##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511830_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2013308 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.03219924621568 31.0 30.0 34.0 23.0 34.0 2 26.333351876613015 31.0 25.0 34.0 7.0 34.0 3 28.053937599214823 31.0 27.0 34.0 16.0 34.0 4 32.37675805192251 35.0 32.0 37.0 24.0 37.0 5 32.73744553739418 35.0 33.0 37.0 26.0 37.0 6 32.92496776449505 36.0 35.0 37.0 26.0 37.0 7 32.89525000645703 37.0 35.0 37.0 26.0 37.0 8 32.9105159270216 37.0 35.0 37.0 26.0 37.0 9 34.418066684282785 39.0 35.0 39.0 27.0 39.0 10 34.27764604322836 39.0 35.0 39.0 26.0 39.0 11 34.26842788088062 39.0 35.0 39.0 25.0 39.0 12 34.144322676907855 39.0 35.0 39.0 25.0 39.0 13 34.13132863923453 39.0 35.0 39.0 25.0 39.0 14 35.179817991087305 40.0 36.0 41.0 25.0 41.0 15 35.12334774411069 40.0 35.0 41.0 25.0 41.0 16 35.06975336113501 40.0 35.0 41.0 24.0 41.0 17 35.02015488936616 40.0 35.0 41.0 24.0 41.0 18 34.98384847226554 40.0 35.0 41.0 24.0 41.0 19 34.99960463078674 40.0 35.0 41.0 23.0 41.0 20 34.93200146227006 40.0 35.0 41.0 22.0 41.0 21 34.80942061522629 40.0 35.0 41.0 19.0 41.0 22 34.70792397387782 39.0 35.0 41.0 19.0 41.0 23 34.58595505506361 39.0 35.0 41.0 18.0 41.0 24 34.46555569242262 39.0 34.0 41.0 18.0 41.0 25 34.35720217671613 39.0 34.0 41.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9126229170853293 1101 2 3.492126017523425 1101 3 2.0462285031171987 1101 4 1.244624461210151 1101 5 0.6739186250432567 1101 6 0.658761361283851 1101 7 0.5428397366710485 1101 8 0.47992906161567817 1101 9 0.6664966015439049 1101 10 0.6317941223097066 1101 11 0.6259668713454261 1101 12 0.5699888967851621 1101 13 0.6151083513884075 1101 14 0.8251849117684813 1101 15 0.7925040914248882 1101 16 0.8666502694634133 1101 17 0.8151013352051066 1101 18 0.9104516893264858 1101 19 0.9818308452618751 1101 20 1.004357876565848 1101 21 0.8462423364566334 1101 22 0.8922034514908361 1101 23 0.7760404563976167 1101 24 0.8538950268802239 1101 25 0.956081656488081 1102 1 1.3521940999619488 1102 2 4.009402406109391 1102 3 2.301260810914524 1102 4 1.4925465562138882 1102 5 1.274002537075475 1102 6 1.1292751060414261 1102 7 1.058483170471284 1102 8 0.9026502422446541 1102 9 1.0199890236547233 1102 10 1.0669589245420354 1102 11 0.8978638326976096 1102 12 1.0534843657019124 1102 13 0.9690885954325736 1102 14 1.4016342673606985 1102 15 1.157528757233564 1102 16 1.2343404742320772 1102 17 1.1762071093185753 1102 18 1.111995071347664 1102 19 1.3327399755236513 1102 20 1.435578480299597 1102 21 1.2517312489134085 1102 22 1.2940028984039884 1102 23 1.2757202920365884 1102 24 1.0184055649994264 1102 25 1.2878475219391348 1103 1 1.2676688285353244 1103 2 3.6868972244760876 1103 3 2.2380120730239526 1103 4 1.5155247446239244 1103 5 1.1272986406171768 1103 6 0.9679544555271633 1103 7 1.0570901395244263 1103 8 0.8788303226817931 1103 9 0.8244072188220031 1103 10 0.98647077894951 1103 11 1.127568073515235 1103 12 0.9676193415642729 1103 13 1.1063901827341596 1103 14 1.3260087353905092 1103 15 1.0454242019443996 1103 16 1.0749985903916297 1103 17 1.2068381965194561 1103 18 1.3307749997469145 1103 19 1.279012123351265 1103 20 1.327643921566839 1103 21 1.373449926833473 1103 22 1.2622672624112923 1103 23 1.340205149972121 1103 24 1.4254428402724812 1103 25 1.3132021145880373 1104 1 1.6901576937882652 1104 2 4.194178204186105 1104 3 2.6010103668040045 1104 4 1.9397364488495867 1104 5 1.5855571883359403 1104 6 1.5814365476039498 1104 7 1.4090072364391943 1104 8 1.427143676253408 1104 9 1.6209201779687419 1104 10 1.5622110642058473 