FastQCFastQC Report
Thu 26 May 2016
SRR1511814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990335
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55680.2797519010618815No Hit
GGTATCAACGCAGAGTACTTTTTTT35960.18067310276913182No Hit
TATCAACGCAGAGTACTTTTTTTTT35870.1802209175842258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11300.013.0878071
TAGGACC2750.012.0211744
GGACCGT1052.9331568E-711.6940696
GTACTAG802.3615255E-411.2418081
AACCGTG600.006135099611.0194117
ATACCGC600.006135099611.0194116
GAACACG650.007316318310.7613881
GTATCAA22950.010.4934431
GTATAAT3500.010.2782241
GGCGAGG4000.010.15359919
AGCGTAC1103.9269777E-59.9927181
TGGACCG951.7431445E-49.9423255
GTCCTAA6150.09.9114761
CTACACT2650.09.6234224
GAATAGG1904.440153E-99.46678451
GTCCTAG2750.09.447661
GACGTGA5100.09.44520957
ACATCGT800.00473703029.44520958
TCGGTTT800.00473703029.445209513
CTAATAC1701.8295395E-89.44520953
Produced by FastQC (version 0.11.5)