##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511823_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1778053 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.319731751528217 31.0 30.0 34.0 25.0 34.0 2 26.571471716534884 31.0 26.0 34.0 10.0 34.0 3 28.329300082730942 31.0 28.0 34.0 16.0 34.0 4 32.68864313943398 35.0 32.0 37.0 26.0 37.0 5 33.06595078999332 35.0 35.0 37.0 27.0 37.0 6 33.27345022898643 37.0 35.0 37.0 27.0 37.0 7 33.26235269702309 37.0 35.0 37.0 27.0 37.0 8 33.27620211546 37.0 35.0 37.0 27.0 37.0 9 34.799201711085104 39.0 35.0 39.0 27.0 39.0 10 34.68590306363196 39.0 35.0 39.0 27.0 39.0 11 34.6692950097663 39.0 35.0 39.0 27.0 39.0 12 34.562775687788836 39.0 35.0 39.0 27.0 39.0 13 34.54639259909575 39.0 35.0 39.0 27.0 39.0 14 35.616950675823496 40.0 36.0 41.0 27.0 41.0 15 35.55711893852433 40.0 36.0 41.0 27.0 41.0 16 35.509255910819306 40.0 36.0 41.0 27.0 41.0 17 35.486679530925116 40.0 36.0 41.0 27.0 41.0 18 35.455646710193676 40.0 36.0 41.0 26.0 41.0 19 35.494485822413615 40.0 36.0 41.0 26.0 41.0 20 35.43586664739465 40.0 36.0 41.0 26.0 41.0 21 35.320045577943965 40.0 36.0 41.0 25.0 41.0 22 35.22758489201391 40.0 36.0 41.0 25.0 41.0 23 35.113947671976035 40.0 36.0 41.0 25.0 41.0 24 34.99989764084648 40.0 35.0 41.0 24.0 41.0 25 34.89600141278128 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1375651374953932 1101 2 3.783076772954434 1101 3 2.2907506198475502 1101 4 1.3957752069843892 1101 5 0.7883639543667869 1101 6 0.7947184305272543 1101 7 0.6143455071958996 1101 8 0.6594572514354411 1101 9 0.7903957732972415 1101 10 0.7484967210058997 1101 11 0.6549633976047744 1101 12 0.6847002292966025 1101 13 0.6388466005544942 1101 14 0.7068061197883111 1101 15 0.8191731971727378 1101 16 0.8278270122176252 1101 17 0.810461385777117 1101 18 1.0466930351260544 1101 19 1.092065827652462 1101 20 0.9575991394586865 1101 21 0.9707848718821879 1101 22 0.9157480186911471 1101 23 0.8999666009815499 1101 24 0.8820461571769016 1101 25 0.9321374935205 1102 1 1.2312800805646162 1102 2 3.840607505497342 1102 3 2.1525247104665652 1102 4 1.4453625008412345 1102 5 0.8520680654741639 1102 6 0.8227329067305291 1102 7 0.7911491547960736 1102 8 0.5575028800251047 1102 9 0.672379869626127 1102 10 0.8161586966213861 1102 11 0.6889862538839537 1102 12 0.7739597933311302 1102 13 1.0072785691166075 1102 14 1.1022130320914059 1102 15 1.020776579431832 1102 16 1.0552394328672108 1102 17 1.1570190890653294 1102 18 1.0372826633126664 1102 19 1.0231556573639438 1102 20 1.2172358069090663 1102 21 1.026978822777295 1102 22 1.0543757182847386 1102 23 1.2012458640722699 1102 24 0.7434927698796798 1102 25 0.6784974505285248 1103 1 0.9278520817816336 1103 2 3.3748625111701216 1103 3 1.9853400533294092 1103 4 1.191973153314244 1103 5 0.7138761155782163 1103 6 0.5058636950252264 1103 7 0.6239831834999663 1103 8 0.5048343997486171 1103 9 0.3920930663388873 1103 10 0.6427142827332588 1103 11 0.7310635182118332 1103 12 0.5646568442585789 1103 13 0.6243142879142027 1103 14 0.7855977040959985 1103 15 0.6803205555431973 1103 16 0.6499232298463582 1103 17 0.7457628196610031 1103 18 0.8109975344392666 1103 19 0.8426232165097076 1103 20 0.957468900589717 1103 21 0.9162709015649213 1103 22 0.88974086602272 1103 23 0.9499460810311362 1103 24 1.084969203175092 1103 25 0.8631862723740582 1104 1 1.2529482644303762 1104 2 3.843893336421928 1104 3 2.2505545414161787 1104 4 1.5418923380473402 1104 5 1.1948268026639965 1104 6 0.8551931467295262 1104 7 0.7555864772681389 1104 8 0.9087219573177947 1104 9 1.154076113854515 1104 10 1.1744190636271554 1104 11 1.0659347598338798 1104 12 1.1633637999880406 1104 13 1.1268561464781257 1104 14 1.39860178542299 1104 15 1.262555550113909 