FastQCFastQC Report
Thu 26 May 2016
SRR1511827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096608
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52600.25088142370915306No Hit
GGTATCAACGCAGAGTACTTTTTTT33050.15763557136097925No Hit
TATCAACGCAGAGTACTTTTTTTTT32880.15682473786229947No Hit
GTCCTACAGTGGACATTTCTAAATT29470.1405603718005464No Hit
CTGTAGGACGTGGAATATGGCAAGA28650.13664929257162045No Hit
GTCCTAAAGTGTGTATTTCTCATTT28130.1341690959874235No Hit
CTTTAGGACGTGAAATATGGCGAGG24360.11618767075199561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA250.006237271218.89063510
GGTATCA11600.013.347611
CGTAAGA550.002105777612.7135022
GCCGGTT1353.092282E-1112.59375511
TAAGACG550.003204625312.0213124
TAGGACC3650.011.6449114
TGCCGGT1302.8649083E-911.62500410
GTATCAA25050.011.00604251
TCCAACG1553.45608E-1010.97006218
GGCGAGG8400.010.68347619
GTCTAAG1956.184564E-1110.2453131
TTAGGAC11000.010.1322493
GTCCTAA9700.09.9891811
AGGACGT17700.09.978955
GGACGTG17350.09.9080556
TGGCGAG16250.09.88243218
GTAGGAC14550.09.67252353
ATTATAC2253.6379788E-129.6552123
GTGGTAT3850.09.5999921
TGTAGGA13600.09.548482
Produced by FastQC (version 0.11.5)