FastQCFastQC Report
Thu 26 May 2016
SRR1511828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399278
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74530.3106351160640826No Hit
GTCCTACAGTGGACATTTCTAAATT57020.2376548278273714No Hit
CTGTAGGACGTGGAATATGGCAAGA53140.22148329622494767No Hit
GGTATCAACGCAGAGTACTTTTTTT47900.19964339272064346No Hit
GTCCTAAAGTGTGTATTTCTCATTT46660.1944751712806936No Hit
TATCAACGCAGAGTACTTTTTTTTT46450.193599907972315No Hit
CTTTAGGACGTGAAATATGGCGAGG43490.1812628632446928No Hit
GTCCTACAGTGTGCATTTCTCATTT33000.13754137703092345No Hit
CTGAAGGACCTGGAATATGGCGAGA24080.10036352602741325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTTA350.002174961416.28089113
TCCAACG1950.013.15022518
TAGGACC10050.013.1392464
GGTATCA14050.012.8322691
GGACGAT550.003076098212.0873286
GGCGAGG12350.011.84289319
GCCGGTT1251.841363E-811.39662411
AGGACCT19450.010.8919835
TGTAGGA32500.010.7568772
GACCGGG803.782624E-410.6843357
CTAATAC1701.382432E-1010.61763
GGACCTG19400.010.5741876
GGACCGG909.547733E-510.5524296
TGGTATC4800.010.4895372
GTATCAA29700.010.4707811
ACAGCGC1002.4077222E-510.4469048
GTCCTAA13850.010.4002821
GTCCTAC31200.010.3954261
ATTTAGA8000.010.3740231
TGGCGAG30600.010.27331518
Produced by FastQC (version 0.11.5)