FastQCFastQC Report
Thu 26 May 2016
SRR1511818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975961
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64550.32667648804809407No Hit
GGTATCAACGCAGAGTACTTTTTTT41030.2076457986772006No Hit
TATCAACGCAGAGTACTTTTTTTTT39270.19873874028890248No Hit
GTCCTACAGTGGACATTTCTAAATT34410.17414311314848827No Hit
CTGTAGGACGTGGAATATGGCAAGA31070.15723994552524062No Hit
GTCCTAAAGTGTGTATTTCTCATTT27180.13755332215564983No Hit
ATTCCATTCCATTCCATTCCATTCC25480.12894991348513457No Hit
CTTTAGGACGTGAAATATGGCGAGG25110.1270774068921401No Hit
GAATGGAATGGAATGGAATGGAATG20150.1019756968887544No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20050.1014696140257829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG250.00450042119.9877621
GGTATCA13000.014.6833191
GGCGAGG7250.012.63785319
TAGGACC6200.012.4915914
AATACCG803.0432193E-511.8060775
GTGGTAT3550.011.5422311
TGGTATC3450.011.2974322
GTATTAT2950.011.1795961
CCAACGA951.4496189E-510.93726319
GTCCTAC19450.010.8930751
GTATCAA28600.010.7276981
ACTGACC1502.3483153E-910.7041778
TGGCGAG17300.010.7016218
TCCAACG1606.020855E-1010.62654518
GACTTAC959.848784E-510.5198761
TATACTG3600.010.494295
TCCTACA20900.010.4720572
TGTAGGA20100.010.2922072
CCTACAG22950.010.2885213
GTCCTAG2250.010.2159681
Produced by FastQC (version 0.11.5)