FastQCFastQC Report
Thu 26 May 2016
SRR1511821_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511821_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925110
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69100.35894052807372046No Hit
GGTATCAACGCAGAGTACTTTTTTT43980.2284544779259367No Hit
TATCAACGCAGAGTACTTTTTTTTT43790.2274675213364431No Hit
GTCCTACAGTGGACATTTCTAAATT34960.1816000124668201No Hit
CTGTAGGACGTGGAATATGGCAAGA33130.1720940621574871No Hit
GTCCTAAAGTGTGTATTTCTCATTT32470.16866568663608833No Hit
CTTTAGGACGTGAAATATGGCGAGG27790.14435538748435156No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21660.11251305120226897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGG400.0052889114.246266
GGTATCA12050.013.2986741
CAAGACG500.001500980113.2985814
TAGGACC5450.011.5033614
TCCAACG1251.8388164E-811.39730418
TGTACGA600.0058811911.082152
GGCGAGG7900.010.45955119
GTATCAA25900.010.4225211
AGGACCT12150.010.3198865
GTATAAC1303.6337406E-710.2723721
GTCCTAC18600.010.2565931
GTCCTAA9600.010.1347961
CCCGTGC750.002653834410.1306738
TGGTATC3750.09.8789462
AAAGGCG1356.4526284E-79.8508015
CTAGGAC2800.09.8382363
TAGAAAT6650.09.713254
ACTATAC2350.09.7010923
ATTTAGA6600.09.6831571
TGGCGAG18050.09.68192118
Produced by FastQC (version 0.11.5)