##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511820_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2103735 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.040406705217148 31.0 30.0 34.0 23.0 34.0 2 26.326537325280988 31.0 25.0 34.0 7.0 34.0 3 28.051081528804723 31.0 27.0 34.0 16.0 34.0 4 32.3940676938873 35.0 32.0 37.0 25.0 37.0 5 32.739551797160765 35.0 33.0 37.0 26.0 37.0 6 32.92849954961057 36.0 35.0 37.0 26.0 37.0 7 32.89747425412421 37.0 35.0 37.0 26.0 37.0 8 32.91275849857515 37.0 35.0 37.0 26.0 37.0 9 34.41459404345129 39.0 35.0 39.0 27.0 39.0 10 34.289008834287586 39.0 35.0 39.0 26.0 39.0 11 34.27689894402099 39.0 35.0 39.0 26.0 39.0 12 34.149139981984426 39.0 35.0 39.0 25.0 39.0 13 34.12507183651933 39.0 35.0 39.0 25.0 39.0 14 35.172787447088155 40.0 35.0 41.0 25.0 41.0 15 35.08305038419763 40.0 35.0 41.0 25.0 41.0 16 35.03254117082237 39.0 35.0 41.0 24.0 41.0 17 34.978389388397304 39.0 35.0 41.0 24.0 41.0 18 34.943622652092586 39.0 35.0 41.0 24.0 41.0 19 34.96293497042166 40.0 35.0 41.0 23.0 41.0 20 34.88836141434164 40.0 35.0 41.0 22.0 41.0 21 34.77140514370869 39.0 35.0 41.0 21.0 41.0 22 34.65880921313759 39.0 34.0 41.0 19.0 41.0 23 34.53428830152087 39.0 34.0 41.0 18.0 41.0 24 34.38640798389531 39.0 34.0 41.0 18.0 41.0 25 34.264592736252425 39.0 34.0 41.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0076194983976663 1101 2 3.5564025815871254 1101 3 2.1216643195567464 1101 4 1.2758235732950567 1101 5 0.7063160316519941 1101 6 0.7607711584826262 1101 7 0.6368961714215331 1101 8 0.6978465211052267 1101 9 0.7042305685443679 1101 10 0.6868657267969454 1101 11 0.7560913928215172 1101 12 0.7401325641131962 1101 13 0.6745665578568989 1101 14 0.7653464097713325 1101 15 0.9223129972671629 1101 16 0.7991769017939063 1101 17 0.975034475007341 1101 18 0.9247578056831784 1101 19 1.0078641388035052 1101 20 0.7942023044255109 1101 21 0.8571764311713252 1101 22 0.9800901134704176 1101 23 0.8066798464555589 1101 24 0.8571431268009988 1101 25 0.7950094487894859 1102 1 1.2783622278983735 1102 2 3.9496917485461083 1102 3 2.272358021800926 1102 4 1.5590932419768535 1102 5 1.147351047601397 1102 6 1.040843027583719 1102 7 0.9611101691197561 1102 8 0.9334269468058807 1102 9 1.021126553095911 1102 10 1.0192570931193572 1102 11 0.7928319190872983 1102 12 0.9498238061956883 1102 13 1.1123248777670796 1102 14 1.1633209705028378 1102 15 0.9453416709971592 1102 16 1.2106612345410355 1102 17 1.2476111805493346 1102 18 1.0138559575703 1102 19 1.3065491196161219 1102 20 1.147141358817727 1102 21 1.2777423622140631 1102 22 1.2273774244562077 1102 23 1.491601999994522 1102 24 1.1396452414607268 1102 25 1.1632819859001486 1103 1 1.2557378307492613 1103 2 3.7137212813576 1103 3 2.2594300381111765 1103 4 1.5062801837881565 1103 5 1.0132591877982478 1103 6 0.9501430933673305 1103 7 0.8841444793311979 1103 8 0.70076183646934 1103 9 0.6339284184217036 1103 10 0.9338584438686084 1103 11 1.0438449242631407 1103 12 0.8863006198850343 1103 13 1.0534204501724744 1103 14 1.2200052721720596 1103 15 1.1062904420809758 1103 16 1.0837002841239425 1103 17 1.2636518244908572 1103 18 1.2496442285624525 1103 19 1.2654635935430534 1103 20 1.38230102455298 1103 21 1.3887506450461302 1103 22 1.3371781608466904 1103 23 1.5037262770984796 1103 24 1.5337197216765475 1103 25 1.5091082456506228 1104 1 1.6487278429445702 1104 2 4.030397183669493 1104 3 2.45249334992306 1104 4 1.6508784491902588 1104 5 1.527510560236422 1104 6 1.2824695550612262 1104 7 