FastQCFastQC Report
Thu 26 May 2016
SRR1511820_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511820_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103735
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64110.3047437058374748No Hit
GGTATCAACGCAGAGTACTTTTTTT41190.19579462242155024No Hit
TATCAACGCAGAGTACTTTTTTTTT39070.1857173075506183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT751.0329622E-613.8529486
GACGCTC552.059276E-413.7384618
GGTATCA12800.012.6459711
TAAGACG752.1889746E-411.334234
GTATCAA27050.010.749121
GACGTGA3000.010.7045517
CCCGTAC750.002777311310.0753516
GTCCTAC6550.010.0681521
CGGTTTC856.9551915E-410.00079213
CTACACG951.7432842E-49.9423074
CGTGCGC1151.0997466E-59.85585210
TCCTACA7300.09.7181282
CTAGGGT1857.203198E-109.7004674
TAGGACG5200.09.626834
GTATTGG2403.6379788E-129.5755511
GTCCTAA3550.09.5696861
TTAGGAC4100.09.4451913
GTAGGAC5800.09.4451913
ATTGGAC1304.5633733E-69.4451913
ACCGTCT900.00117436979.4451918
Produced by FastQC (version 0.11.5)