FastQCFastQC Report
Thu 26 May 2016
SRR1511826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412448
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43070.30493157978205215No Hit
GTCCTACAGTGGACATTTCTAAATT28940.20489249869729717No Hit
GGTATCAACGCAGAGTACTTTTTTT28540.20206053603389293No Hit
TATCAACGCAGAGTACTTTTTTTTT26250.18584754978590362No Hit
GTCCTAAAGTGTGTATTTCTCATTT26020.18421917125444617No Hit
CTGTAGGACGTGGAATATGGCAAGA24880.17614807766374407No Hit
CTTTAGGACGTGAAATATGGCGAGG22590.15993509141575477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.015.0713431
CCAACGA1001.0886652E-813.22609619
TTACACC1208.094503E-1012.595394
TAGGACC3850.012.2682384
GGCGAGG5900.012.1692919
TTAAGAC1104.123831E-812.0228723
GTCTTAC1352.1791493E-911.8277611
CACCTTT4750.011.73360114
GTATCAA17200.011.2561441
TCCAACG1201.3766476E-711.02174818
CCACCTT4400.010.94940213
CTTACAC1752.3646862E-1110.7960483
GTACTAG650.007385748410.747341
TGGCGAG14150.010.61559518
GACGTGA8650.010.5928587
CTCGAAC1252.4172732E-710.58087718
CGCCCTA901.0069043E-410.49653116
TTAGGAC9450.010.496163
TTCCACC4700.010.45149411
TAAGACC1002.5519717E-510.3911964
Produced by FastQC (version 0.11.5)