12.548098000740623 38.3924200956001 36.433597091986364 12.625884811672917 6 30.418746797666813 38.19059329424115 18.802238267954746 12.588421640137293 7 26.979270084798877 31.99186381953556 23.327831887740714 17.70103420792485 8 24.023280585430427 37.92916862196768 21.296021401368233 16.751529391233653 9 26.330216556903785 15.118493902121088 20.950983928755747 37.60030561221938 10 14.631770263037202 29.150106464145363 35.56615408516919 20.651969187648245 11 33.339451962377346 22.44180119669743 25.966982813569096 18.251764027356124 12 23.059410291853286 24.879569418772803 32.825061478920524 19.235958810453386 13 28.766434530830757 22.569211115917344 26.201820374854417 22.46253397839748 14 20.297727168734763 22.86441224449403 28.48514450073583 28.35271608603537 15 22.21817860650618 32.22979868114281 23.80551059693345 21.746512115417563 16 21.217649744134896 28.38862977825024 29.087483626016624 21.30623685159824 17 21.023743624613857 29.21222414117842 28.577319214639687 21.18671301956803 18 20.73970500759242 27.695575326708187 31.156750763232466 20.407968902466926 19 23.031509992822556 27.123238692864426 28.280089534498288 21.565161779814726 20 23.09849188212791 27.726117919973188 28.99071293086707 20.184677267031837 21 22.355934475911575 27.143983587054375 27.844970875108803 22.655111061925243 22 22.737012145710214 28.79379854200398 28.009677407340206 20.4595119049456 23 21.51560440776568 28.415520928161254 28.70644921178547 21.3624254522876 24 22.618511118855633 27.172413426161167 29.34680879931257 20.86226665567063 25 22.169235091184213 27.874180895688834 28.870620359118316 21.085963654008637 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 279.0 1 279.0 2 399.0 3 519.0 4 519.0 5 519.0 6 1350.5 7 2182.0 8 2182.0 9 2182.0 10 3257.5 11 4333.0 12 4333.0 13 4333.0 14 7042.0 15 9751.0 16 9751.0 17 9751.0 18 17706.0 19 25661.0 20 25661.0 21 25661.0 22 40324.5 23 54988.0 24 54988.0 25 54988.0 26 79242.5 27 103497.0 28 103497.0 29 103497.0 30 132697.5 31 161898.0 32 161898.0 33 161898.0 34 181508.0 35 201118.0 36 201118.0 37 201118.0 38 212138.0 39 223158.0 40 223158.0 41 223158.0 42 230973.5 43 238789.0 44 238789.0 45 238789.0 46 239731.0 47 240673.0 48 240673.0 49 240673.0 50 227696.0 51 214719.0 52 214719.0 53 214719.0 54 187286.5 55 159854.0 56 159854.0 57 159854.0 58 134941.5 59 110029.0 60 110029.0 61 110029.0 62 88487.0 63 66945.0 64 66945.0 65 66945.0 66 51158.5 67 35372.0 68 35372.0 69 35372.0 70 25770.5 71 16169.0 72 16169.0 73 16169.0 74 11219.5 75 6270.0 76 6270.0 77 6270.0 78 4263.5 79 2257.0 80 2257.0 81 2257.0 82 1496.0 83 735.0 84 735.0 85 735.0 86 483.5 87 232.0 88 232.0 89 232.0 90 143.0 91 54.0 92 54.0 93 54.0 94 34.0 95 14.0 96 14.0 97 14.0 98 12.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.4632972706658025 3 0.0 4 0.0 5 5.320546994795441E-4 6 9.044929891152249E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5961640984386323E-4 14 0.0 15 0.0 16 0.0 17 6.384656393754529E-4 18 0.0 19 3.7243828963568084E-4 20 5.852601694274985E-4 21 7.448765792713617E-4 22 0.004096821185992489 23 2.6602734973977203E-4 24 0.0021814242678661307 25 7.448765792713617E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1879506.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.89521418875476 #Duplication Level Percentage of deduplicated Percentage of total 1 86.82157242669753 65.89341835528695 2 9.57815036438648 14.538715468744225 3 1.9593612285751179 4.461184203475508 4 0.6572313901019855 1.995228684934529 5 0.30311325566724406 1.1502422731158137 6 0.15829431899809562 0.7208268747134124 7 0.10704788547863837 0.5687095537798186 8 0.07294392812095325 0.44288760388071036 9 0.05488278886090449 0.3748806914287095 >10 0.25143509504684747 3.3767284051348767 >50 0.017720113504438067 0.9443708059834024 >100 0.014769077908314738 2.202597021326425 >500 0.0022714296511979915 1.1966065291528443 >1k 0.0012066970021989301 2.1336035290427833 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4525 0.24075475151449371 No Hit GTCCTACAGTGGACATTTCTAAATT 4036 0.21473727670994397 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3826 0.2035641280208736 No Hit CTGTAGGACGTGGAATATGGCAAGA 3621 0.19265700668154292 No Hit CTTTAGGACGTGAAATATGGCGAGG 3296 0.1753652289484577 No Hit GGTATCAACGCAGAGTACTTTTTTT 3050 0.16227668334126094 No Hit TATCAACGCAGAGTACTTTTTTTTT 2892 0.15387021908948414 No Hit GTCCTACAGTGTGCATTTCTCATTT 1956 0.10406989921819883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1080 0.0 13.506535 1 TAGGACC 730 0.0 13.327142 4 CGGTAGG 90 4.253354E-6 12.211388 1 GCCCTAA 95 7.7724235E-6 11.568684 1 GTGGTAT 355 0.0 11.257618 1 GGCGAGG 1045 0.0 10.847026 19 GGACCTG 1455 0.0 10.776252 6 CGTTAGG 65 0.0073246574 10.759685 1 TAATACT 220 0.0 10.733466 4 AGGACCT 1480 0.0 10.721861 5 TGTAGGA 2200 0.0 10.626936 2 CCAACGA 80 3.9755733E-4 10.626697 19 AGATCGT 80 3.9775277E-4 10.62613 6 GTCCTAG 295 0.0 10.499159 1 CGTTTCC 90 1.0091266E-4 10.494665 14 GTCCTAA 1155 0.0 10.466904 1 GGACGTG 2205 0.0 10.409271 6 TGGTATC 365 0.0 10.401731 2 ACCTTTT 665 0.0 10.368413 15 TGGCGAG 2345 0.0 10.311998 18 >>END_MODULE