##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511819_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1990977 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.091126617735917 31.0 30.0 34.0 23.0 34.0 2 26.388343511753273 31.0 26.0 34.0 7.0 34.0 3 28.125023543717482 31.0 27.0 34.0 16.0 34.0 4 32.44522814678422 35.0 32.0 37.0 25.0 37.0 5 32.807912899044034 35.0 33.0 37.0 26.0 37.0 6 33.00132547990258 37.0 35.0 37.0 26.0 37.0 7 32.97127490674177 37.0 35.0 37.0 26.0 37.0 8 32.97929308073373 37.0 35.0 37.0 26.0 37.0 9 34.488342155635145 39.0 35.0 39.0 27.0 39.0 10 34.354753972547144 39.0 35.0 39.0 26.0 39.0 11 34.348801618501874 39.0 35.0 39.0 26.0 39.0 12 34.22875452604425 39.0 35.0 39.0 25.0 39.0 13 34.22435618291924 39.0 35.0 39.0 25.0 39.0 14 35.28041911081846 40.0 36.0 41.0 25.0 41.0 15 35.22352342593611 40.0 36.0 41.0 25.0 41.0 16 35.17389452515022 40.0 36.0 41.0 25.0 41.0 17 35.1255001941258 40.0 36.0 41.0 25.0 41.0 18 35.08669562732267 40.0 36.0 41.0 25.0 41.0 19 35.10932923886112 40.0 35.0 41.0 24.0 41.0 20 35.038359056885135 40.0 35.0 41.0 24.0 41.0 21 34.92708403964486 40.0 35.0 41.0 22.0 41.0 22 34.82605022559276 40.0 35.0 41.0 21.0 41.0 23 34.70998911589636 39.0 35.0 41.0 19.0 41.0 24 34.585006255722696 39.0 35.0 41.0 18.0 41.0 25 34.48097893647189 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9864976375465844 1101 2 3.5731483136419406 1101 3 2.10246957606185 1101 4 1.2694348320339017 1101 5 0.7296130458911279 1101 6 0.7911908941469719 1101 7 0.6549480156892358 1101 8 0.5628326657252103 1101 9 0.5991142723706417 1101 10 0.7205938633518514 1101 11 0.6484584211928563 1101 12 0.7230000772853558 1101 13 0.6143528309665172 1101 14 0.5789346792150951 1101 15 0.7984775293181272 1101 16 0.7535958063564792 1101 17 0.7162560240863556 1101 18 0.6947214903099024 1101 19 0.6802725486059842 1101 20 0.7045427025513646 1101 21 0.9088315554968176 1101 22 0.8043292453986055 1101 23 0.6203234230386272 1101 24 0.5596223089238208 1101 25 0.5926112178578293 1102 1 1.521338666947944 1102 2 4.185471027494863 1102 3 2.3773732546086954 1102 4 1.5647631611541755 1102 5 1.3756543788899833 1102 6 1.2555030721374365 1102 7 1.050351600969904 1102 8 0.9905253247118893 1102 9 1.1179842721776723 1102 10 1.0148232595658868 1102 11 1.0826163562152686 1102 12 1.0304916029271212 1102 13 1.1522755036536765 1102 14 1.2959592950549066 1102 15 1.1843139340033062 1102 16 1.3015928347029657 1102 17 1.3507625816637443 1102 18 1.2924448306542757 1102 19 1.3353169626049208 1102 20 1.495674375103448 1102 21 1.3322336485185744 1102 22 1.565407338137348 1102 23 1.699323738213998 1102 24 1.4289676446499158 1102 25 1.4131493958868901 1103 1 1.3495263542113527 1103 2 3.918632172372643 1103 3 2.475520674275117 1103 4 1.6179079552082882 1103 5 1.1925734798655583 1103 6 1.2940303160201907 1103 7 1.122058519843101 1103 8 1.0250704412268945 1103 9 1.1492185931475447 1103 10 1.3306219351245474 1103 11 1.4570923880378146 1103 12 1.1143585234747988 1103 13 1.2915160645312511 1103 14 1.3654336739421566 1103 15 1.3792559519879788 1103 16 1.1704217993005983 1103 17 1.2859764444042483 1103 18 1.4335420965084467 1103 19 1.623745861454509 1103 20 1.5069989826621324 1103 21 1.6695614215868702 1103 22 1.701827443494274 1103 23 1.6291774118857916 1103 24 1.7559634947665401 1103 25 1.6906442970278164 1104 1 1.4039794516022042 1104 2 3.887887208868822 1104 3 2.2493494445587636 1104 4 1.5511593379773032 1104 5 1.1627378388841692 1104 6 