FastQCFastQC Report
Thu 26 May 2016
SRR1511828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399278
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55820.23265332320806512No Hit
GTCCTACAGTGGACATTTCTAAATT51990.2166901876314458No Hit
CTGTAGGACGTGGAATATGGCAAGA47460.1978095076935645No Hit
GTCCTAAAGTGTGTATTTCTCATTT42480.1770532635234433No Hit
CTTTAGGACGTGAAATATGGCGAGG38840.16188203284488084No Hit
GGTATCAACGCAGAGTACTTTTTTT35030.1460022556785833No Hit
TATCAACGCAGAGTACTTTTTTTTT34500.14379325780505636No Hit
GTCCTACAGTGTGCATTTCTCATTT28850.12024450688915582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11750.013.0975971
TAGGACC10250.012.6242484
CTAGTAC1201.0921212E-811.8064363
GTACTAG1251.1143857E-711.1924921
GGCGAGG12250.011.02599519
GTATCAA27050.010.9723071
AGGACCT19650.010.8631235
GTGCGCC1053.717234E-610.79445611
AAATGTC8500.010.7783567
GGACCTG18550.010.692626
TACCGTA803.9803295E-410.625577
AATGTCC8600.010.5432018
GTCCTAC27500.010.5383851
TGTCGAG1356.194023E-810.49482818
GTCCTAA14600.010.403981
TGTAGGA30200.010.3903812
GCCCTAA1551.8542778E-810.3156611
TAGAAAT9350.010.1017644
GGACGTG28750.010.0529236
ATAAGCC2350.010.048033
Produced by FastQC (version 0.11.5)