FastQCFastQC Report
Thu 26 May 2016
SRR1511816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915645
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46780.2441997342931493No Hit
GTCCTACAGTGGACATTTCTAAATT39170.20447421103596963No Hit
GTCCTAAAGTGTGTATTTCTCATTT35810.18693442678575622No Hit
CTGTAGGACGTGGAATATGGCAAGA35310.1843243398437602No Hit
GGTATCAACGCAGAGTACTTTTTTT30860.16109456605999548No Hit
CTTTAGGACGTGAAATATGGCGAGG30590.15968511911131758No Hit
TATCAACGCAGAGTACTTTTTTTTT28540.14898376264913382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8000.013.1051254
GGTATCA9850.012.7824121
GGCGAAA550.003203660512.021708519
ACCGTGC550.003204912912.0210818
GCCGGTT951.1155262E-611.930696511
TCCAACG1657.2759576E-1211.44924618
GTATAAA2750.011.2643851
TGTAGGA21250.011.2387352
GGCGAGG9150.011.14893819
GTAGGAC21300.011.0858393
TGATGCG600.006135319411.0193235
AGGACCT15350.011.0141965
CCAACGA1053.7137015E-610.79500319
TGCACCG700.001565499110.7944395
GGACCTG14650.010.7668096
CTGTAGG21750.010.7506591
GTCCTAC21000.010.6587721
TACTAGA1501.1135853E-810.6587722
TAGGACA6650.010.6524084
TGGCGAG20700.010.49514318
Produced by FastQC (version 0.11.5)