##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511813_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2268526 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.802757826006843 31.0 28.0 34.0 18.0 34.0 2 26.133137111939647 31.0 25.0 34.0 2.0 34.0 3 27.856102156201867 31.0 27.0 34.0 16.0 34.0 4 32.14277023935366 35.0 32.0 37.0 20.0 37.0 5 32.493528396853286 35.0 33.0 37.0 25.0 37.0 6 32.68097434192952 36.0 33.0 37.0 25.0 37.0 7 32.64232766122143 37.0 33.0 37.0 25.0 37.0 8 32.65809560921938 37.0 35.0 37.0 25.0 37.0 9 34.14368669347409 39.0 35.0 39.0 25.0 39.0 10 34.00362041254982 39.0 35.0 39.0 25.0 39.0 11 33.987587093998485 39.0 35.0 39.0 24.0 39.0 12 33.85913187682222 38.0 34.0 39.0 23.0 39.0 13 33.849832005452 38.0 34.0 39.0 23.0 39.0 14 34.871189045221435 39.0 35.0 41.0 22.0 41.0 15 34.810485310725994 39.0 35.0 41.0 22.0 41.0 16 34.75744866931215 39.0 34.0 41.0 21.0 41.0 17 34.709804957051404 39.0 34.0 41.0 19.0 41.0 18 34.670200826439725 39.0 34.0 41.0 19.0 41.0 19 34.69173683704749 39.0 34.0 41.0 19.0 41.0 20 34.62021903209397 39.0 34.0 41.0 18.0 41.0 21 34.496200616611844 39.0 34.0 41.0 18.0 41.0 22 34.38371964879398 39.0 34.0 41.0 18.0 41.0 23 34.26595860043041 39.0 34.0 41.0 15.0 41.0 24 34.13861952651193 39.0 34.0 41.0 10.0 41.0 25 34.02539358155913 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7735510300506654 1101 2 3.3507416393771052 1101 3 1.9414746658147983 1101 4 1.1910479825412281 1101 5 0.34874193460818503 1101 6 0.3375557837944356 1101 7 0.41671527301549816 1101 8 0.3254580542615244 1101 9 0.31713268848115206 1101 10 0.2980688750995597 1101 11 0.15852226602336117 1101 12 0.2143661245291426 1101 13 0.2450365429859147 1101 14 0.1239491777309425 1101 15 0.3419933292406583 1101 16 0.2680756039688674 1101 17 0.5172661757515087 1101 18 0.5374709434613365 1101 19 0.692961266535626 1101 20 0.3703174578692199 1101 21 0.5826286807127872 1101 22 0.4393972589343349 1101 23 0.2755616414030726 1101 24 0.4312700354003596 1101 25 0.6020683701505902 1102 1 1.5115489295081836 1102 2 4.320911044584065 1102 3 2.485420029324306 1102 4 1.6696890472140566 1102 5 1.328547065453698 1102 6 1.3994718852174302 1102 7 1.2395454275527413 1102 8 1.242442787085949 1102 9 1.2363755977511488 1102 10 1.2748617048996493 1102 11 1.2922882066410608 1102 12 1.3867691749794702 1102 13 1.4243279177032946 1102 14 1.417027088082925 1102 15 1.4219053527010672 1102 16 1.4737384874204693 1102 17 1.3824215151588817 1102 18 1.3642270230954026 1102 19 1.5729924948493021 1102 20 1.7079404869783374 1102 21 1.6562022332785844 1102 22 1.6503175614423 1102 23 1.7005269432767705 1102 24 1.5354478950109964 1102 25 1.5586332183369578 1103 1 1.5205406223685323 1103 2 4.045419576241002 1103 3 2.652840774721703 1103 4 1.8453284724498005 1103 5 1.3546436078874677 1103 6 1.2116669907405537 1103 7 1.2161082923933364 1103 8 1.108516877790933 1103 9 1.1028542686088016 1103 10 1.4659301826724374 1103 11 1.461284614364459 1103 12 1.3271490582304821 1103 13 1.4086956770207593 1103 14 