FastQCFastQC Report
Thu 26 May 2016
SRR1511822_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511822_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140202
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80090.37421701316044No Hit
TATCAACGCAGAGTACTTTTTTTTT53240.2487615654970886No Hit
GTCCTACAGTGGACATTTCTAAATT53090.2480606970743883No Hit
GGTATCAACGCAGAGTACTTTTTTT52390.24478997776845365No Hit
CTGTAGGACGTGGAATATGGCAAGA50860.2376411198569107No Hit
GTCCTAAAGTGTGTATTTCTCATTT48230.225352560178899No Hit
CTTTAGGACGTGAAATATGGCGAGG45840.21418538997720776No Hit
GTCCTACAGTGTGCATTTCTCATTT30100.14064093015519094No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26200.1224183511649835No Hit
CTGAAGGACCTGGAATATGGCGAGA25520.11924108098207553No Hit
CTGTAGGACCTGGAATATGGCGAGA22040.10298093357542885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA250.006043912418.9939510
GGTATCA13450.013.9014881
CCAACGA1052.0012521E-812.66381619
TAGGACC9250.012.3247194
CCGAAGC550.003068360512.0912963
CTACTAG1052.5786903E-711.8108621
TCCAACG1501.7644197E-1011.39743418
GTATCAA30800.010.8094171
TGGCGAA1355.7087163E-810.5531818
GGCGAGG12500.010.4856419
TGTAGGA28950.010.4027512
CTGTAGG28750.010.25293351
AGGACCT19000.010.2003265
CCACCTT7850.010.16236813
GTCCTAC27650.010.1433111
TGTCGAG1502.4541805E-810.13105318
TCCTACA29550.010.09507752
GGACCTG17700.010.0871826
AGGACGT29300.010.083945
GTATTAG2105.456968E-129.9938061
Produced by FastQC (version 0.11.5)