FastQCFastQC Report
Thu 26 May 2016
SRR1511813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268526
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58030.2558048706516919No Hit
CTGTAGGACGTGGAATATGGCAAGA54710.2411698168766856No Hit
GTATCAACGCAGAGTACTTTTTTTT54010.2380841127674975No Hit
GTCCTAAAGTGTGTATTTCTCATTT51660.22772496325808037No Hit
CTTTAGGACGTGAAATATGGCGAGG45020.19845485570806773No Hit
GGTATCAACGCAGAGTACTTTTTTT33230.1464827822118856No Hit
TATCAACGCAGAGTACTTTTTTTTT32850.1448076856954692No Hit
GTCCTACAGTGTGCATTTCTCATTT32010.14110484076444352No Hit
CTGAAGGACCTGGAATATGGCGAGA24890.10971882182527332No Hit
CTGTAGGACCTGGAATATGGCGAGA23720.1045612878142018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAC250.00450979419.9807421
GGACCGT400.00548597914.16805656
GGTATCA11100.012.6004691
TAGACCG604.297322E-412.5938275
TAGGACC10500.012.5938264
CGGTAGG904.2581505E-612.2104541
AAGGCGT1551.8189894E-1212.1875756
GGCGAGG13500.011.82524619
GTCCTAT2900.011.0238591
ATACCGT600.00613477111.0195996
CGGCCAT600.00613577711.01935612
GTATCAA24550.010.9059871
GATATAC5350.010.8306831
TAGAACG1053.7158989E-610.7947094
GTCCTAA16650.010.74041
GGCGTGC1855.456968E-1210.7215368
GTTCTAG2900.010.6793631
CCAACGA1606.020855E-1010.62697919
TGTAGGA32050.010.5670392
AGGACCT23600.010.5259855
Produced by FastQC (version 0.11.5)