1.6627163561565652 1103 15 1.3789641762304825 1103 16 1.4704048547307522 1103 17 1.5295191801790864 1103 18 1.577674037015477 1103 19 1.4523409456800138 1103 20 1.547474838123371 1103 21 1.607773180741539 1103 22 1.724470457715313 1103 23 1.7442247430073508 1103 24 1.5960529691016987 1103 25 1.648090335534981 1104 1 1.564782660931531 1104 2 4.010580599873755 1104 3 2.343368418789037 1104 4 1.6640401746063915 1104 5 1.4317800469291058 1104 6 1.2741236005689558 1104 7 1.0270553974489474 1104 8 1.1654950399295814 1104 9 1.3292526804007068 1104 10 1.3808507450721592 1104 11 1.2513626650021905 1104 12 1.4372671404148107 1104 13 1.433580069612752 1104 14 1.5286378703933252 1104 15 1.4058397031402734 1104 16 1.4578501784913271 1104 17 1.4000380129549157 1104 18 1.375187512308507 1104 19 1.460476922400801 1104 20 1.3634748381233734 1104 21 1.4031519302996145 1104 22 1.2470385684547551 1104 23 1.5701440816165828 1104 24 1.4075228774533883 1104 25 1.4553675920686615 1105 1 1.4455938181343093 1105 2 4.217737410891946 1105 3 2.5329125957052234 1105 4 1.7186426892526967 1105 5 1.341854113046253 1105 6 1.203784905788126 1105 7 1.0636505676177777 1105 8 1.1286471203207498 1105 9 1.2796314359677794 1105 10 1.1488233891577693 1105 11 1.1244870798609057 1105 12 0.812618172527678 1105 13 1.0798831351475187 1105 14 1.2136476170436055 1105 15 1.3532265372113201 1105 16 1.1816276873717868 1105 17 1.154327434232421 1105 18 1.3246529998990368 1105 19 1.3535742298820779 1105 20 1.3256616262782686 1105 21 1.6526634393506754 1105 22 1.4950259983826086 1105 23 1.5615421756751786 1105 24 1.5855585298681447 1105 25 1.5754353710434899 1106 1 1.0875500471329147 1106 2 3.818183838952617 1106 3 2.1123835406980085 1106 4 1.155209289065752 1106 5 0.8623839689238935 1106 6 0.5879054756875206 1106 7 0.6867719258024962 1106 8 0.6127508719410812 1106 9 0.6193393928443385 1106 10 0.7281574997167866 1106 11 0.6871456951101678 1106 12 0.6950831144245342 1106 13 0.5100921966532255 1106 14 0.7667314830511174 1106 15 0.693753670129496 1106 16 0.9401618198975328 1106 17 0.7201279785954924 1106 18 0.7902239232683215 1106 19 0.6028055658826901 1106 20 0.7497129094443196 1106 21 0.8630248721765241 1106 22 0.7915329447895019 1106 23 0.7205641527039219 1106 24 0.8268539263504948 1106 25 0.820724365138787 1107 1 1.2469281812852628 1107 2 3.6057116434526755 1107 3 2.1863442498102437 1107 4 1.4743504946584167 1107 5 1.2024084202859413 1107 6 0.9943010279679427 1107 7 1.0206471574640332 1107 8 0.8677848989876438 1107 9 0.7992015111726474 1107 10 0.8162881301067344 1107 11 0.8556147610904219 1107 12 0.9440461370257864 1107 13 0.9747924469193094 1107 14 1.0821608745705404 1107 15 0.9994291191112055 1107 16 0.979576851641788 1107 17 1.0848586736949173 1107 18 0.9330124653700693 1107 19 1.1625484923473905 1107 20 1.1058922215547895 1107 21 0.9721194646602669 1107 22 1.0588494953421375 1107 23 1.209056846967087 1107 24 1.162081781713205 1107 25 1.266300758137703 1108 1 1.0961074603162295 1108 2 3.701990172145596 1108 