1104 16 1.2900948140752106 1104 17 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-1.531407181168106 2315 19 -1.541919392246605 2315 20 -1.419686969649696 2315 21 -1.5900869137462692 2315 22 -1.578793557852947 2315 23 -1.5764242906310386 2315 24 -1.9229165921622027 2315 25 -1.9966116052538325 2316 1 -2.1230260827075824 2316 2 -1.9047300479093856 2316 3 -1.4119399631120935 2316 4 -1.7546938749131833 2316 5 -1.9535377449487719 2316 6 -2.056125326848246 2316 7 -2.1130618565048316 2316 8 -2.420643645425546 2316 9 -2.474836112470989 2316 10 -2.484403916237433 2316 11 -2.6645403786520063 2316 12 -2.505782423523783 2316 13 -2.643422693757522 2316 14 -2.727380804922568 2316 15 -3.1937752333205367 2316 16 -3.009380917921625 2316 17 -3.0542394638806627 2316 18 -2.934183764496318 2316 19 -2.889256452262572 2316 20 -3.0396247217638717 2316 21 -2.9786693410042915 2316 22 -2.877487171301766 2316 23 -2.8471923558275094 2316 24 -2.78468738070368 2316 25 -2.886985706857125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70375.0 3 3.0 4 633.0 5 3245.0 6 3775.0 7 3381.0 8 3176.0 9 3045.0 10 2920.0 11 2820.0 12 2802.0 13 2672.0 14 2751.0 15 2848.0 16 3052.0 17 3232.0 18 3722.0 19 4544.0 20 5925.0 21 7618.0 22 9427.0 23 11455.0 24 13064.0 25 14829.0 26 16769.0 27 18983.0 28 21686.0 29 26101.0 30 32207.0 31 40471.0 32 51321.0 33 67735.0 34 91709.0 35 129781.0 36 199748.0 37 268575.0 38 631653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.30315575519965 20.33938245935301 14.166225641192923 24.191236144254418 2 14.998776681586588 21.69904938337013 41.85034797604796 21.451825958995318 3 18.2387139191014 25.749682377296967 30.48660529241817 25.524998411183468 4 11.465350020499951 18.015829674368536 40.72848222184603 29.790338083285484 5 12.136601870708413 38.73280904904999 36.58797903087833 12.542610049363262 6 29.05842494159306 39.20728531721973 19.282446646367184 12.451843094820033 7 26.277281948288376 32.63147948908159 23.277540095823916 17.81369846680611 8 23.35953990122904 38.64187400488062 21.46938252121843 16.529203572671904 9 25.37483415848684 16.28669111663151 21.854016725035756 36.4844579998459 10 14.189678260434308 30.12727967051601 35.498041959379165 20.185000109670522 11 32.26506746424319 23.54834192231615 26.214235458673052 17.9723551547676 12 22.68734396556233 25.63163190298602 33.01448269539772 18.666541436053933 13 27.901899326847207 23.429192975904517 26.704408366239214 21.964499331009065 14 19.57669428301631 24.128808308863682 29.11426149839178 27.180235909728225 15 21.404198862463605 33.00075981987038 24.54195684830542 21.053084469360588 16 20.058288476215274 29.884148560251017 29.90552025164604 20.152042711887667 17 19.941441335038576 30.255403965926643 29.39406517041741 20.40908952861737 18 19.506786355637317 28.709436670335474 32.32468323497669 19.459093739050523 19 21.866519838901898 27.984524593556863 29.56884716770704 20.5801083998342 20 21.95190675606818 28.54864272352909 30.384815690932633 19.114634829470102 21 21.243897775078178 27.978898112528398 29.132246743605318 21.644957368788102 22 21.277801993841308 29.745918812131777 29.496871440824535 19.47940775320238 23 20.346997920757055 29.435769840122695 29.878558039111365 20.338674200008885 24 21.365905237019938 27.998245234151568 30.90750078036462 19.72834874846388 25 20.92179235965976 28.82078878042964 30.273009095428886 19.984409764481708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 170.0 2 387.5 3 605.0 4 605.0 5 605.0 6 1596.0 7 2587.0 8 2587.0 9 2587.0 10 4075.5 11 5564.0 12 5564.0 13 5564.0 14 8977.5 15 12391.0 16 12391.0 17 12391.0 18 21140.5 19 29890.0 20 29890.0 21 29890.0 22 46103.5 23 62317.0 24 62317.0 25 62317.0 26 88002.5 27 113688.0 28 113688.0 29 