FastQCFastQC Report
Thu 26 May 2016
SRR1511813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268526
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68640.30257532864952835No Hit
CTGTAGGACGTGGAATATGGCAAGA64330.2835762076343846No Hit
GTCCTAAAGTGTGTATTTCTCATTT59560.26254933820463155No Hit
GTATCAACGCAGAGTACTTTTTTTT59400.2618440344082457No Hit
CTTTAGGACGTGAAATATGGCGAGG55090.24284491339310196No Hit
GGTATCAACGCAGAGTACTTTTTTT38940.17165331144540552No Hit
TATCAACGCAGAGTACTTTTTTTTT37450.16508516984156232No Hit
GTCCTACAGTGTGCATTTCTCATTT36180.15948682095774966No Hit
CTGAAGGACCTGGAATATGGCGAGA29770.13123058761504167No Hit
CTGTAGGACCTGGAATATGGCGAGA26360.11619880045456829No Hit
GTCCTTCAGTGTGCATTTCTCATTT23620.10412047294146067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG402.7680825E-416.62006417
CGTCGGT456.7758735E-414.77436719
GCGTCGG400.005288185614.24671118
TAGGACC11100.012.9232894
GGTATCA12850.012.099811
CCAACGA1103.8291546E-812.08811819
TGCGATA658.04594E-411.68883510
GGCGAGG14050.011.28920719
TCCAACG1701.4006218E-1010.61519618
TGTAGGA35300.010.52255252
AGGACCT21200.010.4409285
TGCACGC1106.0694856E-610.36055912
TAGAAAT9300.010.3170974
ACTGTTC5750.010.2404378
CTGTAGG34850.010.1820271
TGCACCG750.002648602210.1332285
CGAATAT750.002654906110.13032412
TGGCGAG33500.010.12154318
GTATCAA26150.010.1042051
GTAGGAC35650.010.0994743
Produced by FastQC (version 0.11.5)