##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511827_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2096608 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.919831461102888 31.0 31.0 34.0 28.0 34.0 2 31.106907919840047 31.0 31.0 34.0 27.0 34.0 3 31.220806178360476 31.0 31.0 34.0 27.0 34.0 4 34.81867378165112 37.0 35.0 37.0 32.0 37.0 5 34.45460047848716 37.0 35.0 37.0 30.0 37.0 6 34.38946002304675 37.0 35.0 37.0 30.0 37.0 7 34.222446446832215 37.0 35.0 37.0 30.0 37.0 8 34.13328814923915 37.0 35.0 37.0 30.0 37.0 9 35.64576163021414 39.0 35.0 39.0 30.0 39.0 10 35.4501413712053 39.0 35.0 39.0 30.0 39.0 11 35.44176307635953 39.0 35.0 39.0 30.0 39.0 12 35.261947393122604 39.0 35.0 39.0 29.0 39.0 13 35.24596157221569 39.0 35.0 39.0 29.0 39.0 14 36.26349751598773 40.0 36.0 41.0 29.0 41.0 15 36.19496539171843 40.0 36.0 41.0 29.0 41.0 16 36.09579330041667 39.0 36.0 41.0 28.0 41.0 17 36.03263557136098 39.0 36.0 41.0 28.0 41.0 18 35.96206253147942 39.0 36.0 41.0 27.0 41.0 19 35.96343951754453 39.0 36.0 41.0 27.0 41.0 20 35.828113791419284 39.0 36.0 41.0 27.0 41.0 21 35.7145870854256 39.0 36.0 41.0 27.0 41.0 22 35.80070046475068 39.0 36.0 41.0 27.0 41.0 23 35.69521579618126 39.0 36.0 41.0 27.0 41.0 24 35.56322593446176 39.0 36.0 41.0 27.0 41.0 25 35.114105736503916 39.0 35.0 40.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3660079236793443 1101 2 0.2542705452275982 1101 3 0.30487805050015027 1101 4 0.32302048445744447 1101 5 0.4055513227287335 1101 6 0.47898092501262823 1101 7 0.2955554324152061 1101 8 0.2894226689903405 1101 9 0.4009296244922922 1101 10 0.5267967238008637 1101 11 0.22164038609468406 1101 12 0.44501421794968365 1101 13 0.5294621345995267 1101 14 0.3575959233385504 1101 15 0.19762370136940888 1101 16 0.2664614419237097 1101 17 0.43518979083668086 1101 18 0.2424734707815972 1101 19 0.49233316761917223 1101 20 0.3060568505152972 1101 21 0.5221244912786389 1101 22 0.35986863381095446 1101 23 0.18920326856723335 1101 24 0.058636559722877735 1101 25 0.12523005430870882 1102 1 0.4171513874045054 1102 2 0.39272926538566466 1102 3 0.4398610727380756 1102 4 0.4582472144554188 1102 5 0.48315588807840015 1102 6 0.5671548832846298 1102 7 0.7317440720352195 1102 8 0.6502982504541635 1102 9 0.5740275590281314 1102 10 0.751173060922298 1102 11 0.7128693601667919 1102 12 0.7568145804036419 1102 13 0.9548278598018527 1102 14 1.1698730697660267 1102 15 0.9643307679130757 1102 16 0.964345600592182 1102 17 1.0161416577362559 1102 18 1.187161622986089 1102 19 1.2230540846145956 1102 20 1.1788634806748703 1102 21 1.324139741823302 1102 22 0.8917534598134296 1102 23 1.1693247298978449 1102 24 1.1549575539196866 1102 25 1.1861627189995403 1103 1 0.3770502959749109 1103 2 0.44990790710537 1103 3 0.4696133923454653 1103 4 0.42706351177412927 1103 5 0.5436669685170159 1103 6 0.527884505246135 1103 7 0.5878421473361968 1103 8 0.4567739808429323 1103 9 0.6098632566995832 1103 10 0.6207766189316573 1103 11 0.716252919709774 1103 12 0.7340274730754714 1103 13 0.5258570497923998 1103 14 0.8619819315608765 1103 15 0.7721899556031815 1103 16 0.6091197743518606 1103 17 0.7606653394580078 1103 18 0.8194623156732561 1103 19 0.8582431038615894 1103 20 0.8155381988713302 1103 21 0.7699932039811088 1103 22 0.90020756609362 1103 23 0.8045552848143913 1103 24 0.88452519955014 1103 25 0.814856198538429 1104 1 0.3485514856785663 1104 2 0.3471669534883617 1104 3 0.32909084977879743 1104 4 0.3663855351973879 1104 5 0.4097774100979308 1104 6 0.39267082210902515 1104 7 0.4999539485784297 1104 8 0.4231285434337053 1104 9 0.48557476128844 1104 