1.1247183988272482 1104 8 1.245102781358355 1104 9 1.4147380784275256 1104 10 1.4884908784964708 1104 11 1.4236705488935186 1104 12 1.3879066107579803 1104 13 1.4560072825377972 1104 14 1.6615396455401878 1104 15 1.6634822416797235 1104 16 1.6303613189101256 1104 17 1.3483349726588187 1104 18 1.3275598046511234 1104 19 1.4822042252186023 1104 20 1.422625927631934 1104 21 1.4795045664691528 1104 22 1.4292445020566689 1104 23 1.7089385878143304 1104 24 1.537104705422344 1104 25 1.652330745918114 1105 1 1.467813405776642 1105 2 3.9945027142994007 1105 3 2.4662337419547633 1105 4 1.614591170682445 1105 5 1.2481485886618913 1105 6 1.219801664957771 1105 7 0.9579113335820537 1105 8 1.0140888120833935 1105 9 1.1080380229841538 1105 10 1.0207672074495733 1105 11 1.1647942387625179 1105 12 0.8638629066496506 1105 13 1.0494830705599796 1105 14 1.0898734596555357 1105 15 1.2251420239570692 1105 16 1.2679624110013634 1105 17 1.3960403126126195 1105 18 1.2127206722683042 1105 19 1.1413039455466745 1105 20 1.3290913205331378 1105 21 1.2797912966808838 1105 22 1.3098714418075232 1105 23 1.3704879948157398 1105 24 1.423905858071656 1105 25 1.391734361398882 1106 1 0.9058958831465276 1106 2 3.7693985839604593 1106 3 2.1716603089546034 1106 4 1.324008303989018 1106 5 1.0817122260623933 1106 6 0.9800183920518322 1106 7 0.7849740903436739 1106 8 0.6354253323863404 1106 9 0.5614628638001804 1106 10 0.8189139177204794 1106 11 0.6769704696280883 1106 12 0.5583080822181188 1106 13 0.6264643983835612 1106 14 0.8217534646564246 1106 15 0.8194537328351856 1106 16 0.7074930622793261 1106 17 0.6700390930977491 1106 18 0.677382980704671 1106 19 0.524892093880041 1106 20 0.6815453748717388 1106 21 0.749460799377637 1106 22 0.5796320791137077 1106 23 0.4797733064610128 1106 24 0.5320755658486931 1106 25 0.587908736671686 1107 1 0.9578576973979871 1107 2 3.3252083686880454 1107 3 1.8194827022755717 1107 4 1.091206561698904 1107 5 0.7538804455418031 1107 6 0.6527227919918062 1107 7 0.6480346784662814 1107 8 0.5998905785969058 1107 9 0.40661367735298626 1107 10 0.5719987740712682 1107 11 0.4482078636617217 1107 12 0.5686172966814098 1107 13 0.4916815635947742 1107 14 0.7694521592590817 1107 15 0.6592613329641921 1107 16 0.6197372954429241 1107 17 0.6238033628401709 1107 18 0.4390782486022573 1107 19 0.661053639933499 1107 20 0.6470013424659555 1107 21 0.6467970602383204 1107 22 0.8353715676191058 1107 23 0.8423889186888331 1107 24 0.8216173255498589 1107 25 1.1035171115410378 1108 1 1.1410476963222855 1108 2 3.7466145450811723 1108 3 2.234451349225953 1108 4 1.471190500331872 1108 5 1.0069846849266497 1108 6 0.8847886806898657 1108 7 0.8767577719671777 1108 8 0.8180112688276608 1108 9 0.9394954315658524 1108 10 1.0203255913611642 1108 11 0.9360106834887958 1108 12 0.778176174759686 1108 13 0.78418692356599 1108 14 0.8471884338379851 1108 15 0.9523780356731066 1108 16 0.9375580869381892 1108 17 1.0241806936857856 1108 18 0.9836181720418367 1108 19 0.9740031634710036 1108 20 0.9108299291027535 1108 21 0.9110777205719387 1108 22 1.0415802318549297 1108 23 1.0693718762059632 1108 24 1.1021941994069238 1108 25 1.1423194083536217 1109 1 0.5599444066748767 1109 2 2.526527300629933 1109 3 1.3752663604138107 1109 4 0.8035150733170084 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-1.8348194686607684 2315 15 -1.8765675021098005 2315 16 -1.8066948076668439 2315 17 -1.8398823417758692 2315 18 -1.966138316472005 2315 19 -1.9614144856381301 2315 20 -1.9056231509093138 2315 21 -2.0084340628796795 2315 22 -2.034960945787269 2315 23 -1.9787543192007462 2315 24 -2.2159443491459925 2315 25 -2.117079820142237 2316 1 -1.5959392811912139 2316 2 -1.5909148661696477 2316 3 -1.1135933121952206 2316 4 -1.324120297965191 2316 5 -1.300873215051805 2316 6 -1.4694938595103615 2316 7 -1.5476301973752342 2316 8 -1.8502698619621292 2316 9 -1.8153814488614017 2316 10 -1.9257928515315115 2316 11 -1.941348768588341 2316 12 -1.6660483728603381 2316 13 -1.7626399373508264 2316 14 -1.8990769197085484 2316 15 -2.4396659297788617 2316 16 -2.1774493677946936 2316 17 -1.888250218994827 2316 18 -2.002031831161368 2316 19 -2.041453031237296 2316 20 -2.1606383183943763 2316 21 -2.2839112283689644 2316 22 -2.3440362104902803 2316 23 -2.1185518205261857 2316 24 -2.045718184185681 2316 25 -2.2472238727771696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96010.0 3 2.0 4 917.0 5 4373.0 6 5101.0 7 4569.0 8 4210.0 9 4030.0 10 4047.0 11 3854.0 12 3508.0 13 3698.0 14 3689.0 15 3817.0 16 3967.0 17 4202.0 18 4848.0 19 5916.0 20 7658.0 21 9737.0 22 11931.0 23 14521.0 24 16875.0 25 18915.0 26 20980.0 27 24064.0 28 27315.0 29 32786.0 30 40715.0 31 51187.0 32 64126.0 33 83556.0 34 111790.0 35 157509.0 36 234571.0 37 316167.0 38 698574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01333818185275 18.453322305328378 12.709347897905394 24.823991614913478 2 16.93171526715139 21.258515288673756 38.23810273308898 23.57166671108588 3 18.598635284387054 24.87447325827635 29.72479898846575 26.802092468870843 4 12.668468224372367 16.795604009060078 37.851392879806625 32.68453488676093 5 13.680949137266776 37.50582895735699 35.10099485625708 13.712227049119155 6 31.82009185641802 36.685525341324265 18.077375389667246 13.41700741259047 7 27.949242656513295 31.804766284726927 22.170615595595454 18.075375463164324 8 25.850071420592425 35.17139753818803 20.708454249228158 18.27007679199139 9 26.706453046605205 15.206430467715753 20.01112307396131 38.07599341171773 10 15.544353257420731 28.487285708513667 33.82811998659527 22.140241047470333 11 34.216762092183664 22.74511761224679 23.96433010811723 19.073790187452317 12 23.07538734679035 24.869719807865533 31.049300410935786 21.005592434408328 13 28.72167884821612 21.676423948343132 25.845499037898435 23.756398165542315 14 21.867250390377116 21.42689074431903 27.591402909587 29.114455955716856 15 23.758505705328854 29.004508647714662 23.905197185006667 23.331788461949817 16 23.15486503765921 27.774695957428097 26.283491029050708 22.786947975861978 17 22.36096058410815 27.7227482744852 27.07009487954671 22.846196261859944 18 22.256035099477835 27.164733200712064 28.29035025799352 22.28888144181658 19 23.379093324713722 26.84959571808169 27.71477328364381 22.05653767356077 20 23.771453017861173 26.487207215772024 27.8055829540458 21.935756812321003 21 23.932770456876824 26.258319793812667 27.26035523772673 22.548554511583777 22 23.73058531764424 26.527944355154215 27.39188078494904 22.3495895422525 23 23.443751547846706 26.436532462118457 27.200651794979297 22.91906419505554 24 23.199020006769064 26.4201250375531 27.66123113313381 22.719623822544026 25 23.31774979025201 26.49698271866674 27.436606194280117 22.748661296801135 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 449.0 1 449.0 2 573.5 3 698.0 4 698.0 5 698.0 6 1762.0 7 2826.0 8 2826.0 9 2826.0 10 3836.0 11 4846.0 12 4846.0 13 4846.0 14 7373.0 15 9900.0 16 9900.0 17 9900.0 18 17473.0 19 25046.0 20 25046.0 21 25046.0 22 37834.0 23 50622.0 24 50622.0 25 