##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511826_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1412448 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.428717375790118 31.0 30.0 33.0 26.0 34.0 2 29.64353873558531 31.0 30.0 34.0 26.0 34.0 3 29.706662475361924 31.0 30.0 34.0 26.0 34.0 4 33.31416731801808 35.0 33.0 37.0 28.0 37.0 5 32.802581758762095 35.0 33.0 37.0 26.0 37.0 6 32.73456297152178 35.0 33.0 37.0 26.0 37.0 7 32.49773938580394 35.0 33.0 37.0 25.0 37.0 8 32.34256694759736 35.0 32.0 37.0 24.0 37.0 9 33.61445660300414 37.0 33.0 39.0 25.0 39.0 10 33.321253596592584 37.0 33.0 39.0 23.0 39.0 11 33.351761622374774 37.0 33.0 39.0 22.0 39.0 12 33.07039197195224 37.0 32.0 39.0 19.0 39.0 13 33.05061850064569 37.0 33.0 39.0 19.0 39.0 14 33.734824220077485 38.0 33.0 40.0 18.0 41.0 15 33.649279831894695 38.0 33.0 40.0 17.0 41.0 16 33.493763310224516 38.0 32.0 40.0 17.0 41.0 17 33.40984022066653 38.0 32.0 40.0 17.0 41.0 18 33.28836672217314 38.0 32.0 40.0 17.0 41.0 19 33.3003197285847 38.0 32.0 40.0 16.0 41.0 20 33.11596391513174 38.0 32.0 40.0 11.0 41.0 21 32.94930999229706 38.0 32.0 40.0 10.0 41.0 22 33.01538109721562 38.0 32.0 40.0 10.0 41.0 23 32.852982198282696 38.0 32.0 40.0 2.0 41.0 24 32.63986851197354 38.0 32.0 40.0 2.0 41.0 25 32.08768535195632 37.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.635990372824363 1101 2 0.5945579481317402 1101 3 0.6908353620563474 1101 4 0.813775784629982 1101 5 0.7665631903533381 1101 6 0.7405770319119043 1101 7 0.731818269844851 1101 8 0.603915952069201 1101 9 0.7855164703242039 1101 10 0.8423229753320811 1101 11 0.6258331277230553 1101 12 0.8443765376823578 1101 13 0.7685074595160515 1101 14 0.5977834137630609 1101 15 0.5673083087744288 1101 16 0.8159726423909106 1101 17 0.6782546362823112 1101 18 0.8654129129580497 1101 19 0.9663266814807798 1101 20 0.9187410218448875 1101 21 1.003652621835137 1101 22 0.436847160245172 1101 23 0.5326049559918786 1101 24 0.609048704207531 1101 25 0.37461628276389547 1102 1 0.759885386035986 1102 2 0.6525456766275539 1102 3 0.7743907848555089 1102 4 0.7442390742179938 1102 5 0.8207924183326014 1102 6 0.6144971430052664 1102 7 0.8108961316339034 1102 8 1.0569725278873037 1102 9 0.9126754445107181 1102 10 0.8459781170844209 1102 11 0.9684278678993934 1102 12 1.0975352853697657 1102 13 1.2586698316837683 1102 14 1.40325480143607 1102 15 1.4310062875418623 1102 16 1.5714228390445584 1102 17 1.5755822929610943 1102 18 1.4644788266717796 1102 19 1.486005646191927 1102 20 1.3979938517237755 1102 21 1.5095245370829318 1102 22 1.4268480652525284 1102 23 1.6287810278939716 1102 24 1.586766730618912 1102 25 1.5623444818536427 1103 1 0.6377501526322789 1103 2 0.5880268518562666 1103 3 0.6392907965843406 1103 4 0.5779418656794064 1103 5 0.7825300444175198 1103 6 0.9717269889666369 1103 7 0.7775530242760169 1103 8 0.6537466110837968 1103 9 1.0140653108020672 1103 10 1.0370114269600919 1103 11 0.9692146768758576 1103 12 0.8917529724445927 1103 13 0.8102253676912738 1103 14 1.2173069165349801 1103 15 1.3001721722865653 1103 16 0.6012468088623706 1103 17 0.9769545660082457 1103 18 1.0013034415752173 1103 19 1.1476166986588865 1103 20 1.0957165040030787 1103 21 0.8031846160570524 1103 22 1.2967597080705744 1103 23 1.290478091776997 1103 24 1.3765020500340341 1103 25 0.9833141628294868 1104 1 0.32896263288504457 1104 2 0.4491177455597182 1104 3 0.5874021145712511 1104 4 0.4257696697868312 1104 5 0.6754238607118097 1104 6 0.558659020863125 1104 7 0.3438830475965631 1104 8 0.49191754691469214 1104 9 0.7850368747831098 1104 10 