10 0.5198758433066715 1104 11 0.5631411493689313 1104 12 0.6616085119803259 1104 13 0.539526281274739 1104 14 0.7107443307450794 1104 15 0.748255281149369 1104 16 0.9835643218009551 1104 17 0.9669831293903357 1104 18 0.9526704983622949 1104 19 0.9829951201775202 1104 20 0.7520945475626561 1104 21 0.7491950209830591 1104 22 0.9045229457172397 1104 23 0.8370063833589327 1104 24 0.9144671049300683 1104 25 1.0177562943327487 1105 1 0.358856053214172 1105 2 0.41060907490485477 1105 3 0.4771770396545669 1105 4 0.4937218399213066 1105 5 0.4884161417833184 1105 6 0.5116569328128762 1105 7 0.6011654744461481 1105 8 0.6421850291778739 1105 9 0.7160715895244678 1105 10 0.681149533793338 1105 11 0.7327855599290984 1105 12 0.8297628288048458 1105 13 0.7029855077357539 1105 14 0.6521013961058983 1105 15 0.722511700428214 1105 16 0.8351404294747624 1105 17 0.7657070930689969 1105 18 0.8448378541178982 1105 19 0.8009422216055526 1105 20 0.8777374528789039 1105 21 0.7712673704342308 1105 22 0.8688872733912518 1105 23 0.8317175035927349 1105 24 0.9260662799782082 1105 25 0.992871386846609 1106 1 0.16542569138925955 1106 2 0.25087306446296154 1106 3 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1107 15 0.6862032156718527 1107 16 0.7661473222241142 1107 17 0.7334848351814145 1107 18 0.7749849233541113 1107 19 0.886021793633887 1107 20 0.838137423045886 1107 21 0.7129641140090399 1107 22 0.5109002375378111 1107 23 0.7081936952377959 1107 24 0.7322087666359565 1107 25 0.6177218677152396 1108 1 0.03777035416143093 1108 2 0.15567922484532914 1108 3 0.06717875597273348 1108 4 0.20125209459371263 1108 5 0.1288830898774691 1108 6 0.2189918193532847 1108 7 0.21888207745080024 1108 8 0.3351220147388929 1108 9 0.3342200108769262 1108 10 0.14416379018935999 1108 11 0.33462804574208604 1108 12 0.4376206446256461 1108 13 0.3186945662439413 1108 14 0.5742992328146599 1108 15 0.5775951061877436 1108 16 0.4036899945524368 1108 17 0.4382564098282913 1108 18 0.4468492329421849 1108 19 0.46877440220604427 1108 20 0.41943211622491816 1108 21 0.3975253951346076 1108 22 0.42391991995386746 1108 23 0.47263909341460675 1108 24 0.5173671030805806 1108 25 0.4666674079098456 1109 1 0.15008460833293924 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-0.808107396549758 2313 19 -0.6755874317468695 2313 20 -0.681094525658601 2313 21 -0.7589698547621282 2313 22 -0.6939549251514592 2313 23 -0.7066773047679646 2313 24 -0.7713565648252398 2313 25 -0.6503813322801548 2314 1 -0.3571329794346916 2314 2 -0.2927764778054147 2314 3 -0.46268761515591095 2314 4 -0.3598117816441828 2314 5 -0.4022765127835797 2314 6 -0.4044958729637358 2314 7 -0.4282135337489237 2314 8 -0.4500431186474074 2314 9 -0.4218656062052517 2314 10 -0.4148692728742489 2314 11 -0.4769353847630171 2314 12 -0.5128787580317677 2314 13 -0.4418559592355962 2314 14 -0.4188434562579104 2314 15 -0.40347262093888503 2314 16 -0.5662920245261418 2314 17 -0.4843081304604908 2314 18 -0.48220041623275733 2314 19 -0.5888706732046671 2314 20 -0.43520062421305283 2314 21 -0.36378696955365086 2314 22 -0.33300637342927786 2314 23 -0.35959178404223024 2314 24 -0.5612789608259803 2314 25 -0.3110840066095193 2315 1 -0.8105739716161011 2315 2 -0.7829497636141092 2315 3 -0.7578234254657801 2315 4 -0.7250077613711667 2315 5 -0.8405728217456812 2315 6 -0.9207823491049183 2315 7 -1.0558669066758526 2315 8 -1.0834315335589082 2315 9 -1.1949127243912159 2315 10 -1.2561126883370122 2315 11 -1.2573524973307926 2315 12 -1.1152341061713287 2315 13 -1.2352896431500824 2315 14 -1.3624123464114248 2315 15 -1.4040471484267485 2315 16 -1.432991909587635 2315 17 -1.5380270282391635 2315 18 -1.5232391077955043 2315 19 -1.3693711343450872 2315 20 -1.2267846300015677 2315 21 -1.3869065432314827 2315 22 -1.2348709387656456 2315 23 -1.3943095179117222 2315 24 -1.2514741318242386 2315 25 -1.2009635423398137 2316 1 -1.6960799250677816 2316 2 -1.3401384286545905 2316 3 -1.111664814749851 2316 4 -0.9274309212340981 2316 5 -0.9716434250952162 2316 6 -0.9499974424673141 2316 7 -1.2011658110829089 2316 8 -1.1324827894960734 2316 9 -1.4021973572259157 2316 10 -1.3894733549869827 2316 11 -1.1790092212686645 2316 12 -1.3531379115758 2316 13 -1.460046930095693 2316 14 -1.5879685774709031 2316 15 -1.6584885498798414 2316 16 -1.628264693711408 2316 17 -1.683080562800086 2316 18 -1.6358157879448 2316 19 -1.4860486409431317 2316 20 -1.506805340542904 2316 21 -1.7956976806052722 2316 22 -1.678732224560008 2316 23 -1.6264870874272432 2316 24 -1.5769068324294224 2316 25 -1.6757788973858396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30228.0 3 1.0 4 556.0 5 4895.0 6 6422.0 7 5113.0 8 4082.0 9 3816.0 10 3467.0 11 3473.0 12 3233.0 13 3171.0 14 3260.0 15 3068.0 16 3283.0 17 3387.0 18 3757.0 19 4177.0 20 5075.0 21 6562.0 22 8312.0 23 10671.0 24 12808.0 25 15418.0 26 17722.0 27 20315.0 28 23755.0 29 29561.0 30 37622.0 31 48200.0 32 62572.0 33 81289.0 34 106081.0 35 142448.0 36 210638.0 37 375398.0 38 792772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0546004478524 19.053792091516662 13.031489501903085 24.860117958727855 2 15.73327012011783 20.58501159968864 39.57768929623468 24.10402898395885 3 18.944838520123934 24.973671759336984 29.842583830644546 26.238905889894536 4 13.040969031883881 17.19210267250721 37.389297379386136 32.37763091622278 5 13.43976496811154 37.790784215688895 35.368472979729546 13.400977836470018 6 32.147354202597725 37.042689906744606 17.71919214273722 13.09076374792045 7 28.25554419328744 31.51809971153406 22.074608128939698 18.1517479662388 8 25.740052503853843 36.328631770936674 20.12622292770036 17.80509279750912 9 26.73904707031548 14.775914238617805 19.725051130206506 38.75998756086021 10 15.294990766037333 28.78611547795296 34.11596254521589 21.802931210793815 11 34.91739991452861 22.015369587447918 24.24015361956074 18.827076878462737 12 23.60784657885499 24.438760130649126 31.38335826248874 20.570035028007144 13 29.341250248019655 21.579188861246358 25.642084738778063 23.43747615195592 14 21.495577618706026 21.550618904439933 27.46078427631679 29.493019200537248 15 23.43242036661121 30.230782292159525 23.26228842015293 23.07450892107633 16 22.82538810380978 27.697028146469243 26.990695429370543 22.486888320350435 17 22.233865367298037 28.079736412338406 27.269522962804682 22.41687525755888 18 22.128960159801508 27.012006576358594 29.023357329315402 21.8356759345245 19 23.823337505151176 26.49303064759841 27.42572765152093 22.257904195729484 20 24.01097391596331 26.70317961202094 27.715672171431187 21.57017430058456 21 23.619150551748348 26.141033516995073 27.080455669347824 23.15936026190876 22 23.792287351760557 27.144940780536942 27.05980326317557 22.00296860452693 23 22.996001159968866 27.00252026129825 27.39157725240007 22.60990132633282 24 23.4830377546674 26.30887823519956 27.901668917233362 22.306415092899673 25 23.284001245822417 26.741206148791836 27.620197776693484 22.35459482869226 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 309.0 1 309.0 2 491.5 3 674.0 4 674.0 5 674.0 6 1621.0 7 2568.0 8 2568.0 9 2568.0 10 3375.5 11 4183.0 12 4183.0 13 4183.0 14 6402.0 15 8621.0 16 8621.0 17 8621.0 18 15498.5 19 22376.0 20 22376.0 21 