1.0458204897013772 1104 7 0.92527149855826 1104 8 1.0506324424643765 1104 9 1.1011530608739335 1104 10 1.1279780314599037 1104 11 1.0658224712916038 1104 12 1.0899623805520733 1104 13 1.253716660257851 1104 14 1.2916470530175204 1104 15 1.0857185406161065 1104 16 1.245015598061478 1104 17 1.0541232697591383 1104 18 1.2136551131462952 1104 19 1.1609899273765762 1104 20 1.20619814484089 1104 21 1.3161525481488354 1104 22 1.2494714821777606 1104 23 1.4172676936994577 1104 24 1.3356469166839773 1104 25 1.3306324153700615 1105 1 1.620099190204968 1105 2 4.237730705193936 1105 3 2.5657596929331454 1105 4 1.795966846571993 1105 5 1.5228213709367822 1105 6 1.3060824913478442 1105 7 1.1949729691275692 1105 8 1.228787797755416 1105 9 1.3638539790773265 1105 10 1.2089914186381847 1105 11 1.4057838224670434 1105 12 1.1271035315886166 1105 13 1.2326875807389825 1105 14 1.344668304274613 1105 15 1.5884202087387749 1105 16 1.5136422776148137 1105 17 1.6612867336922221 1105 18 1.579769619103395 1105 19 1.4546675482744789 1105 20 1.4578439098396956 1105 21 1.6481398510082101 1105 22 1.4919836221955265 1105 23 1.4543836608798557 1105 24 1.5445251773637452 1105 25 1.740826504551137 1106 1 1.2199961144100406 1106 2 3.878151965221992 1106 3 2.2109445314862555 1106 4 1.3365804807321595 1106 5 1.1416060190668134 1106 6 0.9239909652047444 1106 7 1.0780575828289614 1106 8 0.8111003035147846 1106 9 0.9001110550325393 1106 10 0.9964529715767867 1106 11 0.9543430575810135 1106 12 1.09550992997206 1106 13 0.848832299875454 1106 14 1.0101573334861271 1106 15 1.1769430761669142 1106 16 1.0340144550744768 1106 17 1.1503867835492088 1106 18 1.1073793142313662 1106 19 0.9749655856020851 1106 20 1.0744079559578594 1106 21 1.179889987357015 1106 22 1.1523218632854224 1106 23 1.1006022590070188 1106 24 1.1848599905981487 1106 25 1.2988810794925527 1107 1 0.9036852288984143 1107 2 3.4600217131578503 1107 3 1.9033196596670443 1107 4 1.137228707222448 1107 5 0.8287185287549832 1107 6 0.7511814227890596 1107 7 0.7175817547708618 1107 8 0.7759212638473088 1107 9 0.6970761764643711 1107 10 0.6770133392700473 1107 11 0.6591388388680457 1107 12 0.5895954164418171 1107 13 0.7392367775995083 1107 14 0.826223649175688 1107 15 0.8684182456334213 1107 16 0.8993196636546799 1107 17 0.7954868562796165 1107 18 0.8704508433343037 1107 19 0.9421018069168099 1107 20 0.8715198192829234 1107 21 0.9237881158737977 1107 22 0.9491585855606672 1107 23 1.1023500597711262 1107 24 1.1205576347502273 1107 25 0.9674752863322382 1108 1 0.9213746992668526 1108 2 3.5331961149547446 1108 3 1.8827625034304099 1108 4 1.2125478059336885 1108 5 0.8530027824643227 1108 6 0.6575489883294097 1108 7 0.6297830492021106 1108 8 0.5199693786344497 1108 9 0.594320285637238 1108 10 0.6053173604681845 1108 11 0.6955629201738986 1108 12 0.5547036629611242 1108 13 0.4977239781402929 1108 14 0.6368983894744815 1108 15 0.6495937592493704 1108 16 0.5868982535758605 1108 17 0.5848099189119154 1108 18 0.6488879404851104 1108 19 0.6605418062423567 1108 20 0.6007760399258686 1108 21 0.5865462139887683 1108 22 0.49527557283759904 1108 23 0.5641297468982955 1108 24 0.6408340260185597 1108 25 0.6854730616722335 1109 1 0.6365682162923001 1109 2 2.7180599221651676 1109 3 1.5975122593985418 1109 4 1.047259662308825 1109 5 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-1.4759465584501754 2315 21 -1.7942987942219446 2315 22 -1.6827904300577856 2315 23 -1.7852453194987419 2315 24 -1.776829146199681 2315 25 -1.8577901459158568 2316 1 -1.626417968408827 2316 2 -1.6427044982071664 2316 3 -1.2790200482535106 2316 4 -1.4327810902063192 2316 5 -1.587434512544423 2316 6 -1.7217459260335453 2316 7 -1.805260847999726 2316 8 -1.9004358677932665 2316 9 -1.867577465652964 2316 10 -2.258357768214914 2316 11 -2.234929876269156 2316 12 -2.040307991834446 2316 13 -1.9915707592517933 2316 14 -2.339341375710461 2316 15 -2.994099420462014 2316 16 -2.5920388846872626 2316 17 -2.6322204088381227 2316 18 -2.6265804845415985 2316 19 -2.4517561098835614 2316 20 -2.7886523526056237 2316 21 -2.679005943538904 2316 22 -2.7347791785773268 2316 23 -2.739854583595637 2316 24 -2.614999692156175 2316 25 -2.8698396037112524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91011.0 3 3.0 4 788.0 5 4044.0 6 4736.0 7 4305.0 8 3908.0 9 3664.0 10 3611.0 11 3377.0 12 3373.0 13 3300.0 14 3282.0 15 3571.0 16 3563.0 17 3899.0 18 4341.0 19 5454.0 20 7081.0 21 9114.0 22 11251.0 23 13185.0 24 15279.0 25 17367.0 26 19386.0 27 21782.0 28 24687.0 29 29591.0 30 36754.0 31 45588.0 32 58010.0 33 75955.0 34 102737.0 35 144277.0 36 220586.0 37 297004.0 38 691113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.567141659597276 19.424232424583508 12.79331704987049 25.215308865948728 2 15.603383524547231 21.62340759848162 40.77604972871672 21.99715914825443 3 18.374094728367027 25.545197156973686 30.171167220917166 25.90954089374212 4 11.715303592156012 17.439327526134154 39.85555835150281 30.98981053020703 5 12.334163416150753 38.51602689347784 36.69992355469656 12.449886135674843 6 30.196638807409492 38.51785068509664 18.92951139148953 12.35599911600434 7 26.90010984556828 31.977667245779333 23.411470850743125 17.710752057909257 8 23.773102351257698 38.32319509466961 21.441031212314357 16.46267134175834 9 26.189755080043614 15.236087609249127 21.04680265015618 37.527354660551076 10 14.220204452386945 29.4640269576193 35.934267447589804 20.381501142403955 11 33.42821137562112 22.45390077333892 26.051029218318444 18.066858632721523 12 22.9173415865678 24.962267268783116 33.11896621608386 19.00142492856522 13 28.761766231887627 22.687399577995283 26.260778021600494 22.2900561685166 14 20.062210663407964 23.123421315263812 28.62639799455242 28.1879700267758 15 21.99457854108812 32.58967833380295 23.81805515583555 21.597687969273377 16 20.82088341552916 28.845637091739384 29.418119847692864 20.915359645038592 17 20.721982276918254 29.4664943547419 28.885268083836817 20.926255284503025 18 20.463521175784553 27.898865732753315 31.522714727493085 20.114898363969047 19 22.813263290387745 27.333122715622395 28.417720215633697 21.435893778356167 20 22.812578636569338 27.98063047881854 29.26262316276094 19.944167721851187 21 22.083768634226704 27.307982078997068 27.961536143368022 22.646713143408206 22 22.277775517471913 29.182507643600996 28.290741636491585 20.248975202435503 23 21.27967774501876 28.736344595850266 28.908773110594332 21.075204548536643 24 22.344409552912907 27.400031743926966 29.752648508336797 20.50291019482333 25 21.91766785872142 28.16254470205328 29.16688431711335 20.752903122111945 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 206.0 1 206.0 2 360.5 3 515.0 4 515.0 5 515.0 6 1373.5 7 2232.0 8 2232.0 9 2232.0 10 3324.0 11 4416.0 12 4416.0 13 4416.0 14 7425.0 15 10434.0 16 10434.0 17 10434.0 18 18815.5 19 27197.0 20 27197.0 21 27197.0 22 43340.0 23 59483.0 24 59483.0 25 59483.0 26 85664.5 27 111846.0 28 111846.0 29 111846.0 30 145312.5 31 