FastQCFastQC Report
Thu 26 May 2016
SRR1511831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879506
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58180.30954942415719877No Hit
GTCCTACAGTGGACATTTCTAAATT43630.2321354653829251No Hit
CTGTAGGACGTGGAATATGGCAAGA41500.22080270028401078No Hit
GTCCTAAAGTGTGTATTTCTCATTT40890.21755716661718558No Hit
CTTTAGGACGTGAAATATGGCGAGG39770.2115981539830147No Hit
GGTATCAACGCAGAGTACTTTTTTT38380.20420259366024904No Hit
TATCAACGCAGAGTACTTTTTTTTT37760.20090385452347587No Hit
GTCCTACAGTGTGCATTTCTCATTT23400.12450079967821331No Hit
CTGAAGGACCTGGAATATGGCGAGA19830.10550644690679359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG500.001498801413.3011174
TATTCCG500.001498801413.3011175
GGTATCA11600.012.8267761
GCGCCAC751.4837722E-512.66267613
CCCCGTA853.958472E-612.29024415
CTAGGAC2500.012.1610213
TAGGACC6600.012.0919264
GGCGAGG10200.012.0115619
GTGGTAT3900.010.760661
CCCGTAC803.7824624E-410.68413416
GATATAC4350.010.5245331
TAGAAAT7300.010.4118344
GTATCAA23800.010.3794341
AATCCCG1659.331416E-1010.36092319
GACGTGA12650.010.3603727
TTAGGAC15900.010.2775943
CCGTGTC750.002654943410.130149
AGGACCT13150.010.1149185
GTCCTAA13250.010.0777371
AGGACGT24500.010.0437015
Produced by FastQC (version 0.11.5)