22376.0 22 35340.0 23 48304.0 24 48304.0 25 48304.0 26 69494.0 27 90684.0 28 90684.0 29 90684.0 30 117099.0 31 143514.0 32 143514.0 33 143514.0 34 164411.0 35 185308.0 36 185308.0 37 185308.0 38 202653.5 39 219999.0 40 219999.0 41 219999.0 42 236453.5 43 252908.0 44 252908.0 45 252908.0 46 262940.5 47 272973.0 48 272973.0 49 272973.0 50 269349.0 51 265725.0 52 265725.0 53 265725.0 54 241130.0 55 216535.0 56 216535.0 57 216535.0 58 187319.5 59 158104.0 60 158104.0 61 158104.0 62 130971.5 63 103839.0 64 103839.0 65 103839.0 66 80391.0 67 56943.0 68 56943.0 69 56943.0 70 41871.5 71 26800.0 72 26800.0 73 26800.0 74 18788.0 75 10776.0 76 10776.0 77 10776.0 78 7280.5 79 3785.0 80 3785.0 81 3785.0 82 2527.5 83 1270.0 84 1270.0 85 1270.0 86 799.0 87 328.0 88 328.0 89 328.0 90 196.5 91 65.0 92 65.0 93 65.0 94 41.0 95 17.0 96 17.0 97 17.0 98 8.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4016010622872755 2 0.0 3 0.0 4 0.0 5 0.026328240663013782 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.9078435263053463E-4 17 0.0 18 3.3387261710343566E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004721912727605733 25 4.769608815763366E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2096608.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.47489960655979 #Duplication Level Percentage of deduplicated Percentage of total 1 80.39831784509455 55.05266742979135 2 12.83211554712365 17.573556476581338 3 3.5869866344388934 7.36855649049825 4 1.328971356568956 3.6400472088421134 5 0.6206666974903362 2.1250044899892897 6 0.3482709410273197 1.4308690633636694 7 0.19604899371444248 0.9397104613794462 8 0.14801120958239836 0.8108042173440166 9 0.10583077441472151 0.6522076487999278 >10 0.3972695939875052 4.529740051165083 >50 0.01904243073913486 0.8977384743224903 >100 0.015055370528435034 1.9852084175069424 >500 0.002158995183204648 0.9882663560703114 >1k 0.001183965100466898 1.688298293567404 >5k 6.964500590981753E-5 0.3173249207784167 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6654 0.3173697706008944 No Hit GGTATCAACGCAGAGTACTTTTTTT 4364 0.2081457287199133 No Hit TATCAACGCAGAGTACTTTTTTTTT 4269 0.20361460034493808 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3157 0.15057655031364947 No Hit GTCCTACAGTGGACATTTCTAAATT 3100 0.14785787328866437 No Hit CTGTAGGACGTGGAATATGGCAAGA 3066 0.1462362062913048 No Hit CTTTAGGACGTGAAATATGGCGAGG 2644 0.1261084570887834 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2148 0.10245119736259711 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.7696088157633664E-5 0.0 8 0.0 0.0 0.0 4.7696088157633664E-5 0.0 9 0.0 0.0 0.0 4.7696088157633664E-5 4.7696088157633664E-5 10 0.0 0.0 0.0 4.7696088157633664E-5 4.7696088157633664E-5 11 0.0 0.0 0.0 4.7696088157633664E-5 4.7696088157633664E-5 12 0.0 0.0 0.0 4.7696088157633664E-5 9.539217631526733E-5 13 0.0 0.0 0.0 4.7696088157633664E-5 9.539217631526733E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACGG 35 0.002169757 16.286592 5 GGTATCA 1250 0.0 14.876877 1 CGAAAAA 50 0.0015030189 13.296277 15 GATCGCA 50 0.001503289 13.295961 7 CGTGCGC 55 0.0030755007 12.087525 10 AAGGCGT 110 4.9903065E-7 11.223863 6 GGCGAGG 885 0.0 10.731459 19 GTATTAG 160 5.0931703E-10 10.728517 1 AGGACGT 1900 0.0 10.600573 5 TTAGGAC 1240 0.0 10.573151 3 GTATCAA 2695 0.0 10.474182 1 CTTACAC 310 0.0 10.419917 3 GTCCTAA 850 0.0 10.209621 1 TAGGACC 495 0.0 10.172266 4 GGACGTG 1955 0.0 10.104346 6 GTCCATA 170 1.4588295E-9 10.097427 1 TCCTACA 1680 0.0 10.009469 2 TAGGACG 2020 0.0 9.970836 4 GTAATAC 115 1.0260277E-5 9.913579 3 TAGACTG 260 0.0 9.865917 5 >>END_MODULE