FastQCFastQC Report
Thu 26 May 2016
SRR1511820_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511820_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103735
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84270.4005732661195445No Hit
GGTATCAACGCAGAGTACTTTTTTT55940.2659080159811003No Hit
TATCAACGCAGAGTACTTTTTTTTT55350.26310348023871827No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25800.12263902059907736No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21660.10295973589829518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGC400.005290099414.24586511
GGATCGA500.001503139313.2961416
CAACCGT655.4691467E-513.1500296
GGTATCA16100.013.0907261
ACACCGT853.9580063E-612.290556
GCTTGCG658.045053E-411.68891416
GTGGTAT5500.011.2706241
AAGGCGT1201.2810597E-711.0801166
GTCCTAC5750.010.9464531
GCGCCAC1502.1445885E-910.76354213
TACACCG1002.3983534E-510.4504445
TGCACCG951.6437446E-410.0004255
TGTGTCG1252.5801673E-69.87713210
GTATTAG1659.598807E-99.8256731
GGCGTGC1651.0271833E-89.7850388
GTATCAA31650.09.76267051
TAGGACC2054.1836756E-119.7321214
TAGGACA4100.09.7321214
CTAGACT2151.0913936E-119.7213444
TAAGACC2350.09.702544
Produced by FastQC (version 0.11.5)