##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511822_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2140202 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.250873982923107 31.0 30.0 34.0 24.0 34.0 2 26.527173135993706 31.0 26.0 34.0 10.0 34.0 3 28.264436721393587 31.0 28.0 34.0 16.0 34.0 4 32.61346265445972 35.0 32.0 37.0 26.0 37.0 5 32.98485376613983 35.0 35.0 37.0 26.0 37.0 6 33.181828631129214 37.0 35.0 37.0 27.0 37.0 7 33.16380463152544 37.0 35.0 37.0 26.0 37.0 8 33.1791358946492 37.0 35.0 37.0 26.0 37.0 9 34.70802989624344 39.0 35.0 39.0 27.0 39.0 10 34.56994807032233 39.0 35.0 39.0 27.0 39.0 11 34.57181238032672 39.0 35.0 39.0 27.0 39.0 12 34.45216806637878 39.0 35.0 39.0 27.0 39.0 13 34.4380619212579 39.0 35.0 39.0 27.0 39.0 14 35.50211989335586 40.0 36.0 41.0 26.0 41.0 15 35.44457766136094 40.0 36.0 41.0 26.0 41.0 16 35.39038417868967 40.0 36.0 41.0 26.0 41.0 17 35.35437122290326 40.0 36.0 41.0 26.0 41.0 18 35.32157058072089 40.0 36.0 41.0 25.0 41.0 19 35.34686118413122 40.0 36.0 41.0 25.0 41.0 20 35.285215133898575 40.0 36.0 41.0 25.0 41.0 21 35.17590629295739 40.0 36.0 41.0 25.0 41.0 22 35.06946493835628 40.0 35.0 41.0 24.0 41.0 23 34.95334599257453 39.0 35.0 41.0 24.0 41.0 24 34.83916564884997 39.0 35.0 41.0 22.0 41.0 25 34.7292797595741 39.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9365294306802454 1101 2 3.5065083827707397 1101 3 2.0747491100324247 1101 4 1.1467771856410636 1101 5 0.6346504405947755 1101 6 0.7206839945404155 1101 7 0.5369784219117264 1101 8 0.5304750496123134 1101 9 0.6594464422503279 1101 10 0.6496110941724922 1101 11 0.453845605200911 1101 12 0.5280288688320596 1101 13 0.4309694566169995 1101 14 0.6089482785497893 1101 15 0.714808729894763 1101 16 0.7410262152761291 1101 17 0.7408369452427266 1101 18 0.8336172475555159 1101 19 0.8026364781179751 1101 20 0.7085272294277871 1101 21 0.7428698021317075 1101 22 0.8075253181236874 1101 23 0.6530150799656198 1101 24 0.7239144959336699 1101 25 0.7966816314843115 1102 1 1.6707892971259852 1102 2 4.298398729261461 1102 3 2.6862115429816207 1102 4 1.8376765565565023 1102 5 1.4621161087752554 1102 6 1.390134015850002 1102 7 1.2521388390057382 1102 8 1.3248609030214737 1102 9 1.452864064482867 1102 10 1.4813817243517562 1102 11 1.4224732145626788 1102 12 1.4725878028637425 1102 13 1.4891265651449785 1102 14 1.5636274071629828 1102 15 1.5492991225119752 1102 16 1.6684891201184513 1102 17 1.7187392739928171 1102 18 1.5982977472095712 1102 19 1.847577921919651 1102 20 1.8533203032269867 1102 21 1.7408589422127605 1102 22 1.902397093919184 1102 23 2.1215688231728507 1102 24 1.7687671940676708 1102 25 1.7937859719445726 1103 1 1.1932872979549636 1103 2 3.8803495643078705 1103 3 2.3075826812321445 1103 4 1.5640350508353151 1103 5 0.9874862170863636 1103 6 0.7964477328846158 1103 7 0.8068161665222817 1103 8 0.6102546036440444 1103 9 0.7886599020203917 1103 10 0.9435832617247399 1103 11 1.0913382715652205 1103 12 0.9764385621300562 1103 13 