FastQCFastQC Report
Thu 26 May 2016
SRR1511830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013308
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61260.3042753518090625No Hit
GTCCTACAGTGGACATTTCTAAATT54530.2708477788793369No Hit
CTGTAGGACGTGGAATATGGCAAGA52120.25887742958355103No Hit
GTCCTAAAGTGTGTATTTCTCATTT50680.25172502170557115No Hit
CTTTAGGACGTGAAATATGGCGAGG48140.239108968920801No Hit
GGTATCAACGCAGAGTACTTTTTTT41120.20424098051564887No Hit
TATCAACGCAGAGTACTTTTTTTTT39860.19798262362241645No Hit
GTCCTACAGTGTGCATTTCTCATTT31180.15486949835792635No Hit
CTGAAGGACCTGGAATATGGCGAGA25220.1252664768629539No Hit
CTGTAGGACCTGGAATATGGCGAGA24370.12104456943497965No Hit
TTTCTAAATTTTCCACCTTTTTCAG20410.10137544777053485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGAT250.00604184518.9950087
GGTATCA12250.013.6267481
TAGGACC10050.012.7594254
AGTGCGC604.1020414E-412.6633398
TCCAACG1600.012.46640318
GGCGAGG13850.012.00164919
CCAACGT658.036236E-411.69039719
ACCTTTT7600.011.4969815
GACGTGA17000.011.2294037
AGGACCT19050.011.0693325
CCACCTT8100.011.02179413
AGGACGT30850.010.8072555
GGACGTG30400.010.7471766
CCAACGA1158.838124E-710.73737619
CACCTTT8000.010.68469214
GCCGGTT803.7808868E-410.68469111
GCCGTGC803.7808868E-410.6846918
TTAGGAC19150.010.6643183
GGACCTG19150.010.61339956
ACTATCG1451.44973455E-810.48000411
Produced by FastQC (version 0.11.5)