FastQCFastQC Report
Thu 26 May 2016
SRR1511825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048200
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86200.42085733815057125No Hit
GGTATCAACGCAGAGTACTTTTTTT56330.2750219705106923No Hit
TATCAACGCAGAGTACTTTTTTTTT54060.26393906845034665No Hit
GTCCTACAGTGGACATTTCTAAATT34520.16853822868860463No Hit
CTGTAGGACGTGGAATATGGCAAGA32190.15716238648569475No Hit
GTCCTAAAGTGTGTATTTCTCATTT30680.14979005956449565No Hit
CTTTAGGACGTGAAATATGGCGAGG27700.1352406991504736No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26600.12987012987012986No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21030.10267551996875304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.013.5671621
GTCGGTA604.1014064E-412.66359115
GCGCCAC1001.447861E-712.34713
AAGGCGT1650.012.0876786
TCCAACG1353.7289283E-1011.96093418
TATCGCG802.8752567E-511.8718267
TGGTATC4700.011.5201652
AAGTCGT1158.85022E-710.736267
GGCGAGG7550.010.69328919
GTGGTAT5450.010.6736991
GTGCGCC1252.249144E-710.637156511
AGCGTAC1002.3003853E-510.4899711
TAGGACC5350.010.4756264
GGCGTGC1856.002665E-1110.2675268
CCAACGA750.002652451910.13136719
GTATCAA30400.010.069621
CGCGCCT856.6095917E-410.0563812
CGACTAA951.648437E-49.99757117
GTCTAGT1451.5298792E-79.8651771
GCCTCGA1451.623157E-79.82519916
Produced by FastQC (version 0.11.5)