178779.0 32 178779.0 33 178779.0 34 199136.0 35 219493.0 36 219493.0 37 219493.0 38 231845.5 39 244198.0 40 244198.0 41 244198.0 42 250964.0 43 257730.0 44 257730.0 45 257730.0 46 257254.0 47 256778.0 48 256778.0 49 256778.0 50 241214.5 51 225651.0 52 225651.0 53 225651.0 54 194021.0 55 162391.0 56 162391.0 57 162391.0 58 135558.0 59 108725.0 60 108725.0 61 108725.0 62 86609.0 63 64493.0 64 64493.0 65 64493.0 66 48709.5 67 32926.0 68 32926.0 69 32926.0 70 23830.5 71 14735.0 72 14735.0 73 14735.0 74 10245.5 75 5756.0 76 5756.0 77 5756.0 78 3893.5 79 2031.0 80 2031.0 81 2031.0 82 1361.0 83 691.0 84 691.0 85 691.0 86 447.0 87 203.0 88 203.0 89 203.0 90 125.5 91 48.0 92 48.0 93 48.0 94 31.0 95 14.0 96 14.0 97 14.0 98 10.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.446722890319677 3 0.0 4 0.0 5 5.524925702305953E-4 6 8.538521539927382E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.009063891747619E-4 14 0.0 15 0.0 16 0.0 17 7.031723621116668E-4 18 0.0 19 4.018127783495238E-4 20 7.031723621116668E-4 21 8.538521539927382E-4 22 0.004821753340194287 23 3.515861810558334E-4 24 0.002260196878216072 25 8.538521539927382E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1990977.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.48117032605334 #Duplication Level Percentage of deduplicated Percentage of total 1 88.20681069088795 68.34366923058633 2 8.774088847969953 13.596533449709703 3 1.6685070604097332 3.8783363921348735 4 0.5066716976001366 1.5703006440458713 5 0.24456128832096496 0.9474447417777867 6 0.1414024495804241 0.6573616368277208 7 0.08670831876800202 0.4702783410604504 8 0.05789601242455478 0.3588680639892984 9 0.04810360019539763 0.335441091603238 >10 0.2293408458095876 3.1397787532447436 >50 0.01833455284611602 0.9910371541779077 >100 0.014231731634769318 2.218096428366859 >500 0.0020319609828484443 1.1519435270357656 >1k 0.0013109425695796203 2.340910545439509 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4627 0.23239846567790584 No Hit GTCCTACAGTGGACATTTCTAAATT 4543 0.22817943150523584 No Hit CTGTAGGACGTGGAATATGGCAAGA 4320 0.2169789003087429 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4085 0.20517564994472562 No Hit CTTTAGGACGTGAAATATGGCGAGG 3500 0.1757930905279167 No Hit GGTATCAACGCAGAGTACTTTTTTT 3027 0.1520359100080011 No Hit TATCAACGCAGAGTACTTTTTTTTT 2785 0.139881073462928 No Hit GTCCTACAGTGTGCATTTCTCATTT 2391 0.12009179412921395 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.022659729369048E-5 0.0 8 0.0 0.0 0.0 5.022659729369048E-5 0.0 9 0.0 0.0 0.0 5.022659729369048E-5 0.0 10 0.0 0.0 0.0 5.022659729369048E-5 0.0 11 0.0 0.0 0.0 5.022659729369048E-5 0.0 12 0.0 0.0 0.0 5.022659729369048E-5 5.022659729369048E-5 13 0.0 5.022659729369048E-5 0.0 5.022659729369048E-5 5.022659729369048E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1045 0.0 13.096519 1 TACACCG 65 5.7562385E-5 13.078424 5 TAGGACC 800 0.0 12.633394 4 CGAGCAT 65 8.421665E-4 11.625266 10 TCCAACG 180 0.0 11.544535 18 TAGTACC 140 7.4942363E-10 11.46957 4 GTATTAG 240 0.0 11.238392 1 CTGTCCG 60 0.0061335564 11.019784 9 GTCTTAC 210 0.0 10.94108 1 CTAGCAC 140 9.138603E-9 10.794889 3 CGTTAGG 65 0.0073328526 10.758118 1 AGGACCT 1620 0.0 10.728254 5 TCTATAC 265 0.0 10.693052 3 GTCCTAA 1265 0.0 10.660925 1 GGACCTG 1540 0.0 10.610885 6 TCGAACT 125 2.425386E-7 10.578992 19 GGCGAGG 1170 0.0 10.575763 19 TTAGGAC 1620 0.0 10.495031 3 CTCGAAC 120 1.6406739E-6 10.2326565 18 GTATCAA 2090 0.0 10.180871 1 >>END_MODULE