3 2.1340684762325566 1108 4 1.4910399533915921 1108 5 1.1175489431636905 1108 6 0.8676115672673816 1108 7 0.9021238866362467 1108 8 0.9056405835965933 1108 9 0.797897139994646 1108 10 1.0060761038831671 1108 11 0.9286241342662933 1108 12 1.0228492397372406 1108 13 0.8714829050801711 1108 14 1.0797882174956257 1108 15 1.0719488120665588 1108 16 0.9595810185469986 1108 17 0.8921312414312297 1108 18 0.9261244805566449 1108 19 1.0079033865624965 1108 20 1.013192352247664 1108 21 0.8849197921892511 1108 22 0.7341619984776457 1108 23 0.8022496426673413 1108 24 0.8918609298348841 1108 25 0.7743828426290804 1109 1 0.3432360113302906 1109 2 2.3141539118277237 1109 3 1.0233555562775507 1109 4 0.5678254959590348 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-1.812342022582456 2314 15 -1.4541816038206719 2314 16 -1.461273561815446 2314 17 -1.3206771947792149 2314 18 -1.1416249733258965 2314 19 -1.1603640470921448 2314 20 -1.0996631580959075 2314 21 -1.1127899238953987 2314 22 -1.003738593769171 2314 23 -1.0279269305165002 2314 24 -1.109798248512 2314 25 -1.059489337544079 2315 1 -4.663349119462762 2315 2 -13.383179233572505 2315 3 -7.043894928660816 2315 4 -3.577699556663312 2315 5 -2.491125212766029 2315 6 -1.9355786345498025 2315 7 -1.6615270857895297 2315 8 -1.6760462706726607 2315 9 -1.6815554387115057 2315 10 -1.9215752483830215 2315 11 -2.030933158792191 2315 12 -1.9977157516272719 2315 13 -2.0211600405201082 2315 14 -1.9692018793587778 2315 15 -2.11955760329743 2315 16 -2.140224174315989 2315 17 -1.998302778540463 2315 18 -1.9899160786848071 2315 19 -2.162161985541367 2315 20 -2.0498384375441674 2315 21 -2.231107070569216 2315 22 -2.272460365460134 2315 23 -2.1536838028856238 2315 24 -2.3548998437647057 2315 25 -2.2802450404356414 2316 1 -1.9928190338227125 2316 2 -1.8079151896288153 2316 3 -1.4405213884083885 2316 4 -1.7973322850038649 2316 5 -1.9432455496020147 2316 6 -2.2130095246144457 2316 7 -2.1289356685700476 2316 8 -2.2392299213853555 2316 9 -2.248874265727302 2316 10 -2.5549431273821774 2316 11 -2.5646183312886883 2316 12 -2.2959257022673256 2316 13 -2.3453703987246044 2316 14 -2.7030102084418814 2316 15 -3.2596014966139997 2316 16 -2.943671785971187 2316 17 -2.72933371623062 2316 18 -2.7887954768174517 2316 19 -2.6959622148536084 2316 20 -2.9496910675082866 2316 21 -2.9697890002952576 2316 22 -2.9966166584096747 2316 23 -2.9270466331230125 2316 24 -2.7213353378192444 2316 25 -2.752416603313616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 115364.0 3 2.0 4 1058.0 5 5154.0 6 5941.0 7 5360.0 8 4643.0 9 4697.0 10 4442.0 11 4260.0 12 4248.0 13 4031.0 14 4191.0 15 4271.0 16 4637.0 17 4845.0 18 5406.0 19 6650.0 20 8700.0 21 11162.0 22 13776.0 23 16107.0 24 18657.0 25 21106.0 26 23354.0 27 26297.0 28 30125.0 29 35869.0 30 43507.0 31 54571.0 32 68895.0 33 89851.0 34 119443.0 35 167446.0 36 250439.0 37 336913.0 38 743108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.35512398799926 19.47806637437702 13.021010118464588 25.145799519159134 2 