FastQCFastQC Report
Thu 26 May 2016
SRR1511815_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511815_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335699
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60140.25748180737329596No Hit
GTATCAACGCAGAGTACTTTTTTTT57010.24408110805373465No Hit
CTGTAGGACGTGGAATATGGCAAGA52600.22520025054598214No Hit
GTCCTAAAGTGTGTATTTCTCATTT50190.21488214020727844No Hit
CTTTAGGACGTGAAATATGGCGAGG45570.1951021942467758No Hit
TATCAACGCAGAGTACTTTTTTTTT37150.15905302866508056No Hit
GGTATCAACGCAGAGTACTTTTTTT36080.1544719589296395No Hit
GTCCTACAGTGTGCATTTCTCATTT31770.13601923878033942No Hit
CTGAAGGACCTGGAATATGGCGAGA26160.11200073297115767No Hit
CTGTAGGACCTGGAATATGGCGAGA24920.10669182972634746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14300.013.3373091
TAGGACC8900.012.2753214
GCGTGCG701.09415836E-412.2109279
AGAACCG550.003069161412.090955
AAGGCGT2000.010.9219966
TATTCCG700.001491846910.8571795
CTTACAC3400.010.6176823
TGTAGGA31700.010.4290572
AATGTCC7650.010.4285058
GAAATGT7250.010.3488796
CCAACGA1856.002665E-1110.26766719
AAATGTC7500.010.2571797
GGCGAGG12050.010.16754619
TCCAACG2450.010.07907718
CTTAGAC1701.5606929E-910.0588573
TGCACCG856.595383E-410.0588575
GTCTTAC1909.276846E-1110.038081
GTAGGAC31950.010.0203783
CTGTAGG32200.09.9804071
GTCCTAC28400.09.8383791
Produced by FastQC (version 0.11.5)