1.1021791632299625 1103 14 1.3109353297416888 1103 15 1.1028345151659096 1103 16 1.0417432472029304 1103 17 1.164593139824369 1103 18 1.1852213893433046 1103 19 1.1936830971558905 1103 20 1.0771636100861315 1103 21 1.2700573060325837 1103 22 1.2013992877738744 1103 23 1.4204961789499322 1103 24 1.4038928383031504 1103 25 1.3571576000443386 1104 1 1.3575643710982916 1104 2 3.8805938234653325 1104 3 2.317360570913273 1104 4 1.6481665314771519 1104 5 1.3913840314935442 1104 6 1.1546294616977661 1104 7 0.9657586597913621 1104 8 1.0225199242605214 1104 9 1.088674346915127 1104 10 1.0817883029930044 1104 11 1.0514894213387151 1104 12 1.0926166436072862 1104 13 1.038077936617654 1104 14 1.1544900979613146 1104 15 1.0921989598600206 1104 16 1.2255259438240316 1104 17 1.1320269240256664 1104 18 1.1421640671860374 1104 19 1.1903963820453214 1104 20 1.1534336573931583 1104 21 1.1607756586725841 1104 22 1.1103538184552306 1104 23 1.3853750223953654 1104 24 1.3718477421442898 1104 25 1.3534711425343957 1105 1 1.1556417337159886 1105 2 3.810095822646403 1105 3 2.196588831581977 1105 4 1.4420690048984 1105 5 1.0680282861943695 1105 6 0.75757050944177 1105 7 0.5865751387622709 1105 8 0.680806824007739 1105 9 0.7806759692133625 1105 10 0.8660289594596762 1105 11 0.9164734560955807 1105 12 0.7596496565983202 1105 13 0.8917243631361984 1105 14 0.8793053281127143 1105 15 1.0134999885799516 1105 16 0.8574140246977322 1105 17 0.8927748929919161 1105 18 1.0405982770596864 1105 19 1.1017720189773144 1105 20 1.0559780785059871 1105 21 1.165906614515535 1105 22 1.1117310212746077 1105 23 1.0815058216495714 1105 24 1.1560149672459517 1105 25 1.0922008869057152 1106 1 0.8983756597164039 1106 2 3.6303562196586654 1106 3 2.0936240679303886 1106 4 0.967528204875272 1106 5 0.8183404766173581 1106 6 0.6149166507991168 1106 7 0.5286674934394995 1106 8 0.4247587959826191 1106 9 0.397221984197472 1106 10 0.6568002027338409 1106 11 0.44594015380491214 1106 12 0.46801501773514076 1106 13 0.43835211290273435 1106 14 0.48154711088393753 1106 15 0.43384690583180685 1106 16 0.6054000802782156 1106 17 0.3597147144484225 1106 18 0.4553138933950791 1106 19 0.2239609500651838 1106 20 0.5443303686853724 1106 21 0.43956429894276994 1106 22 0.3146571192582641 1106 23 0.35616396087591795 1106 24 0.3078409521234917 1106 25 0.2931304632507903 1107 1 1.1552053141492955 1107 2 3.47905907847791 1107 3 2.0640857176693927 1107 4 1.2857708807948285 1107 5 1.102491277815112 1107 6 0.9448029960425117 1107 7 0.8673424823117557 1107 8 0.8582050085023454 1107 9 0.7344088898746435 1107 10 0.7281227354089523 1107 11 0.8582664092170447 1107 12 0.7897229750855246 1107 13 0.9025334142421073 1107 14 1.0468158965433076 1107 15 1.0968122479594342 1107 16 0.945690615623981 1107 17 1.0786872693790244 1107 18 0.9983792840801442 1107 19 0.8869776315688469 1107 20 0.9630947841751976 1107 21 0.8773700590690154 1107 22 1.0829023242111262 1107 23 1.152353466399326 1107 24 1.0131033646189422 1107 25 1.0401282478176128 