FastQCFastQC Report
Thu 26 May 2016
SRR1511819_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511819_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990977
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46270.23239846567790584No Hit
GTCCTACAGTGGACATTTCTAAATT45430.22817943150523584No Hit
CTGTAGGACGTGGAATATGGCAAGA43200.2169789003087429No Hit
GTCCTAAAGTGTGTATTTCTCATTT40850.20517564994472562No Hit
CTTTAGGACGTGAAATATGGCGAGG35000.1757930905279167No Hit
GGTATCAACGCAGAGTACTTTTTTT30270.1520359100080011No Hit
TATCAACGCAGAGTACTTTTTTTTT27850.139881073462928No Hit
GTCCTACAGTGTGCATTTCTCATTT23910.12009179412921395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10450.013.0965191
TACACCG655.7562385E-513.0784245
TAGGACC8000.012.6333944
CGAGCAT658.421665E-411.62526610
TCCAACG1800.011.54453518
TAGTACC1407.4942363E-1011.469574
GTATTAG2400.011.2383921
CTGTCCG600.006133556411.0197849
GTCTTAC2100.010.941081
CTAGCAC1409.138603E-910.7948893
CGTTAGG650.007332852610.7581181
AGGACCT16200.010.7282545
TCTATAC2650.010.6930523
GTCCTAA12650.010.6609251
GGACCTG15400.010.6108856
TCGAACT1252.425386E-710.57899219
GGCGAGG11700.010.57576319
TTAGGAC16200.010.4950313
CTCGAAC1201.6406739E-610.232656518
GTATCAA20900.010.1808711
Produced by FastQC (version 0.11.5)