50622.0 26 70644.5 27 90667.0 28 90667.0 29 90667.0 30 114368.0 31 138069.0 32 138069.0 33 138069.0 34 159420.5 35 180772.0 36 180772.0 37 180772.0 38 198832.0 39 216892.0 40 216892.0 41 216892.0 42 233072.5 43 249253.0 44 249253.0 45 249253.0 46 260141.5 47 271030.0 48 271030.0 49 271030.0 50 268722.5 51 266415.0 52 266415.0 53 266415.0 54 244320.5 55 222226.0 56 222226.0 57 222226.0 58 193009.0 59 163792.0 60 163792.0 61 163792.0 62 135181.5 63 106571.0 64 106571.0 65 106571.0 66 82630.0 67 58689.0 68 58689.0 69 58689.0 70 43156.5 71 27624.0 72 27624.0 73 27624.0 74 19553.5 75 11483.0 76 11483.0 77 11483.0 78 7798.5 79 4114.0 80 4114.0 81 4114.0 82 2687.0 83 1260.0 84 1260.0 85 1260.0 86 813.0 87 366.0 88 366.0 89 366.0 90 230.0 91 94.0 92 94.0 93 94.0 94 59.0 95 24.0 96 24.0 97 24.0 98 15.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.471506629874961 3 0.0 4 0.0 5 6.654830575143733E-4 6 8.080865698388818E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 3.3274152875718664E-4 14 0.0 15 0.0 16 0.0 17 7.130175616225428E-4 18 0.0 19 2.376725205408476E-4 20 4.753450410816952E-4 21 8.080865698388818E-4 22 0.005133726443682308 23 2.8520702464901714E-4 24 0.0022341216930839673 25 9.506900821633904E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2103735.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.54103053336702 #Duplication Level Percentage of deduplicated Percentage of total 1 81.50207256193136 58.30742261685826 2 12.308706153089114 17.611550454487816 3 3.345472564649568 7.180156646884095 4 1.2420808304458393 3.554389704633426 5 0.5393876632483832 1.9294174642887032 6 0.2843648409860219 1.2206252262958233 7 0.17426476737874713 0.8726956730753136 8 0.12569177937135276 0.7193695540639343 9 0.08733006077905439 0.5622914290207617 >10 0.36261100935197405 4.394388223586374 >50 0.0172070572927857 0.8474269236413595 >100 0.009200253210917126 1.2314855810735572 >500 0.0010736388433086662 0.5290235666036646 >1k 4.697169939474232E-4 0.7319919549520086 >5k 6.710242770677475E-5 0.3077649805349769 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6411 0.3047437058374748 No Hit GGTATCAACGCAGAGTACTTTTTTT 4119 0.19579462242155024 No Hit TATCAACGCAGAGTACTTTTTTTTT 3907 0.1857173075506183 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.753450410816952E-5 0.0 5 0.0 0.0 0.0 4.753450410816952E-5 0.0 6 0.0 0.0 0.0 4.753450410816952E-5 9.506900821633903E-5 7 0.0 0.0 0.0 4.753450410816952E-5 9.506900821633903E-5 8 0.0 0.0 0.0 4.753450410816952E-5 9.506900821633903E-5 9 0.0 0.0 0.0 9.506900821633903E-5 9.506900821633903E-5 10 0.0 0.0 0.0 9.506900821633903E-5 9.506900821633903E-5 11 0.0 0.0 0.0 9.506900821633903E-5 9.506900821633903E-5 12 0.0 0.0 0.0 9.506900821633903E-5 9.506900821633903E-5 13 0.0 0.0 0.0 9.506900821633903E-5 9.506900821633903E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 75 1.0329622E-6 13.852948 6 GACGCTC 55 2.059276E-4 13.738461 8 GGTATCA 1280 0.0 12.645971 1 TAAGACG 75 2.1889746E-4 11.33423 4 GTATCAA 2705 0.0 10.74912 1 GACGTGA 300 0.0 10.704551 7 CCCGTAC 75 0.0027773113 10.07535 16 GTCCTAC 655 0.0 10.068152 1 CGGTTTC 85 6.9551915E-4 10.000792 13 CTACACG 95 1.7432842E-4 9.942307 4 CGTGCGC 115 1.0997466E-5 9.855852 10 TCCTACA 730 0.0 9.718128 2 CTAGGGT 185 7.203198E-10 9.700467 4 TAGGACG 520 0.0 9.62683 4 GTATTGG 240 3.6379788E-12 9.575551 1 GTCCTAA 355 0.0 9.569686 1 TTAGGAC 410 0.0 9.445191 3 GTAGGAC 580 0.0 9.445191 3 ATTGGAC 130 4.5633733E-6 9.445191 3 ACCGTCT 90 0.0011743697 9.445191 8 >>END_MODULE