FastQCFastQC Report
Thu 26 May 2016
SRR1511816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915645
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57980.30266568179386055No Hit
GTCCTACAGTGGACATTTCTAAATT41910.2187774874781079No Hit
CTGTAGGACGTGGAATATGGCAAGA40210.20990319187532136No Hit
GTCCTAAAGTGTGTATTTCTCATTT38510.20102889627253484No Hit
GGTATCAACGCAGAGTACTTTTTTT37740.19700936238186093No Hit
TATCAACGCAGAGTACTTTTTTTTT37500.19575652064970284No Hit
CTTTAGGACGTGAAATATGGCGAGG35670.18620360244199735No Hit
GTCCTACAGTGTGCATTTCTCATTT21290.11113750199019129No Hit
CTGAAGGACCTGGAATATGGCGAGA19230.10038394378916761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11950.013.3279811
TCCAACG2200.012.51944218
TAGGACC7150.012.4888414
GCGATAA701.0938322E-412.21108111
CCAACGA1052.7302303E-711.75881919
AGGCCGT907.484816E-611.6080656
TGCGATA907.484816E-611.60806510
GGCGAGG10050.011.52931319
TAGGAGT1850.011.2966924
TGTCGAG1104.9867776E-711.22432718
GCGTGCG700.001494945710.8542949
CCAACGT1355.7103534E-810.55278619
GTCCTAC20800.010.270711
TAATCTG2900.010.1546285
TGGCGAG22150.010.1192418
ATAAGCG856.610363E-410.05618414
GCGTCGG856.610363E-410.05618418
GTCCTAA12350.010.039041
GCCCTAT951.5808696E-410.039041
CGTGCGC1054.117418E-59.94976910
Produced by FastQC (version 0.11.5)