FastQCFastQC Report
Thu 26 May 2016
SRR1511824_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511824_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321824
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63980.2755592155133206No Hit
GTCCTACAGTGGACATTTCTAAATT43760.18847251126700387No Hit
CTGTAGGACGTGGAATATGGCAAGA42570.1833472304532988No Hit
GGTATCAACGCAGAGTACTTTTTTT40780.17563777443940626No Hit
TATCAACGCAGAGTACTTTTTTTTT39210.1688758493322491No Hit
GTCCTAAAGTGTGTATTTCTCATTT38730.1668085091720992No Hit
CTTTAGGACGTGAAATATGGCGAGG35870.15449060738453904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAA350.002256442316.19144610
CCGTTCA509.15927E-515.1120189
TCGCCCG400.005486635514.167825
GGTATCA13500.013.6205771
CGAACCG803.0432668E-511.80626212
TAGGACC6400.011.6589364
CTAGTAC1002.0698026E-611.3342563
GTGGTAT4750.011.1504831
GACCGTT855.6495326E-511.1117767
AGACCCG1451.3405952E-911.0736995
ATCGCGC600.006136582211.0191788
GGACCGT1159.506275E-710.6769686
GTCCTAC23250.010.6595821
GTATTAG1602.6848284E-910.6179431
GTATCAA28400.010.1692971
GTCCTAG3650.010.1302541
TGGTATC4750.010.0985512
CTAGCAC2250.010.0748943
TCCTACA26100.010.0699352
CCTACAG27050.09.8118483
Produced by FastQC (version 0.11.5)