1108 1 1.2009313683980771 1108 2 3.8528622323947026 1108 3 2.1713263936600704 1108 4 1.6200608963348486 1108 5 1.1278431355533343 1108 6 1.043679435259854 1108 7 0.95280727004927 1108 8 1.0035619873820494 1108 9 1.00024204661689 1108 10 0.8968753842752335 1108 11 0.7607179349640205 1108 12 0.8090106949414206 1108 13 0.8529775408226001 1108 14 0.8782676518586996 1108 15 0.9984854484962682 1108 16 0.8785577484911187 1108 17 0.7689575420356647 1108 18 0.8974769036323096 1108 19 0.9654507367464973 1108 20 0.9399737133268573 1108 21 0.7639230893720494 1108 22 0.7685052944731865 1108 23 0.9100382689704958 1108 24 1.1882216806463504 1108 25 1.1593709369466723 1109 1 0.820865092269262 1109 2 2.8418884863312037 1109 3 1.6430224258656558 1109 4 1.1676202018559323 1109 5 0.7503507829596501 1109 6 0.7247589875032787 1109 7 0.6304610516405162 1109 8 0.6113726609136236 1109 9 0.48389443617547556 1109 10 0.6566482306143797 1109 11 0.6495500061933157 1109 12 0.5105964089764683 1109 13 0.6525377888483916 1109 14 0.5701229239108443 1109 15 0.47641345885045183 1109 16 0.4844391282578471 1109 17 0.780179447582988 1109 18 0.6012542753309305 1109 19 0.6460160056151807 1109 20 0.8529194499552801 1109 21 0.6826646450562777 1109 22 0.8247581196895268 1109 23 0.7281553317314504 1109 24 0.6735744222237585 1109 25 0.8774843232708278 1110 1 0.7526614673083287 1110 2 3.4125241813741205 1110 3 1.83269442863239 1110 4 1.1315762283057396 1110 5 0.7685885654091607 1110 6 0.5009099892056668 1110 7 0.4259082912236991 1110 8 0.3106865850990346 1110 9 0.32244269617553556 1110 10 0.42139132200323814 1110 11 0.37239338039236003 1110 12 0.33599169910380056 1110 13 0.3625451811710292 1110 14 0.2818534304698517 1110 15 0.5335151888327658 1110 16 0.4278289328672571 1110 17 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-1.4963412589425147 2315 18 -1.410974176079769 2315 19 -1.537200408133387 2315 20 -1.5330046858490007 2315 21 -1.7781282483782164 2315 22 -1.652296072075984 2315 23 -1.5896293093872131 2315 24 -1.6976968069339193 2315 25 -1.8040379976255139 2316 1 -1.8400915085931793 2316 2 -1.7497927761891603 2316 3 -1.2908606946139756 2316 4 -1.4960568023915464 2316 5 -1.4546138453226227 2316 6 -1.6252429905795651 2316 7 -1.5562437141005283 2316 8 -1.8286774025648498 2316 9 -1.7679018428708702 2316 10 -2.0469741785466624 2316 11 -2.0139496899012315 2316 12 -1.8303952219348503 2316 13 -1.92890851339547 2316 14 -2.2536363685828746 2316 15 -2.8821669250414743 2316 16 -2.526580591429834 2316 17 -2.365146342661106 2316 18 -2.359168602815572 2316 19 -2.4767898509486415 2316 20 -2.5859854833833325 2316 21 -2.6493838935001506 2316 22 -2.6117598071357904 2316 23 -2.5755368498062055 2316 24 -2.4068875140675914 2316 25 -2.5535647439732045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 88528.0 3 6.0 4 781.0 5 3997.0 6 4762.0 7 4168.0 8 3970.0 9 3781.0 10 3710.0 11 3496.0 12 3496.0 13 3325.0 14 3425.0 15 3494.0 16 3650.0 17 4041.0 18 4633.0 19 5483.0 20 7367.0 21 9273.0 22 