FastQCFastQC Report
Thu 26 May 2016
SRR1511827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096608
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66540.3173697706008944No Hit
GGTATCAACGCAGAGTACTTTTTTT43640.2081457287199133No Hit
TATCAACGCAGAGTACTTTTTTTTT42690.20361460034493808No Hit
GTCCTAAAGTGTGTATTTCTCATTT31570.15057655031364947No Hit
GTCCTACAGTGGACATTTCTAAATT31000.14785787328866437No Hit
CTGTAGGACGTGGAATATGGCAAGA30660.1462362062913048No Hit
CTTTAGGACGTGAAATATGGCGAGG26440.1261084570887834No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21480.10245119736259711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG350.00216975716.2865925
GGTATCA12500.014.8768771
CGAAAAA500.001503018913.29627715
GATCGCA500.00150328913.2959617
CGTGCGC550.003075500712.08752510
AAGGCGT1104.9903065E-711.2238636
GGCGAGG8850.010.73145919
GTATTAG1605.0931703E-1010.7285171
AGGACGT19000.010.6005735
TTAGGAC12400.010.5731513
GTATCAA26950.010.4741821
CTTACAC3100.010.4199173
GTCCTAA8500.010.2096211
TAGGACC4950.010.1722664
GGACGTG19550.010.1043466
GTCCATA1701.4588295E-910.0974271
TCCTACA16800.010.0094692
TAGGACG20200.09.9708364
GTAATAC1151.0260277E-59.9135793
TAGACTG2600.09.8659175
Produced by FastQC (version 0.11.5)