FastQCFastQC Report
Thu 26 May 2016
SRR1511818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975961
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78810.39884390430782796No Hit
GGTATCAACGCAGAGTACTTTTTTT52430.26533924505594997No Hit
TATCAACGCAGAGTACTTTTTTTTT48290.24438741452893048No Hit
GTCCTACAGTGGACATTTCTAAATT39420.1994978645833597No Hit
CTGTAGGACGTGGAATATGGCAAGA36750.185985452142021No Hit
GTCCTAAAGTGTGTATTTCTCATTT31050.15713872895264633No Hit
ATTCCATTCCATTCCATTCCATTCC29110.14732072141099953No Hit
CTTTAGGACGTGAAATATGGCGAGG27880.14109590219645024No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25500.1290511300577289No Hit
GAATGGAATGGAATGGAATGGAATG23760.12024528824202503No Hit
GTCCTACAGTGTGCATTTCTCATTT20930.10592314321993196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1500.015.19613318
GGTATCA16050.013.6071961
CCAACGA1351.8189894E-1213.36696919
TAGGACC7600.012.7509524
TTACACG604.0903236E-412.6676124
GGCGAGG9100.012.1068119
TTAGGAC11450.011.5336123
GTGGTAT4950.011.3672391
TGGCGAG20650.011.03835418
TGTAGGA22300.011.0344552
AGGACCT13750.010.9171785
ACACCGT700.001495512810.8538326
CCCGTTT1408.392817E-910.85383212
ATCCCGT1408.392817E-910.85383210
CCGTTTC1408.392817E-910.85383213
TAGGACT2550.010.8047284
AGGACGT19250.010.7592445
TAGGACA6850.010.6796284
GGACGTG19200.010.6347766
GTAGGAC21650.010.619733
Produced by FastQC (version 0.11.5)