##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511814_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1990335 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.462335737451234 33.0 31.0 34.0 28.0 34.0 2 31.639619963473486 33.0 31.0 34.0 28.0 34.0 3 31.7562762047595 34.0 31.0 34.0 28.0 34.0 4 35.32106303712692 37.0 35.0 37.0 33.0 37.0 5 35.0365908251626 37.0 35.0 37.0 32.0 37.0 6 34.99902277757262 37.0 35.0 37.0 32.0 37.0 7 34.86430826971339 37.0 35.0 37.0 32.0 37.0 8 34.795917772636265 37.0 35.0 37.0 32.0 37.0 9 36.38915257984209 39.0 37.0 39.0 32.0 39.0 10 36.235612095451266 39.0 37.0 39.0 32.0 39.0 11 36.22833593339815 39.0 37.0 39.0 32.0 39.0 12 36.08083312608179 39.0 37.0 39.0 31.0 39.0 13 36.075572705097386 39.0 37.0 39.0 32.0 39.0 14 37.22113413068654 40.0 37.0 41.0 31.0 41.0 15 37.1608568406826 40.0 37.0 41.0 31.0 41.0 16 37.07458442925437 40.0 37.0 41.0 31.0 41.0 17 37.02760841767843 40.0 37.0 41.0 31.0 41.0 18 36.96226966817144 40.0 37.0 41.0 31.0 41.0 19 36.97970542647343 40.0 37.0 41.0 31.0 41.0 20 36.86482778024805 40.0 37.0 41.0 31.0 41.0 21 36.77347783162131 40.0 37.0 41.0 30.0 41.0 22 36.85002926643002 40.0 37.0 41.0 31.0 41.0 23 36.772759359605296 40.0 37.0 41.0 31.0 41.0 24 36.66452280646223 40.0 37.0 41.0 30.0 41.0 25 36.25893229029284 40.0 36.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36363396149144833 1101 2 0.2771948614161559 1101 3 0.36585892820257016 1101 4 0.34795102747216333 1101 5 0.5743411611570153 1101 6 0.4875495274624413 1101 7 0.4633918812432398 1101 8 0.39773740439014205 1101 9 0.44548380892883443 1101 10 0.47331779937076135 1101 11 0.32953384908922345 1101 12 0.5552978660689405 1101 13 0.5137022317909654 1101 14 0.5262730709706318 1101 15 0.46454334835642186 1101 16 0.6218170475369575 1101 17 0.5701010687753438 1101 18 0.5704791913873777 1101 19 0.4547930407782914 1101 20 0.6320838361772232 1101 21 0.5773553909654225 1101 22 0.4896773438584603 1101 23 0.4010614251622684 1101 24 0.26071284785296456 1101 25 0.37184300743850685 1102 1 0.5243614433786767 1102 2 0.5100801053195205 1102 3 0.47521166816341065 1102 4 0.5276997723392611 1102 5 0.4677189859996318 1102 6 0.5678075629794677 1102 7 0.6681067876764786 1102 8 0.6047054553217492 1102 9 0.6232016128970059 1102 10 0.6946605185841364 1102 11 0.7438349997229494 1102 12 0.7961183179421809 1102 13 0.8414258765394749 1102 14 0.9198229448859649 1102 15 0.9181364440461763 1102 16 0.9325871925152853 1102 17 1.0169302868980168 1102 18 0.9800893066031193 1102 19 1.0348891851014344 1102 20 1.0369159391374865 1102 21 1.110826236583442 1102 22 0.9051695057852029 1102 23 0.9905358311168158 1102 24 0.9851067399204254 1102 25 1.1004968599410248 1103 1 0.31138989742728285 1103 2 0.31836381524692214 1103 3 0.2958184209016608 1103 4 0.2573121535953362 1103 5 0.3744133873918045 1103 6 0.36488614717364243 1103 7 0.3068743474408535 1103 8 0.12169050279459981 1103 9 0.5164902789852519 1103 10 0.4957571773160012 1103 11 0.2925444203835923 1103 12 0.5199310022532728 1103 13 0.42700802150655903 1103 14 0.5524045732258074 1103 15 0.58932174513604 1103 16 0.2842490513351521 1103 17 0.47929930706131074 1103 18 0.5549631778714783 1103 19 0.596076142364744 1103 20 0.6829780777765038 1103 21 0.3895015202958376 1103 22 0.38355149848321446 1103 23 0.5747225881561704 1103 24 0.5876034831912946 1103 25 0.5583268525006062 1104 1 0.23483669008581032 1104 2 0.28742433918549537 1104 3 0.29640577196620654 1104 4 0.21391720493350874 1104 5 0.2601340320378682 1104 6 0.1824696051777437 1104 7 0.2155695235450068 1104 8 0.3174929362251717 1104 9 0.34703414107736563 1104 10 0.3442744580531496 1104 11 0.32360707949503364 1104 12 0.572409659450912 1104 13 0.5626777861971775 1104 14 0.5899373178988867 1104 15 0.6840227316715968 1104 16 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-0.7375365482718266 