FastQCFastQC Report
Thu 26 May 2016
SRR1511819_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511819_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990977
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54580.27413676802896264No Hit
GTCCTACAGTGGACATTTCTAAATT51830.26032445377319774No Hit
CTGTAGGACGTGGAATATGGCAAGA49030.24626100653096444No Hit
GTCCTAAAGTGTGTATTTCTCATTT45450.22827988469982324No Hit
CTTTAGGACGTGAAATATGGCGAGG41400.2079381127958786No Hit
GGTATCAACGCAGAGTACTTTTTTT34900.1752908245549798No Hit
TATCAACGCAGAGTACTTTTTTTTT33830.16991657864455492No Hit
GTCCTACAGTGTGCATTTCTCATTT28290.1420910437438504No Hit
CTGAAGGACCTGGAATATGGCGAGA23240.11672661211053667No Hit
CTGTAGGACCTGGAATATGGCGAGA22000.11049851404611906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC456.780955E-414.77275810
GGTATCA10950.012.80666351
GGCGTGC751.4842526E-512.6623648
TAGGACC8950.012.4207034
AGGCGTG1353.7289283E-1011.95889957
TCCAACG1900.011.49638218
AAAGGCG1251.828812E-811.4015685
GGACGTA600.0058915211.0795696
GTCGAGG700.001495641510.85372719
TGCGCCA1053.4813474E-610.85345512
GCGTGCG700.001495954910.8534559
TTAGACT2550.010.8054084
AAGGCGT1158.8580236E-710.7354836
GTAGGAC26250.010.7138553
TGTAGGA27800.010.6975142
AGTCGTC1701.4006218E-1010.614048
GGCGAGG11600.010.47946119
CTGTAGG27350.010.4639741
GTGCGCC1002.408315E-510.4464511
CCAACGA1553.6816346E-910.41607819
Produced by FastQC (version 0.11.5)