FastQCFastQC Report
Thu 26 May 2016
SRR1511823_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511823_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1778053
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93830.5277120535777056No Hit
GGTATCAACGCAGAGTACTTTTTTT59440.33429824645272105No Hit
TATCAACGCAGAGTACTTTTTTTTT53550.3011721247904309No Hit
GTCCTACAGTGGACATTTCTAAATT45900.2581475355346551No Hit
CTGTAGGACGTGGAATATGGCAAGA43110.24245621474725446No Hit
CTTTAGGACGTGAAATATGGCGAGG33770.18992684695000656No Hit
GTCCTAAAGTGTGTATTTCTCATTT33360.18762095393106956No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26220.14746467062567878No Hit
GTCCTACAGTGTGCATTTCTCATTT23150.13019859363022362No Hit
CTGTAGGACCTGGAATATGGCGAGA17960.10100936248806981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.013.3189291
AGAACCG500.001500264613.2993315
GCCGGTT1403.6379788E-1212.88894611
CGGTTTC1457.2759576E-1212.44484813
CCAACGA1155.342372E-912.3885919
TCCAACG2150.012.36938218
CCAACGT1001.4469879E-712.34729419
GGCGAGG9500.012.0973519
TACACGG550.003069901612.0903015
TAGGACC7950.012.0685754
CCGGTTT1604.5474735E-1111.27814512
TGCCGGT1604.5474735E-1111.27782710
GTATCAA29850.011.0572011
ACTATCG951.364972E-510.99666211
TAGACTG2350.010.9143445
TGTAGGA24050.010.9017382
CTGTAGG23650.010.5677761
TTAGGAC14700.010.4688613
TAGGACA6950.010.2512834
CCCGTTT1054.1175488E-59.94964112
Produced by FastQC (version 0.11.5)