2314 19 -0.7731506751938184 2314 20 -0.7780398381173796 2314 21 -0.6053348644935923 2314 22 -0.596592946758669 2314 23 -0.7003674068199786 2314 24 -0.7819765150711291 2314 25 -0.6966658383586264 2315 1 -0.44905821593272677 2315 2 -0.44396844468542085 2315 3 -0.5407662061615284 2315 4 -0.565553596170794 2315 5 -0.4377888454176926 2315 6 -0.529011766759119 2315 7 -0.6020988169187831 2315 8 -0.5750487069397039 2315 9 -0.6236912880078052 2315 10 -0.6061194890028148 2315 11 -0.5926006734474143 2315 12 -0.5395686426655573 2315 13 -0.6730745383285424 2315 14 -0.7518307278451601 2315 15 -0.8318553143116603 2315 16 -0.8902123934989064 2315 17 -0.9392536609832334 2315 18 -0.8610608180430148 2315 19 -0.8162382018986918 2315 20 -0.9059773740816013 2315 21 -1.0377269257230495 2315 22 -0.96135791195276 2315 23 -0.9129687008577392 2315 24 -0.8194412071677704 2315 25 -0.7209666514071387 2316 1 -1.6575820312069354 2316 2 -1.181878437809587 2316 3 -0.9639996222184486 2316 4 -0.8417735636892587 2316 5 -0.8549744855232717 2316 6 -0.8997399396850909 2316 7 -0.9823336303224721 2316 8 -0.911918945918039 2316 9 -1.3003040898729097 2316 10 -1.3031404541332208 2316 11 -1.088934794585306 2316 12 -1.2772703892257908 2316 13 -1.2074672593101852 2316 14 -1.5574639822337417 2316 15 -1.4384122469506693 2316 16 -1.3951536285365762 2316 17 -1.5277424316877486 2316 18 -1.5063245025531629 2316 19 -1.419206886051498 2316 20 -1.3120372258691901 2316 21 -1.67794463579947 2316 22 -1.5997063318665496 2316 23 -1.5732298346555424 2316 24 -1.3470505119692646 2316 25 -1.3479699463853123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16961.0 3 0.0 4 287.0 5 2764.0 6 3907.0 7 3129.0 8 2586.0 9 2448.0 10 2238.0 11 2247.0 12 2161.0 13 2139.0 14 2109.0 15 2129.0 16 2261.0 17 2506.0 18 2607.0 19 2806.0 20 3503.0 21 4511.0 22 5929.0 23 7724.0 24 9543.0 25 11304.0 26 13749.0 27 15525.0 28 18572.0 29 23184.0 30 29783.0 31 39222.0 32 51520.0 33 67315.0 34 90096.0 35 121260.0 36 180342.0 37 325418.0 38 916550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.218215414922035 19.01373200241801 12.930052265775352 24.8380003168846 2 15.630685286647724 20.639389851457164 39.55977260109479 24.170152260800318 3 18.669319486418114 24.948513692418615 29.963096664631834 26.419070156531436 4 12.770413020923613 17.11631459025742 37.97184896009968 32.141423428719285 5 13.44623466227606 37.65533733192207 35.5221033099429 13.376324695858976 6 31.77470124376047 37.19283437210319 17.89146048278305 13.14100390135329 7 27.8319981309679 31.68491736315746 22.30237623314668 18.18070827272796 8 25.589410827825464 35.74257599851281 20.634466057221523 18.033547116440197 9 26.54100942806111 14.94291162040561 19.924083131734104 38.59199581979918 10 15.071432698515578 28.874134253781396 34.29005669899791 21.764376348705117 11 34.56473407742918 22.423210163113243 24.058512762926846 18.953542996530732 12 22.92568838914052 24.85878005461392 31.48796559373171 20.727565962513847 13 28.9922048298402 21.539288612218545 25.78591041206631 23.68259614587494 14 21.52220606078876 21.534716517571162 27.859782398440462 29.08329502319961 15 23.585225602725167 29.499255150514863 23.721785528566798 23.19373371819317 16 22.952618529041594 27.84566417211173 26.562965530928214 22.638751767918468 17 22.19530882992059 27.829285019858467 27.337608995470614 22.63779715475033 18 22.034693674529 27.14077134792452 28.717089694493485 22.107445283052996 19 23.453438742724213 26.821615456694474 27.762763554878955 21.96218224570236 20 23.714902265196564 26.550354588549162 27.959614838708056 21.77512830754622 21 23.662247812554167 26.313761251246646 27.413576106534833 22.610414829664354 22 23.45926690732967 26.803377320903266 27.523557592063646 22.213798179703417 23 23.123695257331054 26.698671329198355 27.378707604498743 22.798925808971855 24 