FastQCFastQC Report
Thu 26 May 2016
SRR1511825_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511825_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048200
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64840.31657064739771507No Hit
GGTATCAACGCAGAGTACTTTTTTT43780.2137486573576799No Hit
TATCAACGCAGAGTACTTTTTTTTT41360.20193340494092374No Hit
GTCCTACAGTGGACATTTCTAAATT30380.14832535885167464No Hit
GTCCTAAAGTGTGTATTTCTCATTT27930.13636363636363638No Hit
CTGTAGGACGTGGAATATGGCAAGA27550.13450834879406307No Hit
CTTTAGGACGTGAAATATGGCGAGG25020.12215603944927254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGCG400.00548631714.167837
CTACGCT552.0587545E-413.7388374
CTAGTAC707.710367E-613.4935013
GGTATCA12100.012.5507591
CGAACCG1001.5561454E-712.27878712
AACCGGG1104.1358362E-812.0211914
GTCAACG751.297824E-411.9892782
AAGTCGT1603.6379788E-1211.8065267
TAGGACC6000.011.6493894
CAAGTCG1501.9463187E-1011.334546
AATCCCG1355.175025E-911.19488119
CACGACT600.006134033711.0196924
TATCGTG600.006134033711.0196925
TCGTCAA1553.45608E-1010.96864310
GTATCAA25800.010.8042921
AGTCGTC1752.3646862E-1110.7945388
GGCGAGG8350.010.74658419
GCGAACC1159.501491E-710.67720511
TAAGGGT1201.640934E-610.2325724
GGCGAAC1201.6414469E-610.23232210
Produced by FastQC (version 0.11.5)