113688.0 30 144076.5 31 174465.0 32 174465.0 33 174465.0 34 192245.5 35 210026.0 36 210026.0 37 210026.0 38 218338.0 39 226650.0 40 226650.0 41 226650.0 42 230378.5 43 234107.0 44 234107.0 45 234107.0 46 229949.5 47 225792.0 48 225792.0 49 225792.0 50 207852.0 51 189912.0 52 189912.0 53 189912.0 54 159546.5 55 129181.0 56 129181.0 57 129181.0 58 105250.0 59 81319.0 60 81319.0 61 81319.0 62 63122.5 63 44926.0 64 44926.0 65 44926.0 66 33080.5 67 21235.0 68 21235.0 69 21235.0 70 14977.0 71 8719.0 72 8719.0 73 8719.0 74 5916.0 75 3113.0 76 3113.0 77 3113.0 78 2039.5 79 966.0 80 966.0 81 966.0 82 628.5 83 291.0 84 291.0 85 291.0 86 197.0 87 103.0 88 103.0 89 103.0 90 62.5 91 22.0 92 22.0 93 22.0 94 15.5 95 9.0 96 9.0 97 9.0 98 7.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.522670021647274 3 0.0 4 0.0 5 5.624129314480502E-4 6 6.186542245928552E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.1248258628961003E-4 14 0.0 15 0.0 16 0.0 17 3.936890520136351E-4 18 0.0 19 3.3744775886883013E-4 20 3.936890520136351E-4 21 7.311368108824653E-4 22 0.004386820865294791 23 1.1248258628961003E-4 24 0.002137169139502591 25 9.561019834616852E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1778053.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.86555509835009 #Duplication Level Percentage of deduplicated Percentage of total 1 89.46271050010628 70.55526324193875 2 8.075672258764257 12.737847509595799 3 1.3662944223865598 3.232607041478869 4 0.41513053203539746 1.3095799938898005 5 0.18600707146642403 0.7334775471709002 6 0.10180098526153172 0.4817154727325792 7 0.07145544601345168 0.3944761390255737 8 0.04723041169549586 0.297988210871111 9 0.035534766613303424 0.2522222184823643 >10 0.20012039833102604 2.845829969454555 >50 0.019583066876722323 1.048458881588855 >100 0.01507163749352363 2.3564342207925395 >500 0.0020186826121685536 1.1536642170379516 >1k 0.0012977245363940703 2.194236362726052 >5k 7.209580757744835E-5 0.4061989732143482 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7144 0.4017877982264871 No Hit GGTATCAACGCAGAGTACTTTTTTT 4368 0.2456619684565083 No Hit GTCCTACAGTGGACATTTCTAAATT 4109 0.23109547353200385 No Hit TATCAACGCAGAGTACTTTTTTTTT 4109 0.23109547353200385 No Hit CTGTAGGACGTGGAATATGGCAAGA 3822 0.21495422239944478 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3027 0.17024239434932478 No Hit CTTTAGGACGTGAAATATGGCGAGG 2967 0.1668679167606365 No Hit GTCCTACAGTGTGCATTTCTCATTT 2064 0.11608202905087756 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1894 0.1065210092162607 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.624129314480502E-5 0.0 5 0.0 0.0 0.0 1.1248258628961004E-4 0.0 6 0.0 0.0 0.0 1.1248258628961004E-4 0.0 7 0.0 0.0 0.0 1.1248258628961004E-4 0.0 8 0.0 0.0 0.0 1.1248258628961004E-4 0.0 9 0.0 0.0 0.0 1.1248258628961004E-4 0.0 10 0.0 0.0 0.0 1.1248258628961004E-4 0.0 11 0.0 0.0 0.0 1.1248258628961004E-4 0.0 12 0.0 0.0 0.0 1.1248258628961004E-4 0.0 13 0.0 0.0 0.0 1.1248258628961004E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAGGA 35 0.0015433524 17.137447 2 CCAACGT 80 1.3830868E-7 14.167867 19 GGTATCA 1235 0.0 14.084623 1 TGTCGAG 135 0.0 13.992954 18 TCCAACG 110 2.0372681E-10 13.738537 18 CGTTAGG 45 0.0082165385 13.329126 1 TAGGACC 685 0.0 12.822749 4 ATGTCGA 170 0.0 12.77886 17 CGGTAGG 65 5.22807E-4 12.303809 1 CCTGCGA 55 0.0032065029 12.0202055 8 GTCGAGG 95 1.1152206E-6 11.930835 19 GGCGAGG 1070 0.0 11.1224375 19 TATGTCG 180 3.6379788E-12 11.019452 16 TACACCG 60 0.0061370633 11.018831 5 TGGCGAA 70 0.0015652522 10.794564 18 GTATCAA 2605 0.0 10.514915 1 GATATAC 430 0.0 10.461814 1 TGTAGGA 2345 0.0 10.444465 2 AATCCCG 190 1.0913936E-11 10.439481 19 AGGACGT 2445 0.0 10.429745 5 >>END_MODULE