11852.0 23 13935.0 24 15968.0 25 18050.0 26 20455.0 27 22737.0 28 26255.0 29 31571.0 30 39375.0 31 49201.0 32 62735.0 33 82799.0 34 111334.0 35 157821.0 36 240103.0 37 323462.0 38 747163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26647765024049 19.77187200086721 12.944946318151276 25.01670403074102 2 15.492221347522253 21.778892649604582 41.08431168772124 21.644574315151928 3 18.117074930310316 26.00091019445828 30.28760836593929 25.59440650929211 4 11.55680632015109 17.71477645568035 40.134762980316815 30.59365424385175 5 12.208547936654194 38.75928714706972 36.65554771097319 12.376617205302896 6 29.661359468267783 38.7507563633815 19.255574894576377 12.33230927377434 7 26.52857066762857 32.25541327407413 23.735703452290952 17.480312606006347 8 23.555253195726383 38.31030902690494 21.65748840530006 16.476949372068617 9 26.204909630025575 15.276735560475133 21.297382209716652 37.220972599782634 10 14.240852031724108 29.54151056769408 35.99795720217064 20.219680198411176 11 32.90530520016335 22.630854470746222 26.30518053903323 18.1586597900572 12 22.80952919397328 25.248364406724228 33.211631425444885 18.730474973857607 13 28.483979971927802 22.971073677364128 26.434307885819802 22.110638464888265 14 19.82233452730163 23.50081908156333 28.984834141823995 27.69201224931105 15 21.83032255833795 32.82087391750872 24.10080917595629 21.24799434819704 16 20.553994436039215 29.010205578725746 29.72434377689582 20.711456208339214 17 20.443158365429497 29.666481791510353 29.106529675266085 20.78383016779406 18 20.122212763094325 27.9767050026119 31.941891466319532 19.95919076797424 19 22.505473823303205 27.443175499699556 28.95776348410839 21.093587192888844 20 22.63956990573699 28.0237848930605 29.743405000135503 19.59324020106701 21 21.898505638316067 27.40435608626946 28.38081692270456 22.316321352709913 22 22.085338307569668 29.32600156815542 28.6856558637144 19.903004260560518 23 20.875559004890697 28.984102791017797 29.322671336663568 20.81766686742794 24 22.0236860352758 27.499528073549506 30.221562459699353 20.255223431475343 25 21.51479569737728 28.337670189885632 29.59555327745338 20.55198083528371 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 278.0 1 278.0 2 509.5 3 741.0 4 741.0 5 741.0 6 2020.0 7 3299.0 8 3299.0 9 3299.0 10 4713.5 11 6128.0 12 6128.0 13 6128.0 14 9607.5 15 13087.0 16 13087.0 17 13087.0 18 22993.5 19 32900.0 20 32900.0 21 32900.0 22 51244.5 23 69589.0 24 69589.0 25 69589.0 26 98541.5 27 127494.0 28 127494.0 29 127494.0 30 162565.5 31 197637.0 32 197637.0 33 197637.0 34 219415.0 35 241193.0 36 241193.0 37 241193.0 38 252415.0 39 263637.0 40 263637.0 41 263637.0 42 269528.5 43 275420.0 44 275420.0 45 275420.0 46 273709.0 47 271998.0 48 271998.0 49 271998.0 50 254592.0 51 237186.0 52 237186.0 53 237186.0 54 202606.5 55 168027.0 56 168027.0 57 168027.0 58 139615.5 59 111204.0 60 111204.0 61 111204.0 62 88095.5 63 64987.0 64 64987.0 65 64987.0 66 49002.5 67 33018.0 68 33018.0 69 33018.0 70 23680.5 71 14343.0 72 14343.0 73 14343.0 74 9909.0 75 5475.0 76 5475.0 77 5475.0 78 3636.5 79 1798.0 80 1798.0 81 1798.0 82 1165.5 83 533.0 84 533.0 85 533.0 86 346.0 87 159.0 88 159.0 89 159.0 90 106.0 91 53.0 92 53.0 93 53.0 94 31.5 95 10.0 96 10.0 97 10.0 98 9.