##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511829_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2086313 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.272118804800623 31.0 31.0 34.0 28.0 34.0 2 31.459729196913408 33.0 31.0 34.0 28.0 34.0 3 31.57782557075568 34.0 31.0 34.0 28.0 34.0 4 35.143617951860534 37.0 35.0 37.0 32.0 37.0 5 34.829383702253686 37.0 35.0 37.0 32.0 37.0 6 34.782771808448686 37.0 35.0 37.0 32.0 37.0 7 34.6414531280781 37.0 35.0 37.0 32.0 37.0 8 34.56663501593481 37.0 35.0 37.0 31.0 37.0 9 36.135225155573494 39.0 37.0 39.0 32.0 39.0 10 35.9598238615203 39.0 37.0 39.0 31.0 39.0 11 35.947095665894814 39.0 37.0 39.0 31.0 39.0 12 35.79719965316805 39.0 35.0 39.0 30.0 39.0 13 35.7937159956344 39.0 35.0 39.0 30.0 39.0 14 36.89389607407901 40.0 37.0 41.0 31.0 41.0 15 36.84379620891017 40.0 37.0 41.0 31.0 41.0 16 36.763210985120644 40.0 37.0 41.0 30.0 41.0 17 36.713897674989326 40.0 37.0 41.0 30.0 41.0 18 36.65193429749036 40.0 36.0 41.0 30.0 41.0 19 36.6705978441394 40.0 37.0 41.0 30.0 41.0 20 36.550756765643506 40.0 36.0 41.0 30.0 41.0 21 36.447154861231276 40.0 36.0 41.0 30.0 41.0 22 36.538651199508415 40.0 37.0 41.0 30.0 41.0 23 36.441635555163586 40.0 37.0 41.0 30.0 41.0 24 36.34657982766728 40.0 37.0 41.0 30.0 41.0 25 35.936452967507755 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44113482931035364 1101 2 0.37285826820502166 1101 3 0.4573202805742902 1101 4 0.37758193826043396 1101 5 0.5194511896368468 1101 6 0.543185992011999 1101 7 0.49793227830564746 1101 8 0.5170940116677372 1101 9 0.7496158976728253 1101 10 0.73967054248903 1101 11 0.4719182569528684 1101 12 0.6320437502297338 1101 13 0.6925781952211523 1101 14 0.589445621345071 1101 15 0.4708274210585728 1101 16 0.6393898658155379 1101 17 0.7320212965183401 1101 18 0.6110779761640472 1101 19 0.6350774432279493 1101 20 0.7484131317091283 1101 21 0.6824870685677666 1101 22 0.41632387060874976 1101 23 0.26580329042787554 1101 24 0.3452225267076017 1101 25 0.4136132149027887 1102 1 0.4517107195854919 1102 2 0.5096755862253488 1102 3 0.47931895858800644 1102 4 0.3915206311464914 1102 5 0.5277383911571292 1102 6 0.600043795862284 1102 7 0.5190262219559827 1102 8 0.4545971100730881 1102 9 0.6842395447024856 1102 10 0.6605083512967624 1102 11 0.6382571811865247 1102 12 0.9686297206502843 1102 13 0.9328037591309268 1102 14 1.1735900483466395 1102 15 1.1784247109866897 1102 16 1.0726832641465336 1102 17 1.2091096217269666 1102 18 1.032556122078283 1102 19 1.1417782612069587 1102 20 1.1638762400293814 1102 21 0.9692672057238454 1102 22 0.7382564493082455 1102 23 0.9832906902461005 1102 24 0.970918635110479 1102 25 1.2064620953456497 1103 1 0.3929566916585294 1103 2 0.3734135024444676 1103 3 0.41537696073623565 1103 4 0.46558078152243354 1103 5 0.47126148460503714 1103 6 0.5175411105776391 1103 7 0.5070820758764967 1103 8 0.437024607387805 1103 9 0.6882567305241878 1103 10 0.8356103921130895 1103 11 0.6484827450963024 1103 12 0.7686082383731687 1103 13 0.6146082704091214 1103 14 0.8319036895926146 1103 15 0.773279705616126 1103 16 0.6912869161336417 1103 17 0.6871976990631623 1103 18 0.8068848009182403 1103 19 0.9611445340318809 1103 20 0.84196431736558 1103 21 0.8656044774746547 1103 22 0.9979664385671043 1103 23 1.0558900457777867 1103 24 0.9616597736711654 1103 25 1.0185351350878662 1104 1 0.5004461545354353 1104 2 0.5209526789277703 1104 3 0.4381684878085359 1104 4 0.5186068271292612 1104 5 0.5145760852750882 1104 6 0.5908449039419565 1104 7 0.5027656379644725 1104 8 0.6036386784998768 1104 9 0.7444147333340823 1104 10 0.5760448458037857 1104 11 0.583908012577119 1104 12 0.6993723148888904 1104 13 0.7129947396623351 1104 14 0.8475937360640273 1104 15 0.822395668006699 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2314 22 -0.6874822672310046 2314 23 -0.7235078186311341 2314 24 -0.8319498807169055 2314 25 -0.7062608885847652 2315 1 -0.5792883087598426 2315 