FastQCFastQC Report
Thu 26 May 2016
SRR1511817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463797
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42190.2882230254604976No Hit
GGTATCAACGCAGAGTACTTTTTTT27790.1898487290245847No Hit
TATCAACGCAGAGTACTTTTTTTTT27210.18588643097369376No Hit
GTCCTACAGTGGACATTTCTAAATT25790.17618563229737455No Hit
CTGTAGGACGTGGAATATGGCAAGA25420.1736579594028407No Hit
GTCCTAAAGTGTGTATTTCTCATTT25010.17085702457376262No Hit
CTTTAGGACGTGAAATATGGCGAGG23120.15794539816654904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG902.4811015E-914.6928118
GGTATCA9500.013.5678581
TAGGACC4900.012.3368214
CGGTTTC951.1145821E-611.93100513
GTAATAC855.643988E-511.112213
CGGTAGG1001.372153E-510.9910171
GCCGGTT951.4496554E-510.93675411
TTAGGAC10350.010.7686433
GTACTAA1301.9169602E-710.7604351
AGGACGT17150.010.5744185
GGCGAGG8350.010.52000219
TCGAACT901.00858226E-410.49486419
GTTCTAG1052.3536477E-510.4676341
GAGTTAG1052.3536477E-510.4676341
GGACGTG17050.010.41484456
AGGCGTG1106.476217E-610.3036977
GTCCTAT1751.0732037E-910.2773141
TCTTAAG1751.0732037E-910.2773142
GTCCTAC14200.010.2029291
GACGTGA9200.010.0610377
Produced by FastQC (version 0.11.5)