1104 11 1.486075537304231 1104 12 1.6382265638234301 1104 13 1.6795120482013033 1104 14 1.9817493160961916 1104 15 1.8309390593531631 1104 16 1.8248693313047113 1104 17 1.888768514792261 1104 18 1.7755185866496461 1104 19 1.9549965236260363 1104 20 1.8101865585999377 1104 21 1.8891973767363481 1104 22 1.9801500724299785 1104 23 1.9520696569041007 1104 24 2.0254155112083865 1104 25 1.8856352137777677 1105 1 1.5131110054060741 1105 2 4.080563437628015 1105 3 2.466869215881097 1105 4 1.7627601187735848 1105 5 1.1554863957192225 1105 6 1.0345648863661694 1105 7 0.9565477358963079 1105 8 1.0106815698473284 1105 9 1.0976081258514796 1105 10 1.0367682846184465 1105 11 1.1470084363270274 1105 12 1.0255386159406967 1105 13 1.0650885954325702 1105 14 1.4071234746195387 1105 15 1.4871058345974646 1105 16 1.253925574518611 1105 17 1.3821669947054005 1105 18 1.3179350904498648 1105 19 1.2809821913784774 1105 20 1.3527559397074285 1105 21 1.5479986796679412 1105 22 1.5246382374679825 1105 23 1.4421951726478568 1105 24 1.4529820692974198 1105 25 1.4880247903249995 1106 1 1.099218231513298 1106 2 3.6962529481175252 1106 3 2.209540544552425 1106 4 1.2566407714542365 1106 5 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1107 18 1.2542575710700135 1107 19 1.2961718554611608 1107 20 1.2578184720071732 1107 21 1.1551511344483032 1107 22 1.2243302271320644 1107 23 1.2289569297537994 1107 24 1.2887608806669277 1107 25 1.2766098032448951 1108 1 0.9667831528898816 1108 2 3.620118195973806 1108 3 1.964949933369887 1108 4 1.244592904025204 1108 5 0.8461881396155846 1108 6 0.6925866103322846 1108 7 0.5580815674857433 1108 8 0.5869183411477721 1108 9 0.543167069548538 1108 10 0.5721632024061876 1108 11 0.6052812813693791 1108 12 0.36171958255753367 1108 13 0.5478628854176222 1108 14 0.6936988607680163 1108 15 0.4674928799387672 1108 16 0.6028475476127824 1108 17 0.7126901636142193 1108 18 0.7113206410228585 1108 19 0.7333301734412601 1108 20 0.6176543951135116 1108 21 0.7124471101612144 1108 22 0.6670342514191532 1108 23 0.7841208328371962 1108 24 0.7819857563925865 1108 25 0.7777140778187714 1109 1 0.36804099092031706 1109 2 2.409206218893104 1109 3 1.2408000061595459 1109 4 0.7161844870336864 1109 5 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-0.8340992800679032 2314 10 -0.8850389204170455 2314 11 -0.8763528064746282 2314 12 -1.1566535361953711 2314 13 -1.0714095966124262 2314 14 -1.6280364557407196 2314 15 -1.4638328556395805 2314 16 -1.3933653857063817 2314 17 -1.3527787666445406 2314 18 -1.2987960197684316 2314 19 -1.291465324357425 2314 20 -1.1807130304278317 2314 21 -0.9578955214837919 2314 22 -0.9774304916066114 2314 23 -1.0820864933491947 2314 24 -1.1110433224261627 2314 25 -0.951589726884059 2315 1 -4.426533684684728 2315 2 -13.45527485688442 2315 3 -7.004280145877029 2315 4 -3.3838343278641325 2315 5 -2.1176510170982574 2315 6 -1.5124824619691388 2315 7 -1.2837853605637548 2315 8 -1.231504191437054 2315 9 -1.205434695572393 2315 10 -1.4679819193557648 2315 11 -1.2561610333400353 2315 12 -1.267356975584505 2315 13 -1.35329592884694 2315 14 -1.4293526932972966 2315 15 -1.45657391273771 2315 16 -1.452619391924344 2315 17 -1.410237269013514 2315 18 -1.3601286547797713 2315 19 -1.2562731316903495 2315 20 -1.2982193991529485 2315 21 -1.4491672632192802 2315 22 -1.4506425099987865 2315 23 -1.4147026296945597 2315 24 -1.5886375122264624 2315 25 -1.7808520618923964 2316 1 -1.5529384257433954 2316 2 -1.4296739888137253 2316 3 -1.0271012300454103 2316 4 -1.2537945562623776 2316 5 -1.4727852925245344 2316 6 -1.5689343159353903 