FastQCFastQC Report
Thu 26 May 2016
SRR1511814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990335
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69930.3513478886720075No Hit
GGTATCAACGCAGAGTACTTTTTTT46470.23347828380649488No Hit
TATCAACGCAGAGTACTTTTTTTTT46240.2323226994450683No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22680.11395066659632676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.013.8976931
CGAACCG951.3652891E-510.99659712
CAAGTCG951.3652891E-510.9965976
GTGCGCC1605.475158E-1010.68419311
TCGAACT1355.7043508E-810.55361619
GACCGTG909.547136E-510.552297
GGACCGT909.547136E-510.552296
TAGGACC3000.010.450184
AAGTCGT1002.4075085E-510.4467667
TGGCGAG8300.010.29931218
GGCGAGG4450.010.24530719
TCTGCGG750.002654935210.130198510
CGAACGG951.6494907E-49.99690614
GCGAACG1054.1197643E-59.94930213
TGTGCGG1054.1197643E-59.94930210
GTCCTAA4800.09.9357031
GCGAACC1252.5805384E-69.87694411
CCCGTGC1252.5805384E-69.8769448
GTCTTAA2350.09.7412181
AAGGCGT2251.8189894E-129.7081076
Produced by FastQC (version 0.11.5)