FastQCFastQC Report
Thu 26 May 2016
SRR1511824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321824
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85230.36708208718662566No Hit
GGTATCAACGCAGAGTACTTTTTTT57260.24661645327122123No Hit
TATCAACGCAGAGTACTTTTTTTTT54950.23666737875049962No Hit
GTCCTACAGTGGACATTTCTAAATT50290.21659695136237717No Hit
CTGTAGGACGTGGAATATGGCAAGA47720.20552806758824096No Hit
GTCCTAAAGTGTGTATTTCTCATTT43470.18722349325357995No Hit
CTTTAGGACGTGAAATATGGCGAGG41060.1768437228661604No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25680.11060269856802238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.013.2109261
GGCGAGG11750.011.47804119
GACGTGA13800.011.1489647
TGCGATA600.005889748711.08014210
TTAGGAC16050.011.0092583
TAGGACC7300.010.931424
GGACGTG26450.010.9155716
AGGACGT26750.010.9027185
GTGGTAT5300.010.6177011
CCTATAC1803.45608E-1110.5554713
TATGTCG1355.7150828E-810.55251616
ACGTGAA16300.010.5460428
GTAATAC1909.094947E-1210.4999163
CGTGAAA16550.010.3867379
TGGTATC4950.010.3635542
ACCTTTT6350.010.17029915
TGTCGAG1502.4554538E-810.13085218
TAGGACG28800.010.1266564
ATGTCGA1606.204573E-910.09084417
TGTAGGA25300.09.7627692
Produced by FastQC (version 0.11.5)