FastQCFastQC Report
Thu 26 May 2016
SRR1511815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335699
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT52360.2241727208856963No Hit
GTATCAACGCAGAGTACTTTTTTTT49810.21325521824515914No Hit
CTGTAGGACGTGGAATATGGCAAGA46290.19818478322763336No Hit
GTCCTAAAGTGTGTATTTCTCATTT45230.19364652722803752No Hit
CTTTAGGACGTGAAATATGGCGAGG39230.16795828572089125No Hit
GGTATCAACGCAGAGTACTTTTTTT31920.1366614448180181No Hit
TATCAACGCAGAGTACTTTTTTTTT30360.12998250202616005No Hit
GTCCTACAGTGTGCATTTCTCATTT27780.11893655817808717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT454.6199438E-415.538811
GGTATCA11250.013.7629451
CCAACGA1257.2759576E-1213.60229719
TAGGACC8350.013.1217994
TCCAACG1900.011.93183918
AGGGTCG658.423338E-411.6251485
CGGTAGG709.805029E-411.4162681
GACAGTC1605.0931703E-1111.2164527
GGACCGT600.006134558511.0196726
TATACCG600.006134558511.0196725
GTCCTAA14700.010.8046831
TACACGA700.001565310510.794785
GGCGAGG11950.010.59221119
CTCGAAC1252.424349E-710.57956418
TAGGACT4550.010.3795974
TGTAGGA28450.010.217462
AGGACCT18250.010.1958925
TTAGGAC17250.010.1846413
GTATCAA23050.010.1409151
GTAGGAC30100.010.0102763
Produced by FastQC (version 0.11.5)