2 -0.554892915428514 2315 3 -0.7099782396654071 2315 4 -0.6261855978600437 2315 5 -0.6377729058225441 2315 6 -0.7232328832304091 2315 7 -0.6608729069701269 2315 8 -0.7657149123176623 2315 9 -0.9618177402314032 2315 10 -1.1346992054101293 2315 11 -0.9639279318344975 2315 12 -0.9488739806879991 2315 13 -0.9090684093666326 2315 14 -1.1673382148505311 2315 15 -1.2333314566297133 2315 16 -1.168416874344139 2315 17 -1.3377458203076031 2315 18 -1.324539347688571 2315 19 -1.3529237447732427 2315 20 -1.2813559068834408 2315 21 -1.3600285239478254 2315 22 -1.3704203073118961 2315 23 -1.2874820569751222 2315 24 -1.2744485017869565 2315 25 -1.1325476194933444 2316 1 -1.82254038436486 2316 2 -1.3710977757510214 2316 3 -1.0801034068493962 2316 4 -1.023516962838471 2316 5 -1.2548162096305546 2316 6 -1.0563561325301265 2316 7 -1.1665060611326865 2316 8 -1.1782051336314083 2316 9 -1.4435770205200953 2316 10 -1.3706469178284308 2316 11 -1.1767386417040342 2316 12 -1.3467718785858978 2316 13 -1.419723392080435 2316 14 -1.6245958926964406 2316 15 -1.4879943127631563 2316 16 -1.4421424907168188 2316 17 -1.4619042224071777 2316 18 -1.530771840685773 2316 19 -1.3503926422421415 2316 20 -1.4739922406962407 2316 21 -1.6603395492588504 2316 22 -1.5537921245072468 2316 23 -1.5660648414402587 2316 24 -1.468757937272862 2316 25 -1.5065943553047845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23003.0 3 0.0 4 399.0 5 3809.0 6 4929.0 7 4128.0 8 3386.0 9 2982.0 10 2832.0 11 2794.0 12 2554.0 13 2509.0 14 2501.0 15 2583.0 16 2643.0 17 2753.0 18 2996.0 19 3488.0 20 4141.0 21 5267.0 22 6879.0 23 8944.0 24 10750.0 25 13070.0 26 15375.0 27 17299.0 28 20462.0 29 25311.0 30 32578.0 31 42615.0 32 54429.0 33 72211.0 34 95308.0 35 129384.0 36 191639.0 37 348373.0 38 921989.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18185091357709 19.64473526505264 13.129815599860024 25.043598221510255 2 14.79437649096756 20.90386245975556 41.19559241590308 23.1061686333738 3 18.40792824470729 25.440478010729933 30.32229583959837 25.829297904964406 4 12.402741103564038 17.49996285312894 38.6325062442692 31.464789799037824 5 12.728405106551094 38.28192085871834 36.16202229165758 12.827651743072993 6 31.06427463185054 37.98552757903536 18.25047344286308 12.699724346251017 7 27.359701061154297 31.782143906499165 22.940613417066373 17.91754161528016 8 24.561559075747503 37.65532784390454 20.82851422581367 16.954598854534293 9 26.327353565836 14.97665978211323 20.545143513940623 38.150843138110154 10 14.680059990998473 29.213977001533326 35.115680149622804 20.990282857845397 11 33.887532695237965 22.191109387709325 25.468469975502238 18.45288794155048 12 23.278050800622918 24.839609397055952 32.347111866723736 19.53522793559739 13 28.98069465128195 22.311033866922173 26.06334715836023 22.64492432343565 14 20.458243801385507 22.61563820960709 28.1860391992956 28.740078789711802 15 22.423337246137084 31.90317080898216 23.64515774958024 22.028334195300513 16 21.535475775184047 28.342754268179576 28.570668514905016 21.55110144173136 17 21.208514733887004 29.03787686698976 28.370335611195447 21.383272787927794 18 20.982863541023587 27.438561442392125 30.839970244143732 20.738604772440556 19 23.18405723398167 26.93004357447804 28.06232813580704 21.823571055733247 20 23.414080245869147 27.432173408304507 28.696940487836674 20.456805857989668 21 22.523993283845712 26.777094328607454 27.729348376777597 22.969564010769236 22 22.90988935984198 28.40570901873305 27.848505952846004 20.83589566857897 23 21.75239285763929 28.158718274774685 28.50070914575138 21.588179721834642 24 22.798084177689066 26.87936857625757 29.177272043125175 21.145275202928186 25 22.28918781083558 27.673425642952733 28.717708568996393 21.319677977215296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 321.0 1 321.0 2 471.5 3 622.0 4 622.0 5 622.0 6 1640.5 7 2659.0 8 2659.0 9 2659.0 10 3749.0 11 4839.0 12 4839.0 13 4839.0 14 7510.0 15 