15.463806490507343 21.37443624307208 41.108831138918376 22.052926127502197 3 18.347243981334135 25.329354832168555 29.982728873286003 26.34067231321131 4 11.705927108615903 17.30595108894498 39.67192793911112 31.316193863327996 5 12.392688609068045 38.45634699351778 36.587988177287784 12.562976220126382 6 30.476292494471018 38.28109399114217 18.7458149676813 12.496798546705513 7 27.161072872869873 31.836267250188005 23.14904039010353 17.85361948683859 8 23.814053707120834 38.57575359506569 21.16361020327737 16.4465824945361 9 26.199743798395964 15.256470501109531 21.073243154365436 37.47054254612907 10 14.407284730260972 29.539225029821125 35.62031028077263 20.433179959145278 11 33.303651798568765 22.42420849485525 26.083016020094107 18.189123686481885 12 23.173946430413405 24.91908843010836 32.944123188361075 18.962841951117156 13 28.7784733848735 22.71236514347227 26.2378764675855 22.271285004068726 14 19.857652061294427 23.469336476637253 28.490173795671726 28.182837666396594 15 21.886678839034687 33.09660105284224 23.550666820657995 21.466053287465076 16 20.71662392231784 28.815539253241973 29.660228712388577 20.807608112051614 17 20.64938717430135 29.737034209268824 28.89903848697029 20.71454012945954 18 20.339462717200508 27.883392123343526 31.824144841187625 19.953000318268337 19 22.862028364786333 27.33177666290356 28.362670414195705 21.443524558114397 20 22.94544747634021 28.054217061323484 29.30455263386424 19.69578282847207 21 21.89804382094984 27.381745855418373 28.03045531328135 22.689755010350435 22 22.28761742703103 29.44119941986308 28.259545147965774 20.01163800514012 23 20.99435799303422 28.970575075633043 29.075930155312786 20.95913677601995 24 22.358147555812604 27.41650370908501 29.99492171864558 20.230427016456805 25 21.791645342068595 28.326075255663746 29.2275492206989 20.65473018156876 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 181.0 2 346.0 3 511.0 4 511.0 5 511.0 6 1516.5 7 2522.0 8 2522.0 9 2522.0 10 3704.0 11 4886.0 12 4886.0 13 4886.0 14 8207.5 15 11529.0 16 11529.0 17 11529.0 18 20873.5 19 30218.0 20 30218.0 21 30218.0 22 48569.5 23 66921.0 24 66921.0 25 66921.0 26 97932.5 27 128944.0 28 128944.0 29 128944.0 30 166934.0 31 204924.0 32 204924.0 33 204924.0 34 228771.0 35 252618.0 36 252618.0 37 252618.0 38 265374.0 39 278130.0 40 278130.0 41 278130.0 42 286191.5 43 294253.0 44 294253.0 45 294253.0 46 294219.5 47 294186.0 48 294186.0 49 294186.0 50 274930.5 51 255675.0 52 255675.0 53 255675.0 54 219512.5 55 183350.0 56 183350.0 57 183350.0 58 152922.5 59 122495.0 60 122495.0 61 122495.0 62 97905.0 63 73315.0 64 73315.0 65 73315.0 66 55499.5 67 37684.0 68 37684.0 69 37684.0 70 27225.0 71 16766.0 72 16766.0 73 16766.0 74 11530.5 75 6295.0 76 6295.0 77 6295.0 78 4246.0 79 2197.0 80 2197.0 81 2197.0 82 1426.0 83 655.0 84 655.0 85 655.0 86 427.5 87 200.0 88 200.0 89 200.0 90 126.5 91 53.0 92 53.0 93 53.0 94 33.5 95 14.0 96 14.0 97 14.0 98 9.