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.450466825094081 3 0.0 4 0.0 5 6.074192996735823E-4 6 0.001261563160860517 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.8034736908011485E-4 14 0.0 15 0.0 16 0.0 17 8.877666687536971E-4 18 0.0 19 7.475929842136397E-4 20 9.344912302670495E-4 21 0.0011213894763204594 22 0.005046252643442068 23 7.008684227002871E-4 24 0.0017755333375073941 25 8.877666687536971E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2140202.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.65828539493526 #Duplication Level Percentage of deduplicated Percentage of total 1 87.82933792138144 67.3284645242547 2 9.039919344862483 13.859694341713288 3 1.7641733139362028 4.0571550415755055 4 0.5357681759014501 1.6428427893510895 5 0.2304041957278469 0.8831195296147905 6 0.12527898243134386 0.5762203193525424 7 0.08911948207387484 0.478222268374754 8 0.06146753072265119 0.37695964101271573 9 0.046127685871538274 0.31824623773334737 >10 0.24133925818087626 3.3162761390392537 >50 0.017998621588641225 0.9643910500515851 >100 0.015859452071353468 2.4330354537662506 >500 0.0017263266732548172 0.9630842845006666 >1k 0.0014180540530305602 2.4862386858648686 >5k 6.165452404480697E-5 0.31604969379473763 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6687 0.31244714283978803 No Hit GTCCTACAGTGGACATTTCTAAATT 4822 0.22530583561738565 No Hit CTGTAGGACGTGGAATATGGCAAGA 4502 0.21035397593311286 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4293 0.20058854257682218 No Hit GGTATCAACGCAGAGTACTTTTTTT 4231 0.19769161976299432 No Hit TATCAACGCAGAGTACTTTTTTTTT 4087 0.19096328290507159 No Hit CTTTAGGACGTGAAATATGGCGAGG 4053 0.1893746478136176 No Hit GTCCTACAGTGTGCATTTCTCATTT 2617 0.12227817748044344 No Hit CTGAAGGACCTGGAATATGGCGAGA 2187 0.10218661602970187 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.344912302670496E-5 0.0 10 0.0 0.0 0.0 1.4017368454005742E-4 0.0 11 0.0 0.0 0.0 1.4017368454005742E-4 0.0 12 0.0 0.0 0.0 1.8689824605340992E-4 0.0 13 0.0 0.0 0.0 2.3362280756676238E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1230 0.0 14.700466 1 GCGTCAG 40 0.005485144 14.168328 13 TCGTAAT 40 0.005485994 14.167997 14 AAAATCG 40 0.005485994 14.167997 15 CGGTAGG 120 2.1827873E-11 14.152245 1 TAGGACC 875 0.0 13.385698 4 TAGAACG 85 4.2070315E-6 12.223657 4 GGCGAGG 1355 0.0 11.641382 19 GACCGTC 75 2.1882304E-4 11.334664 7 TGTAGGA 2670 0.0 10.962611 2 GTAGGAC 2870 0.0 10.893652 3 ACTGCGC 70 0.0015650515 10.794917 8 GCGCCAC 70 0.0015650515 10.794917 13 GTATCAA 2590 0.0 10.722664 1 GACGTGG 1345 0.0 10.674528 7 AGGACCT 1755 0.0 10.656521 5 AGGACGT 2995 0.0 10.47053 5 GGACGTG 2915 0.0 10.433609 6 CTGTAGG 2705 0.0 10.340664 1 GGACCTG 1695 0.0 10.309065 6 >>END_MODULE