10181.0 16 10181.0 17 10181.0 18 18318.0 19 26455.0 20 26455.0 21 26455.0 22 42293.5 23 58132.0 24 58132.0 25 58132.0 26 84100.0 27 110068.0 28 110068.0 29 110068.0 30 140874.5 31 171681.0 32 171681.0 33 171681.0 34 192248.5 35 212816.0 36 212816.0 37 212816.0 38 226184.5 39 239553.0 40 239553.0 41 239553.0 42 249923.5 43 260294.0 44 260294.0 45 260294.0 46 263862.0 47 267430.0 48 267430.0 49 267430.0 50 256260.0 51 245090.0 52 245090.0 53 245090.0 54 216240.5 55 187391.0 56 187391.0 57 187391.0 58 159225.0 59 131059.0 60 131059.0 61 131059.0 62 106532.0 63 82005.0 64 82005.0 65 82005.0 66 63066.0 67 44127.0 68 44127.0 69 44127.0 70 32069.5 71 20012.0 72 20012.0 73 20012.0 74 13876.5 75 7741.0 76 7741.0 77 7741.0 78 5241.0 79 2741.0 80 2741.0 81 2741.0 82 1781.5 83 822.0 84 822.0 85 822.0 86 516.0 87 210.0 88 210.0 89 210.0 90 130.5 91 51.0 92 51.0 93 51.0 94 31.5 95 12.0 96 12.0 97 12.0 98 6.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.422755358376236 2 0.0 3 0.0 4 0.0 5 0.028758867916750745 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 9.586289305583581E-5 17 0.0 18 4.7931446527917905E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005128664778487216 25 4.7931446527917905E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2086313.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.07513514430887 #Duplication Level Percentage of deduplicated Percentage of total 1 84.2556378530569 60.72736484929023 2 10.999402332175137 15.855668191962986 3 2.608035276966192 5.6392348514538995 4 0.868390122275579 2.503573416839811 5 0.3984817780764532 1.436031400370243 6 0.23281811597287005 1.0068238303672794 7 0.12887684638521407 0.6502171284130648 8 0.08707765884319414 0.5020907223338592 9 0.06720026664160053 0.4359121469934255 >10 0.314211256203195 3.950496252610758 >50 0.01980398773628766 1.0038755661306318 >100 0.0164790916190459 2.417496285364422 >500 0.0021246898063465676 1.163221397183153 >1k 0.0013943276854148986 2.3893726556118224 >5k 6.63965564483285E-5 0.31862130507447606 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6658 0.3191275709828775 No Hit GTCCTACAGTGGACATTTCTAAATT 4661 0.2234084722666254 No Hit TATCAACGCAGAGTACTTTTTTTTT 4415 0.21161733642075758 No Hit CTGTAGGACGTGGAATATGGCAAGA 4381 0.20998766723880838 No Hit GGTATCAACGCAGAGTACTTTTTTT 4362 0.2090769697547779 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4234 0.20294174459920444 No Hit CTTTAGGACGTGAAATATGGCGAGG 3869 0.18544676661651438 No Hit GTCCTACAGTGTGCATTTCTCATTT 2697 0.1292711112857946 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2177 0.10434675909127729 No Hit CTGTAGGACCTGGAATATGGCGAGA 2103 0.10079983204821137 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 4.793144652791791E-5 0.0 0.0 0.0 4 0.0 4.793144652791791E-5 0.0 0.0 0.0 5 0.0 4.793144652791791E-5 0.0 0.0 0.0 6 0.0 4.793144652791791E-5 0.0 0.0 0.0 7 0.0 4.793144652791791E-5 0.0 0.0 0.0 8 0.0 4.793144652791791E-5 0.0 0.0 0.0 9 0.0 4.793144652791791E-5 0.0 9.586289305583582E-5 0.0 10 0.0 4.793144652791791E-5 0.0 1.4379433958375373E-4 0.0 11 0.0 4.793144652791791E-5 0.0 1.4379433958375373E-4 0.0 12 0.0 4.793144652791791E-5 0.0 1.4379433958375373E-4 9.586289305583582E-5 13 0.0 4.793144652791791E-5 0.0 2.3965723263958956E-4 9.586289305583582E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACGT 40 0.0052801706 14.249892 4 GGTATCA 1175 0.0 12.988381 1 CCGTGCG 55 0.0030758441 12.08734 9 GTATTAA 280 0.0 11.92293 1 TAGGACC 985 0.0 11.380626 4 CCAACGT 85 5.3367752E-5 11.173976 19 GGCGAGG 1150 0.0 10.901913 19 ACTGTTC 530 0.0 10.5723505 8 TGGTATC 390 0.0 10.474279 2 GATATAC 440 0.0 10.405464 1 TGTAGGA 2665 0.0 10.337632 2 AGGACGT 2510 0.0 10.294744 5 TAGGACT 260 0.0 10.230692 4 CTGTAGG 2650 0.0 10.222224 1 GTATCAA 2645 0.0 10.169425 1 GCCGGTT 75 0.0026547138 10.1303425 11 GGACGTG 2500 0.0 10.105016 6 GTCCTAC 2530 0.0 10.103858 1 GTGGTAT 445 0.0 10.074205 1 TGGCGAG 2425 0.0 10.065793 18 >>END_MODULE