##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511817_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1463797 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.208507053915262 31.0 31.0 34.0 28.0 34.0 2 31.392592005585474 33.0 31.0 34.0 28.0 34.0 3 31.503179061031002 33.0 31.0 34.0 28.0 34.0 4 35.08175723819628 37.0 35.0 37.0 32.0 37.0 5 34.756231226051156 37.0 35.0 37.0 32.0 37.0 6 34.70712469010389 37.0 35.0 37.0 32.0 37.0 7 34.55501753316887 37.0 35.0 37.0 31.0 37.0 8 34.48131605680296 37.0 35.0 37.0 30.0 37.0 9 36.032333035250105 39.0 37.0 39.0 31.0 39.0 10 35.85372971798685 39.0 35.0 39.0 30.0 39.0 11 35.83900978072779 39.0 35.0 39.0 30.0 39.0 12 35.68384140697105 39.0 35.0 39.0 30.0 39.0 13 35.68004169977121 39.0 35.0 39.0 30.0 39.0 14 36.762093377701966 40.0 36.0 41.0 30.0 41.0 15 36.708256677667734 40.0 36.0 41.0 30.0 41.0 16 36.611457053129634 40.0 36.0 41.0 30.0 41.0 17 36.56555997860359 40.0 36.0 41.0 30.0 41.0 18 36.49985756221662 40.0 36.0 41.0 30.0 41.0 19 36.51513495382215 40.0 36.0 41.0 30.0 41.0 20 36.3932895066734 40.0 36.0 41.0 30.0 41.0 21 36.28658072123389 40.0 36.0 41.0 29.0 41.0 22 36.36411742885113 40.0 36.0 41.0 30.0 41.0 23 36.2739983754578 40.0 36.0 41.0 30.0 41.0 24 36.162993229252415 40.0 36.0 41.0 29.0 41.0 25 35.75112259418485 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.42103371041340765 1101 2 0.49472543808865055 1101 3 0.48490560160771423 1101 4 0.3265595086227293 1101 5 0.4676598843816677 1101 6 0.4661134591143963 1101 7 0.5383286992687601 1101 8 0.35452401485559193 1101 9 0.26097676894052313 1101 10 0.4197308606895902 1101 11 0.21685951597059017 1101 12 0.47724264186926746 1101 13 0.3016842131249291 1101 14 0.4514542928724836 1101 15 0.28064208570079074 1101 16 0.18043842196558302 1101 17 0.32908802312970664 1101 18 0.4554210140741475 1101 19 0.495379072356954 1101 20 0.37964104749809735 1101 21 0.17874224488011237 1101 22 0.1580466184672531 1101 23 0.059806036467378476 1101 24 0.12147441938997616 1101 25 0.10684628859067402 1102 1 0.4621066236280207 1102 2 0.42327385988179955 1102 3 0.5132685572062954 1102 4 0.5079349817359429 1102 5 0.5817901990928007 1102 6 0.5959171207635947 1102 7 0.5436077997286475 1102 8 0.5604909100119642 1102 9 0.4789268733015959 1102 10 0.5201692353313021 1102 11 0.6207263989175829 1102 12 0.766287927016279 1102 13 0.7820509594432181 1102 14 0.9097813761694837 1102 15 0.9186649811827436 1102 16 1.0032792524355258 1102 17 0.9982837742677972 1102 18 1.0701728440266436 1102 19 1.0519009126002103 1102 20 0.9763061443881966 1102 21 1.134484429427694 1102 22 0.7505739006026175 1102 23 0.9076749194260003 1102 24 0.7969359046311695 1102 25 1.0057590729673294 1103 1 0.4131818814698818 1103 2 0.4611670304464326 1103 3 0.4523437061727762 1103 4 0.40346791540588356 1103 5 0.39435782091415206 1103 6 0.4396491875132753 1103 7 0.6540357934048018 1103 8 0.2990062580389363 1103 9 0.5995143013701281 1103 10 0.526058713205785 1103 11 0.5790985758128642 1103 12 0.6246920860559939 1103 13 0.5959851941740126 1103 14 0.7721095855549933 1103 15 0.6036941620542464 1103 16 0.514383407619242 1103 17 0.6280416853120698 1103 18 0.8680354841186428 1103 19 0.9200290044910346 1103 20 0.7903776074692317 1103 21 0.6962224434953015 1103 22 0.829621966816994 1103 23 0.8707298720085035 1103 24 0.922642744046783 1103 25 1.0126363681398374 1104 1 0.23756426837421074 1104 2 0.283585464008727 1104 3 0.24758804669733436 1104 4 0.192574211758604 1104 5 0.4281623264152046 1104 6 0.17807687671628258 1104 7 0.3878875485983997 1104 8 0.28400668858520106 1104 9 0.44053895731950377 1104 10 0.5250320259612167 1104 11 0.4432844128279214 1104 12 0.3114729909980056 1104 13 