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.455657109506349 3 0.0 4 0.0 5 4.8489636001526983E-4 6 0.001102037181852886 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.7632594909646176E-4 14 0.0 15 0.0 16 0.0 17 7.934667709340779E-4 18 0.0 19 3.9673338546703896E-4 20 6.612223091117316E-4 21 9.697927200305397E-4 22 0.004231822778315082 23 3.085704109188081E-4 24 0.0018514224655128485 25 8.375482582081932E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2268526.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.93561599836667 #Duplication Level Percentage of deduplicated Percentage of total 1 86.76473667475905 65.01768989659146 2 9.67396753867196 14.498494333171728 3 1.9927672473118958 4.479877236560593 4 0.6193870110972608 1.8565658887184164 5 0.2908069755562426 1.08958999249645 6 0.15054672473652952 0.6768786932801032 7 0.09237313657875189 0.4845426523861021 8 0.06932843781574752 0.41561353551419994 9 0.04548299088015288 0.3067466345147115 >10 0.26026199437810943 3.5201750577130064 >50 0.020054535445200427 1.0470760530870027 >100 0.016591110709310256 2.5265337278267954 >500 0.0017285974151075992 0.8826592182377623 >1k 0.0017285974151058682 2.221991066769013 >5k 2.3842722966977494E-4 0.9755660131327547 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5803 0.2558048706516919 No Hit CTGTAGGACGTGGAATATGGCAAGA 5471 0.2411698168766856 No Hit GTATCAACGCAGAGTACTTTTTTTT 5401 0.2380841127674975 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5166 0.22772496325808037 No Hit CTTTAGGACGTGAAATATGGCGAGG 4502 0.19845485570806773 No Hit GGTATCAACGCAGAGTACTTTTTTT 3323 0.1464827822118856 No Hit TATCAACGCAGAGTACTTTTTTTTT 3285 0.1448076856954692 No Hit GTCCTACAGTGTGCATTTCTCATTT 3201 0.14110484076444352 No Hit CTGAAGGACCTGGAATATGGCGAGA 2489 0.10971882182527332 No Hit CTGTAGGACCTGGAATATGGCGAGA 2372 0.1045612878142018 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.408148727411544E-5 6 0.0 0.0 0.0 4.408148727411544E-5 4.408148727411544E-5 7 0.0 0.0 0.0 4.408148727411544E-5 4.408148727411544E-5 8 0.0 0.0 0.0 4.408148727411544E-5 4.408148727411544E-5 9 0.0 0.0 0.0 8.816297454823088E-5 4.408148727411544E-5 10 0.0 0.0 0.0 8.816297454823088E-5 4.408148727411544E-5 11 0.0 0.0 0.0 1.7632594909646176E-4 4.408148727411544E-5 12 0.0 0.0 0.0 1.7632594909646176E-4 1.3224446182234632E-4 13 0.0 0.0 0.0 2.6448892364469264E-4 1.3224446182234632E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGTAC 25 0.004509794 19.980742 1 GGACCGT 40 0.005485979 14.1680565 6 GGTATCA 1110 0.0 12.600469 1 TAGACCG 60 4.297322E-4 12.593827 5 TAGGACC 1050 0.0 12.593826 4 CGGTAGG 90 4.2581505E-6 12.210454 1 AAGGCGT 155 1.8189894E-12 12.187575 6 GGCGAGG 1350 0.0 11.825246 19 GTCCTAT 290 0.0 11.023859 1 ATACCGT 60 0.006134771 11.019599 6 CGGCCAT 60 0.006135777 11.019356 12 GTATCAA 2455 0.0 10.905987 1 GATATAC 535 0.0 10.830683 1 TAGAACG 105 3.7158989E-6 10.794709 4 GTCCTAA 1665 0.0 10.7404 1 GGCGTGC 185 5.456968E-12 10.721536 8 GTTCTAG 290 0.0 10.679363 1 CCAACGA 160 6.020855E-10 10.626979 19 TGTAGGA 3205 0.0 10.567039 2 AGGACCT 2360 0.0 10.525985 5 >>END_MODULE