FastQCFastQC Report
Thu 26 May 2016
SRR1511826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412448
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55280.3913772400824667No Hit
TATCAACGCAGAGTACTTTTTTTTT41000.29027617299893516No Hit
GGTATCAACGCAGAGTACTTTTTTT40230.28482464487188197No Hit
GTCCTACAGTGGACATTTCTAAATT25570.18103321325811642No Hit
CTGTAGGACGTGGAATATGGCAAGA25420.1799712272593398No Hit
CTTTAGGACGTGAAATATGGCGAGG25390.1797588300595845No Hit
GTCCTAAAGTGTGTATTTCTCATTT24890.17621887673032918No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19540.1383413761072974No Hit
CTGAAGGACCTGGAATATGGCGAGA15320.10846417000838261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGCG250.00604058218.9952227
GGTATCA11200.014.0472131
GTGGTAT2850.012.7134381
GGACGGG751.4821848E-512.6634816
TAGGACC4100.012.27957254
AGGACGG550.003069920712.0900085
TGGTATC2850.011.9991052
TATACTG2000.011.399155
TCCAACG855.331639E-511.1744518
GCCGGTT600.00588622111.08054411
CGTCCAG600.00588622111.08054410
GTAGGAC14150.010.8755153
TCTATAC1408.354618E-910.8563343
TGTAGGA14300.010.8278642
GTGCTAG1502.0045263E-910.8064221
AGGACCT9050.010.6014195
CTGTAGG13650.010.5479621
GGACCTG8850.010.409816
TAGGACG15900.010.0967314
GACGTGG7750.010.0490857
Produced by FastQC (version 0.11.5)