0.6153515307741344 1104 11 0.5653508011517943 1104 12 0.7679095962315472 1104 13 0.9011684392326842 1104 14 0.9880875905739543 1104 15 0.8696100343192157 1104 16 1.0770710208536443 1104 17 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-1.5350715898348035 2315 20 -1.2374534306374443 2315 21 -1.2945401521448048 2315 22 -1.4936795684152102 2315 23 -1.4236131454931034 2315 24 -1.2996911404204425 2315 25 -1.2490872279593894 2316 1 -1.8512029909027135 2316 2 -1.4277872050832379 2316 3 -1.2617694050763966 2316 4 -1.318825631354997 2316 5 -1.3455096946097633 2316 6 -1.502437700633994 2316 7 -1.8665256629521352 2316 8 -1.9746317005018064 2316 9 -2.0748136927566563 2316 10 -1.9977157379272121 2316 11 -1.8265198031557723 2316 12 -1.9772268614818564 2316 13 -1.7939931969587875 2316 14 -1.9003136844930921 2316 15 -1.9943760681246623 2316 16 -2.0684027561831044 2316 17 -1.977315896625207 2316 18 -1.97839209025555 2316 19 -2.0791008774581528 2316 20 -1.758722009243261 2316 21 -2.0621154037136087 2316 22 -1.8933154204387144 2316 23 -2.0004941304452224 2316 24 -1.8492887763795665 2316 25 -1.7671232630305376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46165.0 3 1.0 4 747.0 5 6351.0 6 7850.0 7 6304.0 8 5110.0 9 4729.0 10 4328.0 11 4033.0 12 3932.0 13 3690.0 14 3549.0 15 3726.0 16 3767.0 17 3989.0 18 4202.0 19 4860.0 20 5830.0 21 7161.0 22 9297.0 23 11562.0 24 13689.0 25 15900.0 26 18619.0 27 20895.0 28 24289.0 29 28856.0 30 36464.0 31 46471.0 32 58137.0 33 74627.0 34 96418.0 35 128228.0 36 180631.0 37 262311.0 38 255730.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.02065250988571 21.70002167216761 14.36459691544657 22.914728902500116 2 16.411294433494188 22.92225979292689 39.188062144588685 21.478383628990237 3 19.65467047282449 26.774720202088854 30.771681506151022 22.798927818935635 4 14.28045492648225 19.43137021681506 37.82553410815832 28.46264074854437 5 12.922586004338191 39.07467483034242 35.92987510170957 12.07286406360982 6 30.771964702417364 39.1048024422846 17.88313622873196 12.240096626566075 7 26.890476675955505 32.63426334987199 23.08835440313555 17.38690557103695 8 24.653296970932736 38.12196979995016 20.6628491809964 16.56188404812071 9 26.54957917034822 15.136911234962277 20.686496069235822 37.62701352545368 10 14.928903577335237 30.108081855048823 34.86209757810553 20.10091698951041 11 34.136548743741365 22.63049683952967 25.21190160628922 18.02105281043975 12 23.133595006683432 25.581189537597137 32.31998629330071 18.965229162418723 13 29.30550363624006 22.55693660934774 26.005842338974606 22.131717415437596 14 20.637503115158932 23.11731122136886 28.333857246426064 27.91132841704615 15 22.82314109970774 32.137891094043816 23.694536011237226 21.344431795011214 16 21.72164922177666 28.789803235234146 28.43460431817667 21.053943224812524 17 21.39321235188835 29.419560932508666 28.29463456353791 20.892592152065067 18 21.122663183345193 27.8560227123888 30.68827458768306 20.333039516582947 19 23.33799191191463 27.291482589093548 28.455065248419764 20.915460250572057 20 23.372966650807676 27.793943564648043 28.88998391445207 19.94310587009221 21 22.812946034119484 27.106484628106664 28.10963660255103 21.97093273522282 22 22.896559731756497 28.569830535354225 28.26341217517388 20.2701975577154 23 22.054475633793245 28.377965064908583 28.636310858877636 20.931248442420536 24 22.94134139917416 27.305335123224978 29.182455663364433 20.570867814236436 25 22.59567969630011 27.88586049600445 28.87605694231359 20.64240286538185 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 347.0 1 347.0 2 481.0 