23.07217298766082 26.498282121908552 27.924939932048602 22.50460495838203 25 23.26364482727262 26.590726275452397 27.702550276762732 22.443078620512246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 296.0 1 296.0 2 459.0 3 622.0 4 622.0 5 622.0 6 1547.0 7 2472.0 8 2472.0 9 2472.0 10 3342.0 11 4212.0 12 4212.0 13 4212.0 14 6308.0 15 8404.0 16 8404.0 17 8404.0 18 15674.5 19 22945.0 20 22945.0 21 22945.0 22 35696.0 23 48447.0 24 48447.0 25 48447.0 26 68622.0 27 88797.0 28 88797.0 29 88797.0 30 112554.5 31 136312.0 32 136312.0 33 136312.0 34 155426.5 35 174541.0 36 174541.0 37 174541.0 38 191116.0 39 207691.0 40 207691.0 41 207691.0 42 222777.0 43 237863.0 44 237863.0 45 237863.0 46 249051.0 47 260239.0 48 260239.0 49 260239.0 50 256680.5 51 253122.0 52 253122.0 53 253122.0 54 229919.5 55 206717.0 56 206717.0 57 206717.0 58 178355.5 59 149994.0 60 149994.0 61 149994.0 62 123804.0 63 97614.0 64 97614.0 65 97614.0 66 74618.5 67 51623.0 68 51623.0 69 51623.0 70 37935.0 71 24247.0 72 24247.0 73 24247.0 74 16900.5 75 9554.0 76 9554.0 77 9554.0 78 6404.0 79 3254.0 80 3254.0 81 3254.0 82 2127.5 83 1001.0 84 1001.0 85 1001.0 86 640.5 87 280.0 88 280.0 89 280.0 90 176.0 91 72.0 92 72.0 93 72.0 94 42.5 95 13.0 96 13.0 97 13.0 98 8.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42912374047584956 2 0.0 3 0.0 4 0.0 5 0.03180369133839279 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 5.024279832289539E-5 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00447160905073769 25 1.0048559664579078E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1990335.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.32724060278268 #Duplication Level Percentage of deduplicated Percentage of total 1 77.20994240680659 51.21122426943254 2 14.628162111644668 19.404912559111313 3 4.402551254019888 8.76027228874379 4 1.707381504715123 4.529836154559243 5 0.7686756195314121 2.5492066381076506 6 0.40367231461888325 1.6064682441845317 7 0.23216976048411664 1.0779425695024303 8 0.15137711028600742 0.8032340812555187 9 0.08897143922895549 0.5311107050863308 >10 0.36710800748941547 4.150454562974569 >50 0.02204391081106441 1.0245418229008254 >100 0.015522846992720996 2.1402871508259564 >500 0.0014378923140788162 0.6363282165670309 >1k 9.081425141550263E-4 1.2231636807845077 >5k 7.567854284625219E-5 0.3510170559638075 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6993 0.3513478886720075 No Hit GGTATCAACGCAGAGTACTTTTTTT 4647 0.23347828380649488 No Hit TATCAACGCAGAGTACTTTTTTTTT 4624 0.2323226994450683 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2268 0.11395066659632676 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.024279832289539E-5 0.0 4 0.0 0.0 0.0 5.024279832289539E-5 0.0 5 0.0 0.0 0.0 5.024279832289539E-5 0.0 6 0.0 0.0 0.0 5.024279832289539E-5 0.0 7 0.0 0.0 0.0 5.024279832289539E-5 0.0 8 0.0 0.0 0.0 5.024279832289539E-5 0.0 9 0.0 0.0 0.0 5.024279832289539E-5 0.0 10 0.0 0.0 0.0 5.024279832289539E-5 0.0 11 0.0 0.0 0.0 5.024279832289539E-5 0.0 12 0.0 0.0 0.0 5.024279832289539E-5 0.0 13 0.0 0.0 0.0 5.024279832289539E-5 5.024279832289539E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1455 0.0 13.897693 1 CGAACCG 95 1.3652891E-5 10.996597 12 CAAGTCG 95 1.3652891E-5 10.996597 6 GTGCGCC 160 5.475158E-10 10.684193 11 TCGAACT 135 5.7043508E-8 10.553616 19 GACCGTG 90 9.547136E-5 10.55229 7 GGACCGT 90 9.547136E-5 10.55229 6 TAGGACC 300 0.0 10.45018 4 AAGTCGT 100 2.4075085E-5 10.446766 7 TGGCGAG 830 0.0 10.299312 18 GGCGAGG 445 0.0 10.245307 19 TCTGCGG 75 0.0026549352 10.1301985 10 CGAACGG 95 1.6494907E-4 9.996906 14 GCGAACG 105 4.1197643E-5 9.949302 13 TGTGCGG 105 4.1197643E-5 9.949302 10 GTCCTAA 480 0.0 9.935703 1 GCGAACC 125 2.5805384E-6 9.876944 11 CCCGTGC 125 2.5805384E-6 9.876944 8 GTCTTAA 235 0.0 9.741218 1 AAGGCGT 225 1.8189894E-12 9.708107 6 >>END_MODULE