FastQCFastQC Report
Thu 26 May 2016
SRR1511829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086313
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51610.24737419553058435No Hit
GTCCTACAGTGGACATTTCTAAATT42260.2025582930269811No Hit
CTGTAGGACGTGGAATATGGCAAGA38660.18530297227693063No Hit
GTCCTAAAGTGTGTATTTCTCATTT38300.1835774402019256No Hit
CTTTAGGACGTGAAATATGGCGAGG33580.16095379744074834No Hit
GGTATCAACGCAGAGTACTTTTTTT33450.1603306886358854No Hit
TATCAACGCAGAGTACTTTTTTTTT32500.1557772012157332No Hit
GTCCTACAGTGTGCATTTCTCATTT25400.12174587418091148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10250.014.0402841
CGGTAGG758.775774E-613.3252691
GTCGGTC500.001566929313.222980512
TAGGACC9350.012.7283084
CTACGCC550.003204879612.0211794
TGGTATC3250.011.6852362
GGACCTG17100.011.1572356
CTTAGAC1451.3405952E-911.0736983
CGACAGG600.006136346611.01915114
GGCGAGG10550.010.83289819
TAGACCG700.001565493310.7945285
GTAGGAC27500.010.7160233
TGTAGGA27200.010.6920592
AGGACCT18150.010.6681475
GGACGTG24550.010.5414526
CTGTAGG27250.010.452391
AGGACGT25300.010.4158675
TTAGGAC14750.010.3737253
GTATCAA22750.010.36735251
TAGGACA7150.010.3038684
Produced by FastQC (version 0.11.5)