FastQCFastQC Report
Thu 26 May 2016
SRR1511823_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511823_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778053
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71440.4017877982264871No Hit
GGTATCAACGCAGAGTACTTTTTTT43680.2456619684565083No Hit
GTCCTACAGTGGACATTTCTAAATT41090.23109547353200385No Hit
TATCAACGCAGAGTACTTTTTTTTT41090.23109547353200385No Hit
CTGTAGGACGTGGAATATGGCAAGA38220.21495422239944478No Hit
GTCCTAAAGTGTGTATTTCTCATTT30270.17024239434932478No Hit
CTTTAGGACGTGAAATATGGCGAGG29670.1668679167606365No Hit
GTCCTACAGTGTGCATTTCTCATTT20640.11608202905087756No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18940.1065210092162607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGA350.001543352417.1374472
CCAACGT801.3830868E-714.16786719
GGTATCA12350.014.0846231
TGTCGAG1350.013.99295418
TCCAACG1102.0372681E-1013.73853718
CGTTAGG450.008216538513.3291261
TAGGACC6850.012.8227494
ATGTCGA1700.012.7788617
CGGTAGG655.22807E-412.3038091
CCTGCGA550.003206502912.02020558
GTCGAGG951.1152206E-611.93083519
GGCGAGG10700.011.122437519
TATGTCG1803.6379788E-1211.01945216
TACACCG600.006137063311.0188315
TGGCGAA700.001565252210.79456418
GTATCAA26050.010.5149151
GATATAC4300.010.4618141
TGTAGGA23450.010.4444652
AATCCCG1901.0913936E-1110.43948119
AGGACGT24450.010.4297455
Produced by FastQC (version 0.11.5)