FastQCFastQC Report
Thu 26 May 2016
SRR1511822_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511822_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140202
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66870.31244714283978803No Hit
GTCCTACAGTGGACATTTCTAAATT48220.22530583561738565No Hit
CTGTAGGACGTGGAATATGGCAAGA45020.21035397593311286No Hit
GTCCTAAAGTGTGTATTTCTCATTT42930.20058854257682218No Hit
GGTATCAACGCAGAGTACTTTTTTT42310.19769161976299432No Hit
TATCAACGCAGAGTACTTTTTTTTT40870.19096328290507159No Hit
CTTTAGGACGTGAAATATGGCGAGG40530.1893746478136176No Hit
GTCCTACAGTGTGCATTTCTCATTT26170.12227817748044344No Hit
CTGAAGGACCTGGAATATGGCGAGA21870.10218661602970187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.014.7004661
GCGTCAG400.00548514414.16832813
TCGTAAT400.00548599414.16799714
AAAATCG400.00548599414.16799715
CGGTAGG1202.1827873E-1114.1522451
TAGGACC8750.013.3856984
TAGAACG854.2070315E-612.2236574
GGCGAGG13550.011.64138219
GACCGTC752.1882304E-411.3346647
TGTAGGA26700.010.9626112
GTAGGAC28700.010.8936523
ACTGCGC700.001565051510.7949178
GCGCCAC700.001565051510.79491713
GTATCAA25900.010.7226641
GACGTGG13450.010.6745287
AGGACCT17550.010.6565215
AGGACGT29950.010.470535
GGACGTG29150.010.4336096
CTGTAGG27050.010.3406641
GGACCTG16950.010.3090656
Produced by FastQC (version 0.11.5)