FastQCFastQC Report
Thu 26 May 2016
SRR1511821_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511821_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925110
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53870.2798281656632608No Hit
GGTATCAACGCAGAGTACTTTTTTT32660.1696526432255819No Hit
GTCCTACAGTGGACATTTCTAAATT31320.16269200201546924No Hit
TATCAACGCAGAGTACTTTTTTTTT31240.1622764413462088No Hit
GTCCTAAAGTGTGTATTTCTCATTT29310.15225104020030025No Hit
CTGTAGGACGTGGAATATGGCAAGA29310.15225104020030025No Hit
CTTTAGGACGTGAAATATGGCGAGG25900.13453776667307324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9550.014.2321871
TAGGACC5500.011.1624724
ACCGTGC1053.7182072E-610.7939198
ACTGCGC700.001566137110.7939188
GTATCAA20550.010.6990941
GTCCTAC16050.010.5854661
GGCGAGG8400.010.57014319
CTTAGAC1651.027729E-910.3038213
TAGTACC1401.0545227E-710.1198234
AGGACGT19350.09.8600725
GGACGTG19300.09.8364236
AATCCCG1356.968912E-79.79549919
TGGCGAG17900.09.76227418
TGTAGGA19500.09.7376722
TCCTACA18250.09.6379792
CTATAGT3050.09.6000084
CCCCTAA1251.5129139E-59.5941691
GACGTGG10650.09.57770257
TAGCCCT3950.09.5647284
CTGTAGG19550.09.5594081
Produced by FastQC (version 0.11.5)