FastQCFastQC Report
Thu 26 May 2016
SRR1511830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013308
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT47880.23781756194283238No Hit
GTATCAACGCAGAGTACTTTTTTTT47320.23503606999028462No Hit
GTCCTAAAGTGTGTATTTCTCATTT45670.2268406026300993No Hit
CTGTAGGACGTGGAATATGGCAAGA45360.22530084815636753No Hit
CTTTAGGACGTGAAATATGGCGAGG39840.19788328462411117No Hit
GGTATCAACGCAGAGTACTTTTTTT31110.15452181186385788No Hit
TATCAACGCAGAGTACTTTTTTTTT28970.14389253904519328No Hit
GTCCTACAGTGTGCATTTCTCATTT27370.13594541918077113No Hit
CTGTAGGACCTGGAATATGGCGAGA21790.10822983865359896No Hit
CTGAAGGACCTGGAATATGGCGAGA21290.10574636369596703No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA400.00548668114.1676727
TGGACCG552.0584816E-413.7390325
GGACCGT552.0589153E-413.738696
GGTATCA9700.013.4936941
GCGACTA655.7559842E-513.07850116
TAAGCGA604.2979285E-412.59348713
TAGGACC8350.011.8776814
TAATACT2400.011.806984
GGCGAGG13550.011.7111319
GTCTTAC1403.792593E-911.4188731
AAATGTC7650.010.9884347
AATGTCC7800.010.8982098
CCACCTT7750.010.84664713
GTGGTAT3700.010.8016371
ATAGTAC1951.8189894E-1210.6565573
TCCAACG1606.020855E-1010.62628218
AGAGCGC803.9790955E-410.6257548
GTACTAA1706.493792E-1010.579251
CGGTAGG854.1433342E-410.579251
TGGCGAG28600.010.50243318
Produced by FastQC (version 0.11.5)