2316 7 -1.4363584495358879 2316 8 -1.6062334033447776 2316 9 -1.4827275875001646 2316 10 -1.8266174091176595 2316 11 -1.9127240600767905 2316 12 -1.7669526616845204 2316 13 -1.8770471440731171 2316 14 -1.987999737097475 2316 15 -2.612204640098497 2316 16 -2.2085284074350966 2316 17 -2.179810251273416 2316 18 -2.144308822195619 2316 19 -2.0518659320686297 2316 20 -2.3096639373685477 2316 21 -2.2940298282436586 2316 22 -2.209275088019133 2316 23 -2.3838358460522855 2316 24 -2.166330288489945 2316 25 -2.3649718881109933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94047.0 3 2.0 4 831.0 5 4239.0 6 4949.0 7 4445.0 8 4095.0 9 3729.0 10 3766.0 11 3612.0 12 3452.0 13 3340.0 14 3520.0 15 3590.0 16 3724.0 17 4011.0 18 4648.0 19 5667.0 20 7236.0 21 9354.0 22 11459.0 23 13686.0 24 15942.0 25 17843.0 26 19978.0 27 22252.0 28 25721.0 29 30533.0 30 37655.0 31 47408.0 32 59633.0 33 77412.0 34 104348.0 35 146239.0 36 222744.0 37 297865.0 38 690333.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.608979848090804 19.36514432963064 12.733223133271213 25.292652689007344 2 15.83407502992181 21.355975482505592 40.523219218891036 22.28673026868156 3 18.458228944602613 25.266973557945434 30.072845287457262 26.201952209994694 4 12.044406519022424 17.214554355319702 39.18844011944521 31.552599006212663 5 12.648463017857283 38.27420309780579 36.32178278802521 12.75555109631172 6 30.71750538296673 37.90423162030403 18.61148018546711 12.766782811262134 7 27.170557112970293 31.803727993928398 23.140324282226068 17.885390610875238 8 24.116627957570326 38.0125147270065 21.14763364572137 16.723223669701802 9 26.26488346542109 15.113137185169881 20.864815517546248 37.75716383186278 10 14.834044269431205 29.024371829844213 35.314318524537725 20.82726537618685 11 33.553485110077546 22.099946952974907 25.99641982250108 18.350148114446473 12 23.39358905840537 24.534696131938084 32.73219994158867 19.339514868067877 13 29.071516273747033 22.39627001188097 26.03054481588474 22.501668898487264 14 20.201727703858523 22.977259316507954 28.16126494306882 28.659748036564697 15 22.130146008459707 32.570426382848524 23.477878198467398 21.821549410224367 16 21.31159266242423 28.09743963665768 29.186244727582668 21.404722973335428 17 21.09992494912584 29.186793231613155 28.574465517078668 21.138816302182338 18 20.857762448666573 27.311519151565484 31.34552686424531 20.48519153552263 19 23.32227190840127 26.7637310429677 28.049280212665455 21.864716835965574 20 23.366650093552828 27.613158688144495 28.81248258457131 20.207708633731368 21 22.412380084816057 26.934115068906838 27.575252148464855 23.078252697812246 22 22.88586720311937 28.91673806951533 27.638093109314653 20.55930161805064 23 21.525363283438566 28.486507750444044 28.607999586748946 21.38012937936844 24 22.8948995785453 26.945285394620182 29.311690214651325 20.848124812183194 25 22.229396773140866 27.85511667723809 28.815833196327006 21.099653353294038 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 349.0 1 349.0 2 484.0 3 619.0 4 619.0 5 619.0 6 1456.0 7 2293.0 8 2293.0 9 2293.0 10 3321.0 11 4349.0 12 4349.0 13 4349.0 14 7012.0 15 9675.0 16 9675.0 17 9675.0 18 17725.0 19 25775.0 20 25775.0 21 25775.0 22 41162.0 23 56549.0 24 56549.0 25 56549.0 26 83103.5 27 109658.0 28 109658.0 29 109658.0 30 141333.0 31 173008.0 32 173008.0 33 173008.0 34 192623.0 35 212238.0 36 212238.0 37 212238.0 38 223928.5 39 235619.0 40 235619.0 41 235619.0 42 243547.5 43 251476.0 44 251476.0 45 251476.0 46 253764.5 47 256053.0 48 256053.0 49 256053.0 50 244022.0 51 231991.0 52 231991.0 53 231991.0 54 203090.0 55 174189.0 56 174189.0 57 174189.0 58 148312.5 59 122436.0 60 122436.0 61 122436.0 62 99601.0 63 76766.0 64 76766.0 65 76766.0 66 58585.0 67 40404.0 68 40404.0 69 40404.0 70 29531.5 71 18659.0 72 18659.0 73 18659.0 74 13075.0 75 7491.0 76 7491.0 77 7491.0 78 5053.0 79 2615.0 80 2615.0 81 2615.0 82 1706.0 83 797.0 84 797.0 85 797.0 86 505.5 87 214.0 88 214.0 89 214.0 90 136.0 91 58.0 92 58.0 93 58.0 94 39.0 95 20.0 96 20.0 97 20.0 98 13.