3 615.0 4 615.0 5 615.0 6 1431.0 7 2247.0 8 2247.0 9 2247.0 10 3249.5 11 4252.0 12 4252.0 13 4252.0 14 6309.0 15 8366.0 16 8366.0 17 8366.0 18 14795.5 19 21225.0 20 21225.0 21 21225.0 22 32828.5 23 44432.0 24 44432.0 25 44432.0 26 63056.5 27 81681.0 28 81681.0 29 81681.0 30 102616.5 31 123552.0 32 123552.0 33 123552.0 34 136541.0 35 149530.0 36 149530.0 37 149530.0 38 156446.0 39 163362.0 40 163362.0 41 163362.0 42 168735.5 43 174109.0 44 174109.0 45 174109.0 46 174559.0 47 175009.0 48 175009.0 49 175009.0 50 167178.0 51 159347.0 52 159347.0 53 159347.0 54 140030.0 55 120713.0 56 120713.0 57 120713.0 58 102508.5 59 84304.0 60 84304.0 61 84304.0 62 67960.5 63 51617.0 64 51617.0 65 51617.0 66 39587.0 67 27557.0 68 27557.0 69 27557.0 70 20164.5 71 12772.0 72 12772.0 73 12772.0 74 8831.5 75 4891.0 76 4891.0 77 4891.0 78 3340.5 79 1790.0 80 1790.0 81 1790.0 82 1156.0 83 522.0 84 522.0 85 522.0 86 341.5 87 161.0 88 161.0 89 161.0 90 100.5 91 40.0 92 40.0 93 40.0 94 23.5 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3619956274496477 2 0.0 3 0.0 4 0.0 5 0.02400088357235098 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 2.1239719975531843E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005097532794127642 25 7.079906658510613E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1412448.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.44528657007183 #Duplication Level Percentage of deduplicated Percentage of total 1 85.82018547182966 63.88908300947074 2 10.081628612932905 15.010594623656514 3 2.3253982957222896 5.193448275438073 4 0.7735909990820493 2.303608144587653 5 0.3405931872313552 1.2677778713626182 6 0.1748606397718626 0.7810530262585446 7 0.10038450588370149 0.5231207315395053 8 0.06686314604525949 0.39821168546527264 9 0.04926304899764341 0.33006616199505473 >10 0.22497497973331193 3.0338397704217437 >50 0.021042354928493073 1.0928193792869045 >100 0.017237086411064306 2.506406711872939 >500 0.0025570744910113495 1.2399625976260398 >1k 0.0013258904768207 2.040259148808912 >5k 9.470646263004999E-5 0.38974886220940175 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5528 0.3913772400824667 No Hit TATCAACGCAGAGTACTTTTTTTTT 4100 0.29027617299893516 No Hit GGTATCAACGCAGAGTACTTTTTTT 4023 0.28482464487188197 No Hit GTCCTACAGTGGACATTTCTAAATT 2557 0.18103321325811642 No Hit CTGTAGGACGTGGAATATGGCAAGA 2542 0.1799712272593398 No Hit CTTTAGGACGTGAAATATGGCGAGG 2539 0.1797588300595845 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2489 0.17621887673032918 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1954 0.1383413761072974 No Hit CTGAAGGACCTGGAATATGGCGAGA 1532 0.10846417000838261 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 7.079906658510615E-5 0.0 11 0.0 0.0 0.0 7.079906658510615E-5 0.0 12 0.0 0.0 0.0 7.079906658510615E-5 7.079906658510615E-5 13 0.0 0.0 0.0 7.079906658510615E-5 7.079906658510615E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGCG 25 0.006040582 18.995222 7 GGTATCA 1120 0.0 14.047213 1 GTGGTAT 285 0.0 12.713438 1 GGACGGG 75 1.4821848E-5 12.663481 6 TAGGACC 410 0.0 12.2795725 4 AGGACGG 55 0.0030699207 12.090008 5 TGGTATC 285 0.0 11.999105 2 TATACTG 200 0.0 11.39915 5 TCCAACG 85 5.331639E-5 11.17445 18 GCCGGTT 60 0.005886221 11.080544 11 CGTCCAG 60 0.005886221 11.080544 10 GTAGGAC 1415 0.0 10.875515 3 TCTATAC 140 8.354618E-9 10.856334 3 TGTAGGA 1430 0.0 10.827864 2 GTGCTAG 150 2.0045263E-9 10.806422 1 AGGACCT 905 0.0 10.601419 5 CTGTAGG 1365 0.0 10.547962 1 GGACCTG 885 0.0 10.40981 6 TAGGACG 1590 0.0 10.096731 4 GACGTGG 775 0.0 10.049085 7 >>END_MODULE