0.44917002216526214 1104 14 0.4198487760838603 1104 15 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-0.675676774150169 2315 1 -0.3413005070932229 2315 2 -0.28896647663775 2315 3 -0.446934461266828 2315 4 -0.2836183084516435 2315 5 -0.3863532799763263 2315 6 -0.5054161606910839 2315 7 -0.46719247176965695 2315 8 -0.3641713383225209 2315 9 -0.5470790322187256 2315 10 -0.5221494811907519 2315 11 -0.5871094397225036 2315 12 -0.5017729785256577 2315 13 -0.7532131245310296 2315 14 -0.8686950341733919 2315 15 -1.0832246988556449 2315 16 -0.9454482073693242 2315 17 -0.9763250822833953 2315 18 -0.9264632574653007 2315 19 -0.7793040988779438 2315 20 -0.753908417085853 2315 21 -0.9721370163439786 2315 22 -0.9289167374616198 2315 23 -0.9337493226052231 2315 24 -0.9726212678091599 2315 25 -0.9105031563449728 2316 1 -1.4664537612676583 2316 2 -1.0255310717026305 2316 3 -0.879719031315279 2316 4 -0.7330869131577984 2316 5 -0.733022621150532 2316 6 -0.839689811736342 2316 7 -0.8133787707893845 2316 8 -0.8808574930166841 2316 9 -1.1673116817587612 2316 10 -1.133878501391436 2316 11 -1.0589946576867177 2316 12 -1.1376987902957794 2316 13 -1.2047870885262881 2316 14 -1.3112513415264928 2316 15 -1.2522245471914957 2316 16 -1.2539386272506974 2316 17 -1.389286332909215 2316 18 -1.2851290695941913 2316 19 -1.1933119951983713 2316 20 -1.1969151506478255 2316 21 -1.3680701340859116 2316 22 -1.344911271029133 2316 23 -1.207108455151534 2316 24 -1.185463144074042 2316 25 -1.3258499372326824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16963.0 3 2.0 4 319.0 5 2721.0 6 3542.0 7 2825.0 8 2479.0 9 2172.0 10 2028.0 11 1964.0 12 1941.0 13 1889.0 14 1886.0 15 1816.0 16 1970.0 17 2018.0 18 2159.0 19 2420.0 20 2988.0 21 4010.0 22 4992.0 23 6483.0 24 7986.0 25 9415.0 26 11221.0 27 12746.0 28 15119.0 29 18418.0 30 23973.0 31 31397.0 32 40183.0 33 52613.0 34 69296.0 35 93144.0 36 138163.0 37 248022.0 38 622514.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51112758880636 19.46163665584489 13.037439046055768 24.989796709292985 2 15.418599710205719 20.761622000864875 40.181801165052256 23.63797712387715 3 18.73921042330323 24.934536687805757 30.22516100251606 26.101091886374956 4 12.968738151533307 17.33259461523695 37.71144496128903 31.987222271940713 5 13.303790159184636 37.77565182332863 35.64902162437346 13.271536393113273 6 31.661835623382206 37.18432268955326 17.942788515074152 13.211053171990377 7 27.770858937407304 31.657463432429495 22.384524630122893 18.187153000040308 8 25.10621349818315 36.85060155199116 20.573549474414826 17.469635475410865 9 26.468150979951453 14.933218198971579 20.31210611853966 38.28652470253731 10 15.44783873720195 28.736839876021058 34.37102275793706 21.444298628839928 11 34.39910042171148 21.980643490866562 24.75596001358112 18.86429607384084 12 23.473610070248814 24.55456596782204 31.71881073673467 20.253013225194476 13 29.36281465257819 21.84865797648171 25.717500445758528 23.071026925181563 14 21.077991005583424 22.18893740047288 27.59207731673176 29.140994277211934 15 23.1171398766359 31.029712453297826 23.382203953143776 22.4709437169225 16 22.23873938804356 27.69140802993858 27.84832869585059 22.221523886167276 17 21.795576845696502 28.407081036509847 27.779535003829082 22.017807113964576 18 21.67117434999525 26.912884778422146 30.079990599789454 21.335950271793152 19 23.717223084894968 26.464735205769653 27.69741979249855 22.12062191683683 20 23.7959225220437 26.894781175258593 28.178019219878163 21.131277082819544 21 23.094869028970546 26.393618787304522 27.357003737540108 23.154508446184817 22 23.42087051688178 27.700767251196716 27.464463993299614 21.413898238621886 23 22.42517234288634 27.469655970055957 