FastQCFastQC Report
Thu 26 May 2016
SRR1511817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463797
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53160.36316511100924515No Hit
GGTATCAACGCAGAGTACTTTTTTT38160.2606918855551692No Hit
TATCAACGCAGAGTACTTTTTTTTT35610.24327143722797628No Hit
GTCCTACAGTGGACATTTCTAAATT28510.19476744384638034No Hit
CTGTAGGACGTGGAATATGGCAAGA28250.19299124127184303No Hit
GTCCTAAAGTGTGTATTTCTCATTT27890.19053188386094522No Hit
CTTTAGGACGTGAAATATGGCGAGG24530.16757788135923218No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18230.1245391266685203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACT500.001503381113.2954088
GGTATCA11450.013.2482271
TAGGACC5200.013.1555974
GGCGAGG7150.012.61845219
CTTGGAC1457.2759576E-1212.4499333
TGGACGG600.00586956211.0848085
GTCCTAG1407.8107405E-910.9033031
CGCCCTA1053.4795194E-610.85339516
TGGCGAG15200.010.621695518
TAAGCGA909.5466436E-510.55191213
GCGACTA1002.4072657E-510.44639216
AGGACCT10650.010.3487965
GTATAAT2053.6379788E-1210.2384671
GGACCTG10150.010.1984496
TAGAAAT4600.010.1209134
AATGTCC4650.010.0072978
CGACTAA951.6494095E-49.99654917
TCAGACC1054.0977655E-59.9537063
GTCCTAA9500.09.942091
TGTAGGA16450.09.8766942
Produced by FastQC (version 0.11.5)