FastQCFastQC Report
Thu 26 May 2016
SRR1511829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086313
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66580.3191275709828775No Hit
GTCCTACAGTGGACATTTCTAAATT46610.2234084722666254No Hit
TATCAACGCAGAGTACTTTTTTTTT44150.21161733642075758No Hit
CTGTAGGACGTGGAATATGGCAAGA43810.20998766723880838No Hit
GGTATCAACGCAGAGTACTTTTTTT43620.2090769697547779No Hit
GTCCTAAAGTGTGTATTTCTCATTT42340.20294174459920444No Hit
CTTTAGGACGTGAAATATGGCGAGG38690.18544676661651438No Hit
GTCCTACAGTGTGCATTTCTCATTT26970.1292711112857946No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21770.10434675909127729No Hit
CTGTAGGACCTGGAATATGGCGAGA21030.10079983204821137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT400.005280170614.2498924
GGTATCA11750.012.9883811
CCGTGCG550.003075844112.087349
GTATTAA2800.011.922931
TAGGACC9850.011.3806264
CCAACGT855.3367752E-511.17397619
GGCGAGG11500.010.90191319
ACTGTTC5300.010.57235058
TGGTATC3900.010.4742792
GATATAC4400.010.4054641
TGTAGGA26650.010.3376322
AGGACGT25100.010.2947445
TAGGACT2600.010.2306924
CTGTAGG26500.010.2222241
GTATCAA26450.010.1694251
GCCGGTT750.002654713810.130342511
GGACGTG25000.010.1050166
GTCCTAC25300.010.1038581
GTGGTAT4450.010.0742051
TGGCGAG24250.010.06579318
Produced by FastQC (version 0.11.5)