27.945609944548323 22.15956174250938 24 23.28071209805627 26.484716112455086 28.55859444119172 21.67597734829692 25 22.89484129288419 27.03667243477067 28.297366369790346 21.771119902554794 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 420.0 1 420.0 2 484.0 3 548.0 4 548.0 5 548.0 6 1269.0 7 1990.0 8 1990.0 9 1990.0 10 2635.0 11 3280.0 12 3280.0 13 3280.0 14 4758.0 15 6236.0 16 6236.0 17 6236.0 18 11566.5 19 16897.0 20 16897.0 21 16897.0 22 26715.0 23 36533.0 24 36533.0 25 36533.0 26 53304.5 27 70076.0 28 70076.0 29 70076.0 30 90586.5 31 111097.0 32 111097.0 33 111097.0 34 124304.0 35 137511.0 36 137511.0 37 137511.0 38 147251.0 39 156991.0 40 156991.0 41 156991.0 42 166085.0 43 175179.0 44 175179.0 45 175179.0 46 181625.0 47 188071.0 48 188071.0 49 188071.0 50 183646.5 51 179222.0 52 179222.0 53 179222.0 54 161031.5 55 142841.0 56 142841.0 57 142841.0 58 123316.5 59 103792.0 60 103792.0 61 103792.0 62 85970.0 63 68148.0 64 68148.0 65 68148.0 66 52754.0 67 37360.0 68 37360.0 69 37360.0 70 27332.5 71 17305.0 72 17305.0 73 17305.0 74 12098.5 75 6892.0 76 6892.0 77 6892.0 78 4650.0 79 2408.0 80 2408.0 81 2408.0 82 1569.5 83 731.0 84 731.0 85 731.0 86 472.5 87 214.0 88 214.0 89 214.0 90 132.5 91 51.0 92 51.0 93 51.0 94 27.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4053840798963244 2 0.0 3 0.0 4 0.0 5 0.02746282442169235 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0045088219199793415 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1463797.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.77423468526679 #Duplication Level Percentage of deduplicated Percentage of total 1 81.91435632028198 57.155115219839224 2 12.400440583881506 17.3046250300091 3 3.182979350993599 6.6626984470375685 4 1.0876734685954812 3.0356633543487708 5 0.4728349052256115 1.649584682229885 6 0.2654315975993394 1.111217195026896 7 0.14812216997565264 0.7234577734980516 8 0.08778307767804003 0.4899997650642056 9 0.07494092202653894 0.4706050932909008 >10 0.31659293131980837 4.027207240984065 >50 0.024748030969086086 1.176056530203032 >100 0.020276561814293586 2.766230008033693 >500 0.0021549167194640823 0.9601703792436059 >1k 0.0015672121596102418 2.10405048725131 >5k 9.795075997564011E-5 0.36331879393971167 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5316 0.36316511100924515 No Hit GGTATCAACGCAGAGTACTTTTTTT 3816 0.2606918855551692 No Hit TATCAACGCAGAGTACTTTTTTTTT 3561 0.24327143722797628 No Hit GTCCTACAGTGGACATTTCTAAATT 2851 0.19476744384638034 No Hit CTGTAGGACGTGGAATATGGCAAGA 2825 0.19299124127184303 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2789 0.19053188386094522 No Hit CTTTAGGACGTGAAATATGGCGAGG 2453 0.16757788135923218 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1823 0.1245391266685203 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 6.831548363605063E-5 0.0 11 0.0 0.0 0.0 6.831548363605063E-5 0.0 12 0.0 0.0 0.0 6.831548363605063E-5 0.0 13 0.0 0.0 0.0 6.831548363605063E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGACT 50 0.0015033811 13.295408 8 GGTATCA 1145 0.0 13.248227 1 TAGGACC 520 0.0 13.155597 4 GGCGAGG 715 0.0 12.618452 19 CTTGGAC 145 7.2759576E-12 12.449933 3 TGGACGG 60 0.005869562 11.084808 5 GTCCTAG 140 7.8107405E-9 10.903303 1 CGCCCTA 105 3.4795194E-6 10.853395 16 TGGCGAG 1520 0.0 10.6216955 18 TAAGCGA 90 9.5466436E-5 10.551912 13 GCGACTA 100 2.4072657E-5 10.446392 16 AGGACCT 1065 0.0 10.348796 5 GTATAAT 205 3.6379788E-12 10.238467 1 GGACCTG 1015 0.0 10.198449 6 TAGAAAT 460 0.0 10.120913 4 AATGTCC 465 0.0 10.007297 8 CGACTAA 95 1.6494095E-4 9.996549 17 TCAGACC 105 4.0977655E-5 9.953706 3 GTCCTAA 950 0.0 9.94209 1 TGTAGGA 1645 0.0 9.876694 2 >>END_MODULE