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.464340279778355 3 0.0 4 0.0 5 5.960339898316601E-4 6 6.457034889842985E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.9867799661055336E-4 14 0.0 15 0.0 16 0.0 17 3.476864940684684E-4 18 0.0 19 2.4834749576319173E-4 20 3.476864940684684E-4 21 4.966949915263835E-4 22 0.004122568429668983 23 1.9867799661055336E-4 24 0.0021357884635634485 25 9.437204839001285E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2013308.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.39055051469455 #Duplication Level Percentage of deduplicated Percentage of total 1 85.41527505173279 62.686740584107255 2 10.32537723098234 15.15570238507373 3 2.3637040690076137 5.204206286348771 4 0.7868940447679813 2.3100234856902744 5 0.3420758889224251 1.2552568902910142 6 0.1857791961755951 0.8180662488902618 7 0.12460305299574251 0.6401280658618443 8 0.07545363211871323 0.4430066879620481 9 0.05661896519090043 0.37397673224392597 >10 0.2830794983648316 3.6535827904214893 >50 0.02063441473413622 1.0714206704262217 >100 0.016803236325645798 2.550785417357543 >500 0.0020565103785145813 1.0978030817485334 >1k 0.0016452083028116505 2.739300673577082 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 4788 0.23781756194283238 No Hit GTATCAACGCAGAGTACTTTTTTTT 4732 0.23503606999028462 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4567 0.2268406026300993 No Hit CTGTAGGACGTGGAATATGGCAAGA 4536 0.22530084815636753 No Hit CTTTAGGACGTGAAATATGGCGAGG 3984 0.19788328462411117 No Hit GGTATCAACGCAGAGTACTTTTTTT 3111 0.15452181186385788 No Hit TATCAACGCAGAGTACTTTTTTTTT 2897 0.14389253904519328 No Hit GTCCTACAGTGTGCATTTCTCATTT 2737 0.13594541918077113 No Hit CTGTAGGACCTGGAATATGGCGAGA 2179 0.10822983865359896 No Hit CTGAAGGACCTGGAATATGGCGAGA 2129 0.10574636369596703 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.9669499152638346E-5 0.0 5 0.0 4.9669499152638346E-5 0.0 4.9669499152638346E-5 0.0 6 0.0 4.9669499152638346E-5 0.0 4.9669499152638346E-5 0.0 7 0.0 4.9669499152638346E-5 0.0 9.933899830527669E-5 0.0 8 0.0 4.9669499152638346E-5 0.0 9.933899830527669E-5 0.0 9 0.0 4.9669499152638346E-5 0.0 9.933899830527669E-5 0.0 10 0.0 4.9669499152638346E-5 0.0 9.933899830527669E-5 0.0 11 0.0 4.9669499152638346E-5 0.0 9.933899830527669E-5 0.0 12 0.0 4.9669499152638346E-5 0.0 9.933899830527669E-5 0.0 13 0.0 4.9669499152638346E-5 0.0 9.933899830527669E-5 4.9669499152638346E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGTA 40 0.005486681 14.167672 7 TGGACCG 55 2.0584816E-4 13.739032 5 GGACCGT 55 2.0589153E-4 13.73869 6 GGTATCA 970 0.0 13.493694 1 GCGACTA 65 5.7559842E-5 13.078501 16 TAAGCGA 60 4.2979285E-4 12.593487 13 TAGGACC 835 0.0 11.877681 4 TAATACT 240 0.0 11.80698 4 GGCGAGG 1355 0.0 11.71113 19 GTCTTAC 140 3.792593E-9 11.418873 1 AAATGTC 765 0.0 10.988434 7 AATGTCC 780 0.0 10.898209 8 CCACCTT 775 0.0 10.846647 13 GTGGTAT 370 0.0 10.801637 1 ATAGTAC 195 1.8189894E-12 10.656557 3 TCCAACG 160 6.020855E-10 10.626282 18 AGAGCGC 80 3.9790955E-4 10.625754 8 GTACTAA 170 6.493792E-10 10.57925 1 CGGTAGG 85 4.1433342E-4 10.57925 1 TGGCGAG 2860 0.0 10.502433 18 >>END_MODULE